ADF7_ARATH
ID ADF7_ARATH Reviewed; 137 AA.
AC Q67ZM4; O65603; Q7FEI4;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Actin-depolymerizing factor 7;
DE Short=ADF-7;
DE Short=AtADF7;
GN Name=ADF7; OrderedLocusNames=At4g25590; ORFNames=M7J2.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-137.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=16360805; DOI=10.1016/j.jplph.2005.01.015;
RA Feng Y., Liu Q., Xue Q.;
RT "Comparative study of rice and Arabidopsis actin-depolymerizing factors
RT gene families.";
RL J. Plant Physiol. 163:69-79(2006).
RN [6]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=21632657; DOI=10.1093/pcp/pcr068;
RA Bou Daher F., van Oostende C., Geitmann A.;
RT "Spatial and temporal expression of actin depolymerizing factors ADF7 and
RT ADF10 during male gametophyte development in Arabidopsis thaliana.";
RL Plant Cell Physiol. 52:1177-1192(2011).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=24058157; DOI=10.1105/tpc.113.117820;
RA Zheng Y., Xie Y., Jiang Y., Qu X., Huang S.;
RT "Arabidopsis actin-depolymerizing factor7 severs actin filaments and
RT regulates actin cable turnover to promote normal pollen tube growth.";
RL Plant Cell 25:3405-3423(2013).
CC -!- FUNCTION: Actin-depolymerizing protein. Severs actin filaments (F-
CC actin) and binds to actin monomers. Binds monomeric actin (G-actin)
CC with a marked preference for the ADP-loaded form and inhibits the rate
CC of nucleotide exchange on G-actin. Required for pollen tube growth.
CC Promotes turnover of longitudinal actin cables by severing actin
CC filaments in pollen tubes. {ECO:0000269|PubMed:24058157}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:21632657, ECO:0000269|PubMed:24058157}. Note=In
CC germinating pollen grains and elongating pollen tubes, associates with
CC the subapical actin fringe (PubMed:21632657). Associates with actin
CC cables in the shanks of pollen tubes (PubMed:24058157).
CC {ECO:0000269|PubMed:21632657, ECO:0000269|PubMed:24058157}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in pollen.
CC {ECO:0000269|PubMed:21632657}.
CC -!- DEVELOPMENTAL STAGE: During male gametophyte development, expressed at
CC the early microspore stage just after tetrad separation, polarized
CC microspore stage, bicellular stage, mature pollen stage, anthesis stage
CC and open flower stage. Expressed in germinating pollen grains and
CC elongating pollen tubes. {ECO:0000269|PubMed:21632657}.
CC -!- DISRUPTION PHENOTYPE: Decreased actin filament severing frequency.
CC Increased amount of filamentous actin and inhibition of pollen tube
CC growth. {ECO:0000269|PubMed:24058157}.
CC -!- SIMILARITY: Belongs to the actin-binding proteins ADF family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA18167.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB81369.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL022197; CAA18167.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161563; CAB81369.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85080.1; -; Genomic_DNA.
DR EMBL; AK176093; BAD43856.1; -; mRNA.
DR EMBL; BT010513; AAQ65136.1; -; mRNA.
DR PIR; T05788; T05788.
DR RefSeq; NP_001329121.1; NM_001341753.1.
DR RefSeq; NP_194289.2; NM_118691.3.
DR AlphaFoldDB; Q67ZM4; -.
DR SMR; Q67ZM4; -.
DR STRING; 3702.AT4G25590.1; -.
DR PaxDb; Q67ZM4; -.
DR PRIDE; Q67ZM4; -.
DR ProteomicsDB; 244817; -.
DR EnsemblPlants; AT4G25590.1; AT4G25590.1; AT4G25590.
DR GeneID; 828664; -.
DR Gramene; AT4G25590.1; AT4G25590.1; AT4G25590.
DR KEGG; ath:AT4G25590; -.
DR Araport; AT4G25590; -.
DR TAIR; locus:2131879; AT4G25590.
DR eggNOG; KOG1735; Eukaryota.
DR HOGENOM; CLU_094004_2_2_1; -.
DR InParanoid; Q67ZM4; -.
DR OMA; CRYLLYD; -.
DR OrthoDB; 1370477at2759; -.
DR PhylomeDB; Q67ZM4; -.
DR PRO; PR:Q67ZM4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q67ZM4; baseline and differential.
DR Genevisible; Q67ZM4; AT.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0005884; C:actin filament; IDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0030042; P:actin filament depolymerization; IDA:TAIR.
DR CDD; cd11286; ADF_cofilin_like; 1.
DR Gene3D; 3.40.20.10; -; 1.
DR InterPro; IPR002108; ADF-H.
DR InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR InterPro; IPR017904; ADF/Cofilin.
DR PANTHER; PTHR11913; PTHR11913; 1.
DR Pfam; PF00241; Cofilin_ADF; 1.
DR SMART; SM00102; ADF; 1.
DR PROSITE; PS51263; ADF_H; 1.
PE 2: Evidence at transcript level;
KW Actin-binding; Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
FT CHAIN 1..137
FT /note="Actin-depolymerizing factor 7"
FT /id="PRO_0000278099"
FT DOMAIN 7..137
FT /note="ADF-H"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00599"
FT MOD_RES 6
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q39250"
SQ SEQUENCE 137 AA; 15820 MW; A6484EF92403FDFD CRC64;
MANAASGMAV EDECKLKFLE LKSKRNYRFI IFRIDGQQVV VEKLGNPDET YDDFTASLPA
NECRYAVFDF DFITDENCQK SKIFFIAWSP DSSRVRMKMV YASSKDRFKR ELDGIQVELQ
ATDPSEMSFD IIKSRAL
