DSF_DROME
ID DSF_DROME Reviewed; 691 AA.
AC Q9VML1; O96680;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Protein dissatisfaction {ECO:0000303|PubMed:9023356};
DE AltName: Full=Nuclear receptor subfamily 2 group E member 4 {ECO:0000312|EMBL:AAF52303.1};
GN Name=dsf {ECO:0000303|PubMed:9023356, ECO:0000312|FlyBase:FBgn0015381};
GN Synonyms=NR2E4 {ECO:0000312|EMBL:AAF52303.1};
GN ORFNames=CG9019 {ECO:0000312|FlyBase:FBgn0015381};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:AAD05225.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND MUTAGENESIS OF 544-GLN--LYS-691.
RX PubMed=9883729; DOI=10.1016/s0896-6273(00)80655-8;
RA Finley K.D., Edeen P.T., Foss M., Gross E., Ghbeish N., Palmer R.H.,
RA Taylor B.J., McKeown M.;
RT "Dissatisfaction encodes a tailless-like nuclear receptor expressed in a
RT subset of CNS neurons controlling Drosophila sexual behavior.";
RL Neuron 21:1363-1374(1998).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF 544-GLN--LYS-691.
RX PubMed=9023356; DOI=10.1073/pnas.94.3.913;
RA Finley K.D., Taylor B.J., Milstein M., McKeown M.;
RT "dissatisfaction, a gene involved in sex-specific behavior and neural
RT development of Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:913-918(1997).
RN [5] {ECO:0000305}
RP FUNCTION, REGION, AND MUTAGENESIS OF HIS-24; 102-LEU--ILE-322;
RP 103-HIS--LYS-691; 172-THR--ILE-322; 173-GLN--LYS-691; 244-SER--LYS-691;
RP 429-SER--LYS-691 AND 527-HIS--LYS-691.
RX PubMed=11977984; DOI=10.1006/dbio.2002.0648;
RA Pitman J.L., Tsai C.C., Edeen P.T., Finley K.D., Evans R.M., McKeown M.;
RT "DSF nuclear receptor acts as a repressor in culture and in vivo.";
RL Dev. Biol. 245:315-328(2002).
CC -!- FUNCTION: Orphan receptor that binds DNA as a monomer to hormone
CC response elements (HRE) containing an extended core motif half-site
CC sequence 5'-AAGTCA-3' (PubMed:11977984). Acts as a transcriptional
CC repressor (PubMed:11977984). In both females and males, functions
CC downstream of tra2 to regulate the development and function of certain
CC sex-specific abdominal neurons, and likely regulates the development
CC and/or function of the nervous system that controls courtship behaviors
CC involved in the recognition and response of adults to appropriate
CC sexual partners (PubMed:9883729, PubMed:9023356, PubMed:11977984). In
CC males, promotes abdominal motor neuronal innervation of the ventral
CC muscles of abdominal segment 5 which is required for correct abdominal
CC curling during copulation (PubMed:9883729, PubMed:9023356). In females,
CC also promotes abdominal motor neuronal innervation but on the uterine
CC muscles which is required for egg laying (PubMed:9883729,
CC PubMed:9023356). {ECO:0000269|PubMed:11977984,
CC ECO:0000269|PubMed:9023356, ECO:0000269|PubMed:9883729}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:11977984}.
CC -!- TISSUE SPECIFICITY: In larvae, pupae and adults, expressed in small
CC subsets of cells in the anterior region of the brain (PubMed:9883729).
CC In pharate adult brains, expressed in around 20 large neurons located
CC close to the antennal lobe and lateral protocerebral neurophils. Also
CC expressed in a small cluster of cells located just below the
CC chemosensory region of the antennal lobe and near the mechanosensory
CC region of the antennal lobe and subesophageal neuropils
CC (PubMed:9883729). {ECO:0000269|PubMed:9883729}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development and in adults.
CC {ECO:0000269|PubMed:9883729}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR2
CC subfamily. {ECO:0000305}.
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DR EMBL; AF106677; AAD05225.1; -; mRNA.
DR EMBL; AE014134; AAF52303.1; -; Genomic_DNA.
DR EMBL; AE014134; AGB92645.1; -; Genomic_DNA.
DR RefSeq; NP_001260109.1; NM_001273180.1.
DR RefSeq; NP_477140.1; NM_057792.3.
DR AlphaFoldDB; Q9VML1; -.
DR SMR; Q9VML1; -.
DR STRING; 7227.FBpp0305445; -.
DR PaxDb; Q9VML1; -.
DR EnsemblMetazoa; FBtr0079145; FBpp0078776; FBgn0015381.
DR EnsemblMetazoa; FBtr0333245; FBpp0305445; FBgn0015381.
DR GeneID; 33823; -.
DR KEGG; dme:Dmel_CG9019; -.
DR UCSC; CG9019-RA; d. melanogaster.
DR CTD; 33823; -.
DR FlyBase; FBgn0015381; dsf.
DR VEuPathDB; VectorBase:FBgn0015381; -.
DR eggNOG; KOG3575; Eukaryota.
DR HOGENOM; CLU_007368_20_3_1; -.
DR InParanoid; Q9VML1; -.
DR OMA; SPDKCQE; -.
DR OrthoDB; 870262at2759; -.
DR PhylomeDB; Q9VML1; -.
DR BioGRID-ORCS; 33823; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33823; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0015381; Expressed in male reproductive gland and 1 other tissue.
DR ExpressionAtlas; Q9VML1; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:FlyBase.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0003707; F:nuclear steroid receptor activity; NAS:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0007620; P:copulation; NAS:FlyBase.
DR GO; GO:0007619; P:courtship behavior; TAS:FlyBase.
DR GO; GO:0008049; P:male courtship behavior; TAS:FlyBase.
DR GO; GO:0007617; P:mating behavior; NAS:FlyBase.
DR GO; GO:0048047; P:mating behavior, sex discrimination; IMP:FlyBase.
DR GO; GO:0018991; P:oviposition; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045924; P:regulation of female receptivity; IMP:FlyBase.
DR GO; GO:0035206; P:regulation of hemocyte proliferation; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:FlyBase.
DR GO; GO:0007530; P:sex determination; TAS:FlyBase.
DR GO; GO:0018993; P:somatic sex determination; NAS:FlyBase.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Receptor; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..691
FT /note="Protein dissatisfaction"
FT /id="PRO_0000452342"
FT DOMAIN 452..685
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 10..89
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 13..33
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 49..72
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 85..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 103..162
FT /note="Impairs binding to DNA"
FT /evidence="ECO:0000269|PubMed:11977984"
FT REGION 172..488
FT /note="Impairs binding to DNA"
FT /evidence="ECO:0000269|PubMed:11977984"
FT REGION 172..265
FT /note="Repressive Function 2 (RF2), important for
FT transcriptional repression"
FT /evidence="ECO:0000269|PubMed:11977984"
FT REGION 176..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 295..450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 674..685
FT /note="AF-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT COMPBIAS 96..112
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..136
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 238..253
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 24
FT /note="H->Y: In dsf7, greatly decreased DNA binding of
FT fragment 1-102."
FT /evidence="ECO:0000269|PubMed:11977984"
FT MUTAGEN 102..322
FT /note="Missing: Strong increase in DNA binding."
FT /evidence="ECO:0000269|PubMed:11977984"
FT MUTAGEN 103..691
FT /note="Missing: Very strong increase in DNA binding."
FT /evidence="ECO:0000269|PubMed:11977984"
FT MUTAGEN 172..322
FT /note="Missing: Slight increase in DNA binding."
FT /evidence="ECO:0000269|PubMed:11977984"
FT MUTAGEN 173..691
FT /note="Missing: Increased DNA binding."
FT /evidence="ECO:0000269|PubMed:11977984"
FT MUTAGEN 244..691
FT /note="Missing: Slight increase in DNA binding."
FT /evidence="ECO:0000269|PubMed:11977984"
FT MUTAGEN 429..691
FT /note="Missing: Reduced DNA binding and weakly represses
FT DNA transcription."
FT /evidence="ECO:0000269|PubMed:11977984"
FT MUTAGEN 527..691
FT /note="Missing: Reduced DNA binding."
FT /evidence="ECO:0000269|PubMed:11977984"
FT MUTAGEN 544..691
FT /note="Missing: In dsf1; males and females are sterile due
FT to abnormal courtship behaviors and defects in their sex-
FT specific nervous system. Females display active resistance
FT and rejection behaviors during courtship and copulation.
FT Synapses are also absent on the circumferential muscles of
FT the uterus which likely results in the observed egg-laying
FT defect in which the mature eggs reach the uterus but
FT females are unable to lay them. Males actively court both
FT females and males, and during the final step of courtship
FT display defects in abdominal curling which is required to
FT make genital-genital contact with females. The observed
FT slow abdominal bends during mating is likely due, at least
FT in part, to abnormal innervation of the ventral
FT longitudinal muscles of abdominal segment 5 (A5)."
FT /evidence="ECO:0000269|PubMed:9023356,
FT ECO:0000269|PubMed:9883729"
FT CONFLICT 237
FT /note="V -> I (in Ref. 1; AAD05225)"
FT /evidence="ECO:0000305"
FT CONFLICT 300
FT /note="T -> A (in Ref. 1; AAD05225)"
FT /evidence="ECO:0000305"
FT CONFLICT 417
FT /note="N -> NNN (in Ref. 1; AAD05225)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 691 AA; 74053 MW; 6D7983DE08D6583F CRC64;
MGTAGDRLLD IPCKVCGDRS SGKHYGIYSC DGCSGFFKRS IHRNRIYTCK ATGDLKGRCP
VDKTHRNQCR ACRLAKCFQS AMNKDAVQHE RGPRKPKLHP QLHHHHHHAA AAAAAAHHAA
AAHHHHHHHH HAHAAAAHHA AVAAAAASGL HHHHHAMPVS LVTNVSASFN YTQHISTHPP
APAAPPSGFH LTASGAQQGP APPAGHLHHG GAGHQHATAF HHPGHGHALP APHGGVVSNP
GGNSSAISGS GPGSTLPFPS HLLHHNLIAE AASKLPGITA TAVAAVVSST STPYASAAQT
SSPSSNNHNY SSPSPSNSIQ SISSIGSRSG GGEEGLSLGS ESPRVNVETE TPSPSNSPPL
SAGSISPAPT LTTSSGSPQH RQMSRHSLSE ATTPPSHASL MICASNNNNN NNNNNNNGEH
KQSSYTSGSP TPTTPTPPPP RSGVGSTCNT ASSSSGFLEL LLSPDKCQEL IQYQVQHNTL
LFPQQLLDSR LLSWEMLQET TARLLFMAVR WVKCLMPFQT LSKNDQHLLL QESWKELFLL
NLAQWTIPLD LTPILESPLI RERVLQDEAT QTEMKTIQEI LCRFRQITPD GSEVGCMKAI
ALFAPETAGL CDVQPVEMLQ DQAQCILSDH VRLRYPRQAT RFGRLLLLLP SLRTIRAATI
EALFFKETIG NVPIARLLRD MYTMEPAQVD K
