ADF12_ARATH
ID ADF12_ARATH Reviewed; 137 AA.
AC Q8LFH6; A6QRD0; Q9FHC4;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Actin-depolymerizing factor 12;
DE Short=ADF-12;
DE Short=AtADF12;
GN Name=ADF12 {ECO:0000303|PubMed:16360805};
GN Synonyms=ADF10 {ECO:0000303|PubMed:21632657}; OrderedLocusNames=At5g52360;
GN ORFNames=K24M7.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista-Mercan V.R., Kim C.J., Chen H., Quan R., De Los Reyes C.,
RA Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=16360805; DOI=10.1016/j.jplph.2005.01.015;
RA Feng Y., Liu Q., Xue Q.;
RT "Comparative study of rice and Arabidopsis actin-depolymerizing factors
RT gene families.";
RL J. Plant Physiol. 163:69-79(2006).
RN [6]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=21632657; DOI=10.1093/pcp/pcr068;
RA Bou Daher F., van Oostende C., Geitmann A.;
RT "Spatial and temporal expression of actin depolymerizing factors ADF7 and
RT ADF10 during male gametophyte development in Arabidopsis thaliana.";
RL Plant Cell Physiol. 52:1177-1192(2011).
CC -!- FUNCTION: Actin-depolymerizing protein. Severs actin filaments (F-
CC actin) and binds to actin monomers. {ECO:0000250|UniProtKB:Q39251}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:21632657}. Note=In germinating pollen grains and
CC elongating pollen tubes, associates with the subapical actin fringe.
CC {ECO:0000269|PubMed:21632657}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in pollen.
CC {ECO:0000269|PubMed:21632657}.
CC -!- DEVELOPMENTAL STAGE: During male gametophyte development, expressed at
CC the polarized microspore stage, bicellular stage, mature pollen stage,
CC anthesis stage and open flower stage. Expressed in germinating pollen
CC grains and elongating pollen tubes. {ECO:0000269|PubMed:21632657}.
CC -!- SIMILARITY: Belongs to the actin-binding proteins ADF family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10533.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB019226; BAB10533.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96205.1; -; Genomic_DNA.
DR EMBL; BT030653; ABR46233.1; -; mRNA.
DR EMBL; AY084837; AAM61402.1; -; mRNA.
DR RefSeq; NP_568769.1; NM_124615.4.
DR AlphaFoldDB; Q8LFH6; -.
DR SMR; Q8LFH6; -.
DR STRING; 3702.AT5G52360.1; -.
DR PaxDb; Q8LFH6; -.
DR PRIDE; Q8LFH6; -.
DR ProteomicsDB; 244680; -.
DR EnsemblPlants; AT5G52360.1; AT5G52360.1; AT5G52360.
DR GeneID; 835312; -.
DR Gramene; AT5G52360.1; AT5G52360.1; AT5G52360.
DR KEGG; ath:AT5G52360; -.
DR Araport; AT5G52360; -.
DR TAIR; locus:2156727; AT5G52360.
DR eggNOG; KOG1735; Eukaryota.
DR HOGENOM; CLU_094004_2_2_1; -.
DR InParanoid; Q8LFH6; -.
DR OMA; VEDECKI; -.
DR OrthoDB; 1370477at2759; -.
DR PhylomeDB; Q8LFH6; -.
DR PRO; PR:Q8LFH6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8LFH6; baseline and differential.
DR Genevisible; Q8LFH6; AT.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0005884; C:actin filament; IDA:TAIR.
DR GO; GO:0048046; C:apoplast; IDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003779; F:actin binding; IDA:TAIR.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0030042; P:actin filament depolymerization; IDA:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR CDD; cd11286; ADF_cofilin_like; 1.
DR Gene3D; 3.40.20.10; -; 1.
DR InterPro; IPR002108; ADF-H.
DR InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR InterPro; IPR017904; ADF/Cofilin.
DR PANTHER; PTHR11913; PTHR11913; 1.
DR Pfam; PF00241; Cofilin_ADF; 1.
DR SMART; SM00102; ADF; 1.
DR PROSITE; PS51263; ADF_H; 1.
PE 2: Evidence at transcript level;
KW Actin-binding; Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
FT CHAIN 1..137
FT /note="Actin-depolymerizing factor 12"
FT /id="PRO_0000278103"
FT DOMAIN 7..137
FT /note="ADF-H"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00599"
FT MOD_RES 6
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q39250"
FT CONFLICT 136
FT /note="A -> T (in Ref. 4; AAM61402)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 137 AA; 15884 MW; 0E5DB2C913CADE3F CRC64;
MANAASGMAV EDECKLKFLE LKAKRNYRFI IFRIDGQQVV VEKLGSPQEN YDDFTNYLPP
NECRYAVYDF DFTTAENIQK SKIFFIAWSP DSSRVRMKMV YASSKDRFKR ELDGIQVELQ
ATDPSEMSLD IIKSRAL
