ID   DRT1_PHYBI              Reviewed;          77 AA.
AC   Q9PT75; P81566;
DT   04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Dermatoxin-B1 {ECO:0000303|PubMed:18644413};
DE            Short=DRT-B1 {ECO:0000303|PubMed:18644413};
DE   AltName: Full=Dermatoxin {ECO:0000303|PubMed:10880984};
DE   Flags: Precursor;
OS   Phyllomedusa bicolor (Two-colored leaf frog) (Rana bicolor).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Phyllomedusa.
OX   NCBI_TaxID=8393;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 45-76, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion;
RX   PubMed=10880984; DOI=10.1046/j.1432-1327.2000.01514.x;
RA   Amiche M., Seon A.A., Wroblewski H., Nicolas P.;
RT   "Isolation of dermatoxin from frog skin, an antibacterial peptide encoded
RT   by a novel member of the dermaseptin genes family.";
RL   Eur. J. Biochem. 267:4583-4592(2000).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18644413; DOI=10.1016/j.peptides.2008.06.017;
RA   Amiche M., Ladram A., Nicolas P.;
RT   "A consistent nomenclature of antimicrobial peptides isolated from frogs of
RT   the subfamily Phyllomedusinae.";
RL   Peptides 29:2074-2082(2008).
CC   -!- FUNCTION: Possesses a potent antimicrobial activity against Gram-
CC       positive bacteria B.megaterium, C.glutamicum and S.aureus and
CC       mollicutes A.laidlawii and S.melliferum (PubMed:10880984). Less active
CC       against Gram-negative bacteria B.cepacia, P.aeruginosa, S.typhimurium
CC       and S.meliloti. Probably acts by disturbing membrane functions with its
CC       amphipathic structure (PubMed:10880984). {ECO:0000269|PubMed:10880984}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10880984}. Target
CC       cell membrane {ECO:0000305|PubMed:10880984}.
CC   -!- TISSUE SPECIFICITY: Highest expression in skin and to a lesser extent
CC       in brain and intestine. {ECO:0000269|PubMed:10880984}.
CC   -!- MASS SPECTROMETRY: Mass=3194.72; Mass_error=0.1; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:10880984};
CC   -!- MISCELLANEOUS: The sequence shown corresponds to the dermatoxin
CC       isolated from frog skin. A variant of it is present in frog brain.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Dermatoxin subfamily. {ECO:0000305}.
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DR   EMBL; AJ251875; CAB63924.1; -; mRNA.
DR   AlphaFoldDB; Q9PT75; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   InterPro; IPR016322; FSAP.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
DR   PIRSF; PIRSF001822; Dermaseptin_precursor; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Direct protein sequencing; Membrane;
KW   Secreted; Signal; Target cell membrane; Target membrane.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..42
FT                   /evidence="ECO:0000305|PubMed:10880984"
FT                   /id="PRO_0000007112"
FT   PEPTIDE         45..76
FT                   /note="Dermatoxin-B1"
FT                   /evidence="ECO:0000269|PubMed:10880984"
FT                   /id="PRO_0000007113"
FT   REGION          24..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         76
FT                   /note="Glutamine amide"
FT                   /evidence="ECO:0000250|UniProtKB:Q5DVA5"
FT   VARIANT         21
FT                   /note="L -> I (in frog brain)"
FT   VARIANT         24
FT                   /note="S -> E (in frog brain)"
SQ   SEQUENCE   77 AA;  8378 MW;  7DECF8C5A3CDE3F6 CRC64;
     MAFLKKSLFL VLFLGLVPLS LCESEKREGE NEEEQEDDQS EEKRSLGSFL KGVGTTLASV
     GKVVSDQFGK LLQAGQG