ID   DRUE_ECOU4              Reviewed;        1836 AA.
AC   P0DW38;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Druantia protein DruE {ECO:0000303|PubMed:29371424};
DE   AltName: Full=Probable ATP-dependent helicase DruE {ECO:0000305};
GN   Name=druE {ECO:0000303|PubMed:29371424};
GN   ORFNames=H003_04358 {ECO:0000312|EMBL:ERA40833.1};
OS   Escherichia coli (strain UMEA 4076-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=1281278;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UMEA 4076-1;
RG   The Broad Institute Genome Sequencing Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Feldgarden M., Frimodt-Moller N., Leihof R.F., Rasmussen L., Young S.K.,
RA   Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA   Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA   Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA   Nusbaum C., Birren B.;
RT   "The Genome Sequence of Escherichia coli UMEA 4076-1.";
RL   Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=UMEA 4076-1;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Component of antiviral defense system Druantia type I,
CC       composed of DruA, DruB, DruC, DruD and DruE. Expression of Druantia in
CC       E.coli (strain MG1655) confers resistance to phage lambda, SECphi18,
CC       SECphi27 and T4 (PubMed:29371424). This protein is probably a helicase
CC       (Probable). {ECO:0000269|PubMed:29371424, ECO:0000305|PubMed:29371424}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the Druantia system
CC       does not confer resistance to SECphi27 in E.coli.
CC       {ECO:0000269|PubMed:29371424}.
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DR   EMBL; AWEM01000032; ERA40833.1; -; Genomic_DNA.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   4: Predicted;
KW   Antiviral defense; ATP-binding; Cytoplasm; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN           1..1836
FT                   /note="Druantia protein DruE"
FT                   /id="PRO_0000456319"
FT   DOMAIN          108..405
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          1014..1199
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           347..350
FT                   /note="DEAH box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   BINDING         121..128
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   1836 AA;  206036 MW;  4B45C12DD432060D CRC64;
     MINKNKVTER SGIHDTVKSL SENLRKYIEA QYHIRDEGLI AERRALLQQN ETIAQAPYIE
     ATPIYEPGAP YSELPIPEAA SNVLTQLSEL GIGLYQRPYK HQSQALESFL GEDASDLVIA
     TGTGSGKTES FLMPIIGKLA IESSERPKSA SLPGCRAILL YPMNALVNDQ LARIRRLFGD
     SEASKILRSG RCAPVRFGAY TGRTPYPGRR SSRRDELFIK PLFDEFYNKL ANNAPVRAEL
     NRIGRWPSKD LDAFYGQSAS QAKTYVSGKK TGKQFVLNNW GERLITQPED RELMTRHEMQ
     NRCPELLITN YSMLEYMLMR PIERNIFEQT KEWLKADEMN ELILVLDEAH MYRGAGGAEV
     ALLIRRLCAR LDIPRERMRC ILTSASLGSI EDGERFAQDL TGLSPTSSRK FRIIEGTRES
     RPESQIVTSK EANALAEFDL NSFQCVAEDL ESAYAAIESL AERMGWQKPM IKDHSTLRNW
     LFDNLTGFGP IETLIEIVSG KAVKLNILSE NLFPDSPQQI AERATDALLA LGCYAQRASD
     GRVLIPTRMH LFYRGLPGLY ACIDPDCNQR LGNHSGPTIL GRLYTKPLDQ CKCASKGRVY
     ELFTHRDCGA AFIRGYVSSE MDFVWHQPNG PLSEDEDIDL VPIDILVEET PHVHSDYQDR
     WLHIATGRLS KQCQDEDSGY RKVFIPDRVK SGSEITFDEC PVCMRKTRSA QNEPSKIMDH
     VTKGEAPFTT LVRTQISHQP ASRPIDGKHP NGGKKVLIFS DGRQKAARLA RDIPRDIELD
     LFRQSIALAC SKLKDINREP KPTSVLYLAF LSVLSEHDLL IFDGEDSRKV VMARDEFYRD
     YNSDLAQAFD DNFSPQESPS RYKIALLKLL CSNYYSLSGT TVGFVEPSQL KSKKMWEDVQ
     SKKLNIESKD VHALAVAWID TLLTEFAFDE SIDSTLRIKA AGFYKPTWGS QGRFGKALRK
     TLIQHPAMGE LYVEVLEEIF RTHLTLGKDG VYFLAPNALR LKIDLLHVWK QCNDCTALMP
     FALEHSTCLA CGSNSVKTVE PSESSYINAR KGFWRSPVEE VLVSNSRLLN LSVEEHTAQL
     SHRDRASVHA TTELYELRFQ DVLINDNDKP IDVLSCTTTM EVGVDIGSLV AVALRNVPPQ
     RENYQQRAGR AGRRGASVST VVTYSQNGPH DSYYFLNPER IVAGSPRTPE VKVNNPKIAR
     RHVHSFLVQT FFHELMEQGI YNPTEKTAIL EKALGTTRDF FHGAKDTGLN LDSFNNWVKN
     RILSTNGDLR TSVAAWLPPV LETGGLSASD WFAKVAEEFL NTLHGLAEIV PQIAALVDEE
     NEDDEQTSGG MKFAQEELLE FLFYHGLLPS YAFPTSLCSF LVEKIVKNIR GSFEVRTVQQ
     PQQSISQALS EYAPGRLIVI DRKTYRSGGV FSNALKGELN RARKLFNNPK KFIHCDKCSF
     VRDPHNNQNS ENTCPICGGI LKVEIMIQPE VFGPENAKEL NEDDREQEIT YVTAAQYPQP
     VDPEDFKFNN GGAHIVFTHA IDQKLVTVNR GKNEGGSSGF SVCCECGAAS VYDSYSPAKG
     AHERPYKYIA TKETPRLCSG EYKRVFLGHD FRTDLLLLRI TVGSPLVTDT SNAIVLRMYE
     DALYTIAEAL RLAASRHKQL DLDPAEFGSG FRILPTIEED TQALDLFLYD TLSGGAGYAE
     VAAANLDDIL TATLALLEGC ECDTSCTDCL NHFHNQHIQS RLDRKLGASL LRYALYGMVP
     RCASPDIQVE KLSQLRASLE LDGFQCLIKG TQEAPMIVSL NDRSVAVGSY PGLIDRPDFQ
     HDVYKSKNTN AHIAFNEYLL RSNLPQAHQN IRKLLR