DSCR6_XENLA
ID DSCR6_XENLA Reviewed; 170 AA.
AC B7XDF1;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Protein ripply3 {ECO:0000303|PubMed:19247927};
DE Short=XRipply3 {ECO:0000303|PubMed:19247927};
DE AltName: Full=Down syndrome critical region protein 6 homolog;
GN Name=ripply3; Synonyms=dscr6;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAH14956.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TBX1 AND TLE4,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=19247927; DOI=10.1387/ijdb.082823kh;
RA Hitachi K., Danno H., Tazumi S., Aihara Y., Uchiyama H., Okabayashi K.,
RA Kondow A., Asashima M.;
RT "The Xenopus Bowline/Ripply family proteins negatively regulate the
RT transcriptional activity of T-box transcription factors.";
RL Int. J. Dev. Biol. 53:631-639(2009).
RN [2]
RP FUNCTION, INDUCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 40-TRP--TRP-44
RP AND 105-PHE--GLN-109.
RX PubMed=22354841; DOI=10.1242/dev.071456;
RA Janesick A., Shiotsugu J., Taketani M., Blumberg B.;
RT "RIPPLY3 is a retinoic acid-inducible repressor required for setting the
RT borders of the pre-placodal ectoderm.";
RL Development 139:1213-1224(2012).
CC -!- FUNCTION: Acts as a transcriptional corepressor. Negative regulator of
CC the transcriptional activity of tbx1 that plays a key role in
CC pharyngeal development. Plays a role in the formation of the
CC anteroposterior (AP) axis during embryonic development; required to
CC establish the posterolateral border of the pre-placodal ectoderm (PPE)
CC acting downstream of the retinoic acid receptor (RAR) signaling.
CC {ECO:0000269|PubMed:19247927, ECO:0000269|PubMed:22354841}.
CC -!- SUBUNIT: Interacts with tbx1 and tle4/grg4.
CC {ECO:0000269|PubMed:19247927}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19247927}.
CC -!- TISSUE SPECIFICITY: At neurula stage, expressed in the region close to
CC the heart mesoderm. At the tailbud stage, expressed in the pharyngeal
CC region. {ECO:0000269|PubMed:19247927}.
CC -!- DEVELOPMENTAL STAGE: Maternally expressed. Ubiquitously expressed until
CC stage 16. Expressed in the pre-placodal ectoderm domain at stage 18.
CC Expressed in the epibranchial placodes of the tailbud embryos.
CC Expressed in the pronephros. {ECO:0000269|PubMed:22354841}.
CC -!- INDUCTION: Up-regulated by retinoc acid (RA) in the pre-placodal
CC ectoderm (PPE) during development. {ECO:0000269|PubMed:22354841}.
CC -!- DOMAIN: The Ripply homology domain and the WRPW motif are both
CC necessary for its transcriptional corepressor activity on the
CC transcription activator tbx1.
CC -!- DOMAIN: The WRPW motif is required for binding to tle/groucho proteins.
CC {ECO:0000250|UniProtKB:Q25QX6}.
CC -!- SIMILARITY: Belongs to the ripply family. {ECO:0000255}.
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DR EMBL; AB455086; BAH14956.1; -; mRNA.
DR RefSeq; NP_001153161.1; NM_001159689.1.
DR AlphaFoldDB; B7XDF1; -.
DR GeneID; 100294521; -.
DR KEGG; xla:100294521; -.
DR CTD; 100294521; -.
DR Xenbase; XB-GENE-6372204; ripply3.S.
DR OrthoDB; 1549120at2759; -.
DR Proteomes; UP000186698; Chromosome 2S.
DR Bgee; 100294521; Expressed in neurula embryo and 9 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:UniProtKB.
DR GO; GO:0009948; P:anterior/posterior axis specification; IMP:GO_Central.
DR GO; GO:0071300; P:cellular response to retinoic acid; IDA:UniProtKB.
DR GO; GO:0060788; P:ectodermal placode formation; IMP:UniProtKB.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR InterPro; IPR028127; Ripply_fam.
DR PANTHER; PTHR16770; PTHR16770; 1.
DR Pfam; PF14998; Ripply; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..170
FT /note="Protein ripply3"
FT /id="PRO_0000401112"
FT REGION 51..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..114
FT /note="Ripply homology domain"
FT /evidence="ECO:0000255"
FT REGION 142..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 40..43
FT /note="WRPW motif"
FT /evidence="ECO:0000255"
FT COMPBIAS 142..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 40..43
FT /note="WRPW->AAAA: Inhibits transcriptional repression
FT activity on TBX1; when associated with 105-A--A-109."
FT MUTAGEN 105..108
FT /note="FPVQ->AAAA: Inhibits transcriptional repression
FT activity on TBX1; when associated with 40-A--A-44."
SQ SEQUENCE 170 AA; 18941 MW; B3BBFE0D61CD6C22 CRC64;
MDSSQYMLKA TLTQMCLCSR GVRNVHSEHP QQQDSSLTLW RPWLLGAGDR ELDGQQRRSG
EADGVPTNTG PKGALGFQHP VRLYMPKSKT SEYLQHMGRK VLASFPVQAT IHFYNDDSDS
EEEDEEEEME FYNYYQNCAA NGVDSSRGSS DNYSVQGGPK RNIGSHAGSA
