ID   ADF1_YEAST              Reviewed;         113 AA.
AC   Q2V2Q1; D6VQV8;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 4.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Antisense of depressing factor protein 1 {ECO:0000303|PubMed:20195295};
GN   Name=ADF1 {ECO:0000303|PubMed:20195295};
GN   OrderedLocusNames=YCL058W-A {ECO:0000312|SGD:S000028518};
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=1574125; DOI=10.1038/357038a0;
RA   Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA   Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA   Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA   Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA   Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA   Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA   Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA   Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA   Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA   Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA   Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA   Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA   Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA   Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA   Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA   Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA   Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA   Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA   Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA   Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA   Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA   Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA   Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA   Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA   Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA   Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA   Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA   Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA   Sgouros J.G.;
RT   "The complete DNA sequence of yeast chromosome III.";
RL   Nature 357:38-46(1992).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   GENOME REANNOTATION.
RX   PubMed=12844361; DOI=10.1186/gb-2003-4-7-r45;
RA   Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A.,
RA   Gates K., Gaffney T.D., Philippsen P.;
RT   "Reinvestigation of the Saccharomyces cerevisiae genome annotation by
RT   comparison to the genome of a related fungus: Ashbya gossypii.";
RL   Genome Biol. 4:R45.1-R45.13(2003).
RN   [4]
RP   IDENTIFICATION OF PROBABLE INITIATION SITE.
RX   PubMed=12748633; DOI=10.1038/nature01644;
RA   Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT   "Sequencing and comparison of yeast species to identify genes and
RT   regulatory elements.";
RL   Nature 423:241-254(2003).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=20195295; DOI=10.1038/cr.2010.31;
RA   Li D., Dong Y., Jiang Y., Jiang H., Cai J., Wang W.;
RT   "A de novo originated gene depresses budding yeast mating pathway and is
RT   repressed by the protein encoded by its antisense strand.";
RL   Cell Res. 20:408-420(2010).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=29897761; DOI=10.1021/acs.jproteome.8b00032;
RA   He C., Jia C., Zhang Y., Xu P.;
RT   "Enrichment-based proteogenomics identifies microproteins, missing
RT   proteins, and novel smORFs in Saccharomyces cerevisiae.";
RL   J. Proteome Res. 17:2335-2344(2018).
CC   -!- FUNCTION: Transcriptional repressor which negatively regulates
CC       transcription of FYV5 by binding to the promoter on the sense strand.
CC       {ECO:0000269|PubMed:20195295}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20195295}.
CC   -!- MISCELLANEOUS: Encoded by the antisense strand of the FYV5 gene.
CC   -!- SIMILARITY: Belongs to the ADF1 family. {ECO:0000305}.
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DR   EMBL; X59720; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BK006937; DAA07427.1; -; Genomic_DNA.
DR   RefSeq; NP_001018031.2; NM_001184528.1.
DR   AlphaFoldDB; Q2V2Q1; -.
DR   SMR; Q2V2Q1; -.
DR   BioGRID; 37095; 210.
DR   STRING; 4932.YCL058W-A; -.
DR   iPTMnet; Q2V2Q1; -.
DR   MaxQB; Q2V2Q1; -.
DR   PaxDb; Q2V2Q1; -.
DR   PRIDE; Q2V2Q1; -.
DR   EnsemblFungi; YCL058W-A_mRNA; YCL058W-A; YCL058W-A.
DR   GeneID; 3289591; -.
DR   KEGG; sce:YCL058W-A; -.
DR   SGD; S000028518; ADF1.
DR   VEuPathDB; FungiDB:YCL058W-A; -.
DR   eggNOG; ENOG502SFBX; Eukaryota.
DR   HOGENOM; CLU_165604_1_0_1; -.
DR   InParanoid; Q2V2Q1; -.
DR   BioCyc; YEAST:G3O-29424-MON; -.
DR   PRO; PR:Q2V2Q1; -.
DR   Proteomes; UP000002311; Chromosome III.
DR   RNAct; Q2V2Q1; protein.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:SGD.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR   GO; GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IMP:SGD.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..113
FT                   /note="Antisense of depressing factor protein 1"
FT                   /id="PRO_0000248450"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   113 AA;  12739 MW;  0D5F95141CB29BFF CRC64;
     MGKCSMKKKG VGKNVGVGKK VQKKRSISTA ERKRTKLQVE KLNKSSETMI PTLLREASTQ
     EPAKLKAETT LKAEELIKDQ EKDSKVREQI RTEKSKTNDS MLKQIEMISG FSL