ID   DRTI_DELRE              Reviewed;         185 AA.
AC   P83667;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Kunitz-type serine protease inhibitor DrTI;
OS   Delonix regia (Royal poinciana) (Poinciana regia).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Caesalpinioideae; Peltophorum clade;
OC   Delonix.
OX   NCBI_TaxID=72433 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Seed {ECO:0000269|PubMed:11397427};
RX   PubMed=11397427; DOI=10.1016/s0031-9422(01)00080-2;
RA   Pando S.C., Oliva M.L.V., Sampaio C.A.M., Di Ciero L., Novello J.C.,
RA   Marangoni S.;
RT   "Primary sequence determination of a Kunitz inhibitor isolated from Delonix
RT   regia seeds.";
RL   Phytochemistry 57:625-631(2001).
CC   -!- FUNCTION: Inhibits bovine trypsin and human plasma kallikrein.
CC       {ECO:0000269|PubMed:11397427}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
CC   -!- CAUTION: The reactive bond has a Glu in position P1 instead of an
CC       expected Arg or Lys. {ECO:0000305}.
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DR   PDB; 1R8N; X-ray; 1.75 A; A=1-185.
DR   PDBsum; 1R8N; -.
DR   AlphaFoldDB; P83667; -.
DR   SMR; P83667; -.
DR   EvolutionaryTrace; P83667; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond;
KW   Protease inhibitor; Secreted; Serine protease inhibitor.
FT   CHAIN           1..185
FT                   /note="Kunitz-type serine protease inhibitor DrTI"
FT                   /id="PRO_0000083324"
FT   SITE            68..69
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000255"
FT   DISULFID        44..89
FT                   /evidence="ECO:0000250"
FT   DISULFID        139..147
FT                   /evidence="ECO:0000250"
FT   STRAND          18..24
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          28..30
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          33..37
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          47..50
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          61..64
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          69..72
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          79..85
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          94..98
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          100..103
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          105..108
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   TURN            111..113
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   HELIX           116..118
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          122..128
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          130..132
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          134..143
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          148..153
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          155..157
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          159..162
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          164..167
FT                   /evidence="ECO:0007829|PDB:1R8N"
FT   STRAND          172..175
FT                   /evidence="ECO:0007829|PDB:1R8N"
SQ   SEQUENCE   185 AA;  20094 MW;  55CFF042CC50C7CC CRC64;
     SDAEKVYDIE GYPVFLGSEY YIVSAIIGAG GGGVRPGRTR GSMCPMSIIQ EQSDLQMGLP
     VRFSSPEESQ GKIYTDTELE IEFVEKPDCA ESSKWVIVKD SGEARVAIGG SEDHPQGELV
     RGFFKIEKLG SLAYKLVFCP KSSSGSCSDI GINYEGRRSL VLKSSDDSPF RVVFVKPRSG
     SETES