ID   ADF4_ORYSJ              Reviewed;         139 AA.
AC   Q84TB3; B7E3A0;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Actin-depolymerizing factor 4;
DE            Short=ADF-4;
DE            Short=OsADF4;
GN   Name=ADF4; OrderedLocusNames=Os03g0820600, LOC_Os03g60590;
GN   ORFNames=OJ1754_E06.22;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   GENE FAMILY.
RX   PubMed=16360805; DOI=10.1016/j.jplph.2005.01.015;
RA   Feng Y., Liu Q., Xue Q.;
RT   "Comparative study of rice and Arabidopsis actin-depolymerizing factors
RT   gene families.";
RL   J. Plant Physiol. 163:69-79(2006).
RN   [7]
RP   FUNCTION, INTERACTION WITH LECRK1, AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Dongjin;
RX   PubMed=24033867; DOI=10.1111/tpj.12328;
RA   Cheng X., Wu Y., Guo J., Du B., Chen R., Zhu L., He G.;
RT   "A rice lectin receptor-like kinase that is involved in innate immune
RT   responses also contributes to seed germination.";
RL   Plant J. 76:687-698(2013).
CC   -!- FUNCTION: Actin-depolymerizing protein. Severs actin filaments (F-
CC       actin) and binds to actin monomers (By similarity). Involved in innate
CC       immunity. Required for the expression of defense-related genes PR1A,
CC       LOX2 and CHS1 upon biotic stresses. Required for basal resistance to
CC       the fungal blast (Magnaporthe grisea), bacterial blight (Xanthomonas
CC       oryzae pv. oryzae, Xoo) and the herbivorous insect brown planthopper
CC       (Nilaparvata lugens, BPH). Involved in the promotion of seed
CC       germination. Required for the expression of alpha-amylase genes during
CC       seed germination (PubMed:24033867). {ECO:0000250|UniProtKB:Q9ZSK3,
CC       ECO:0000269|PubMed:24033867}.
CC   -!- SUBUNIT: Interacts with LECRK1 (via kinase domain).
CC       {ECO:0000269|PubMed:24033867}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q9ZSK3}.
CC   -!- DISRUPTION PHENOTYPE: Decreased germination rates due to decreased
CC       alpha-amylase activity during seed germination. Increased
CC       susceptibility to infection by the fungal blast (Magnaporthe grisea),
CC       bacterial blight (Xanthomonas oryzae pv. oryzae, Xoo) and the
CC       herbivorous insect brown planthopper (Nilaparvata lugens, BPH). Almost
CC       no induction of the defense-related genes PR1A, LOX2 and CHS1 after
CC       exposure to biotic stresses. {ECO:0000269|PubMed:24033867}.
CC   -!- SIMILARITY: Belongs to the actin-binding proteins ADF family.
CC       {ECO:0000305}.
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DR   EMBL; AC104433; AAO65864.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF99588.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF13635.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS87084.1; -; Genomic_DNA.
DR   EMBL; AK058941; BAG86847.1; -; mRNA.
DR   RefSeq; XP_015632174.1; XM_015776688.1.
DR   AlphaFoldDB; Q84TB3; -.
DR   SMR; Q84TB3; -.
DR   STRING; 4530.OS03T0820600-01; -.
DR   PaxDb; Q84TB3; -.
DR   PRIDE; Q84TB3; -.
DR   EnsemblPlants; Os03t0820600-01; Os03t0820600-01; Os03g0820600.
DR   GeneID; 4334595; -.
DR   Gramene; Os03t0820600-01; Os03t0820600-01; Os03g0820600.
DR   KEGG; osa:4334595; -.
DR   eggNOG; KOG1735; Eukaryota.
DR   HOGENOM; CLU_094004_2_2_1; -.
DR   InParanoid; Q84TB3; -.
DR   OMA; HRFITYK; -.
DR   OrthoDB; 1370477at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   ExpressionAtlas; Q84TB3; baseline and differential.
DR   Genevisible; Q84TB3; OS.
DR   GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR   GO; GO:0030042; P:actin filament depolymerization; IBA:GO_Central.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   CDD; cd11286; ADF_cofilin_like; 1.
DR   Gene3D; 3.40.20.10; -; 1.
DR   InterPro; IPR002108; ADF-H.
DR   InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR   InterPro; IPR017904; ADF/Cofilin.
DR   PANTHER; PTHR11913; PTHR11913; 1.
DR   Pfam; PF00241; Cofilin_ADF; 1.
DR   SMART; SM00102; ADF; 1.
DR   PROSITE; PS51263; ADF_H; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; Cytoplasm; Cytoskeleton; Plant defense; Reference proteome.
FT   CHAIN           1..139
FT                   /note="Actin-depolymerizing factor 4"
FT                   /id="PRO_0000278107"
FT   DOMAIN          5..139
FT                   /note="ADF-H"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00599"
SQ   SEQUENCE   139 AA;  15946 MW;  B876B938462C8F1B CRC64;
     MANSSSGVAI HDDCKLKFNE LQSKRMHRFI TFMMDNKGKE IIVDKIGDRT TSYEDFTSSL
     PEGDCRFAIY DFDFLTAEDV PKSRIFYILW SPDNAKVRSK MLYASSNERF KKELNGIQLE
     VQATDAGEIS LDALKDRVK