ID   DRUB_ECOU4              Reviewed;         548 AA.
AC   P0DW35;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Druantia protein DruB {ECO:0000303|PubMed:29371424};
GN   Name=druB {ECO:0000303|PubMed:29371424};
GN   ORFNames=H003_04355 {ECO:0000312|EMBL:ERA40830.1};
OS   Escherichia coli (strain UMEA 4076-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=1281278;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UMEA 4076-1;
RG   The Broad Institute Genome Sequencing Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Feldgarden M., Frimodt-Moller N., Leihof R.F., Rasmussen L., Young S.K.,
RA   Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA   Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA   Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA   Nusbaum C., Birren B.;
RT   "The Genome Sequence of Escherichia coli UMEA 4076-1.";
RL   Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=UMEA 4076-1;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Component of antiviral defense system Druantia type I,
CC       composed of DruA, DruB, DruC, DruD and DruE. Expression of Druantia in
CC       E.coli (strain MG1655) confers resistance to phage lambda, SECphi18,
CC       SECphi27 and T4. {ECO:0000269|PubMed:29371424}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the Druantia system
CC       does not confer resistance to SECphi27 in E.coli.
CC       {ECO:0000269|PubMed:29371424}.
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DR   EMBL; AWEM01000032; ERA40830.1; -; Genomic_DNA.
PE   4: Predicted;
KW   Antiviral defense; Cytoplasm.
FT   CHAIN           1..548
FT                   /note="Druantia protein DruB"
FT                   /id="PRO_0000456315"
SQ   SEQUENCE   548 AA;  60969 MW;  13616579AE244232 CRC64;
     MNYAIDKFTG TLILAARATK YAQYVCPVCK KGVNLRKGKV IPPYFAHLPG HGTSDCENFV
     PGNSIIIETI KTISKRYMDL RLLIPVGSNS REWSLELVLP TCNLCRAKIT LDVGGRSQTL
     DMRSMVKSRQ IGAELSVKSY RIVSYSGEPD PKFVTEVERE CPGLPSEGAA VFTALGRGAS
     KGFPRAQELR CTETFAFLWR HPVAPDFPDE LEIKSLASKQ GWNLALVTIP EVPSVESISW
     LKSFTYLPVV PARTSITAIW PFLNQKTSIN HVECVHSDTI LLSANMAPTS SENVGPTMYA
     QGSSLLLSAV GVEKSPAFFI LNPGENDFVG VSGSIEQDVN LFFSFYKKNV SVPRKYPSID
     LVFTKRNKEK TIVSLHQRRC IEVMMEARMF GHKLEYMSMP SGVEGVARIQ RQTESSVIKL
     VSNDDIAAHD KSMRLLSPVA LSQLSDCLAN LTCHVEIDFL GLGKIFLPSS SMLSLDDGEF
     IELSPNLRSR ILSFILQMGH TLHGFSLNND FLLVEKLVDL QPEPHLLPHY RALVKEVKTN
     GFECNRFR