DSO2_DROME
ID DSO2_DROME Reviewed; 46 AA.
AC P83869; D1Z361; Q2PE21;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Daisho2 {ECO:0000303|PubMed:32038657};
DE AltName: Full=Immune-induced peptide 14 {ECO:0000303|PubMed:14645501};
DE Short=DIM-14 {ECO:0000305|PubMed:9736738};
DE Short=DIM4 {ECO:0000303|PubMed:9736738};
DE Flags: Precursor;
GN Name=Dso2 {ECO:0000303|PubMed:32038657, ECO:0000312|FlyBase:FBgn0067905};
GN Synonyms=IM14 {ECO:0000303|PubMed:14645501};
GN ORFNames=CG33990 {ECO:0000312|FlyBase:FBgn0067905};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000305};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ACZ61124.1, ECO:0000312|EMBL:ANY27909.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Frise E., Park S., Wan K., Yu C., Wan K., Booth B., Spirohn K.,
RA Hao T., Hu Y., Calderwood M., Hill D., Mohr S., Vidal M., Celniker S.,
RA Perrimon N.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PROTEIN SEQUENCE OF 23-46, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, MASS SPECTROMETRY, AND INDUCTION.
RC STRAIN=Oregon-R {ECO:0000303|PubMed:14645501};
RC TISSUE=Hemolymph {ECO:0000303|PubMed:14645501};
RX PubMed=14645501; DOI=10.1074/mcp.m300114-mcp200;
RA Levy F., Bulet P., Ehret-Sabatier L.;
RT "Proteomic analysis of the systemic immune response of Drosophila.";
RL Mol. Cell. Proteomics 3:156-166(2004).
RN [5] {ECO:0000305}
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY BACTERIA, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Oregon-R {ECO:0000303|PubMed:9736738};
RC TISSUE=Hemolymph {ECO:0000303|PubMed:9736738};
RX PubMed=9736738; DOI=10.1073/pnas.95.19.11342;
RA Uttenweiler-Joseph S., Moniatte M., Lagueux M., van Dorsselaer A.,
RA Hoffmann J.A., Bulet P.;
RT "Differential display of peptides induced during the immune response of
RT Drosophila: a matrix-assisted laser desorption ionization time-of-flight
RT mass spectrometry study.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:11342-11347(1998).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY BACTERIA,
RP MASS SPECTROMETRY, AND DISRUPTION PHENOTYPE.
RX PubMed=32038657; DOI=10.3389/fimmu.2020.00009;
RA Cohen L.B., Lindsay S.A., Xu Y., Lin S.J.H., Wasserman S.A.;
RT "The Daisho Peptides Mediate Drosophila Defense Against a Subset of
RT Filamentous Fungi.";
RL Front. Immunol. 11:9-9(2020).
CC -!- FUNCTION: Peptide which plays a role in the humoral immune response to
CC a subset of filamentous fungi, including F.oxysporum and
CC F.verticillioides. {ECO:0000269|PubMed:32038657}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:14645501,
CC ECO:0000269|PubMed:32038657, ECO:0000269|PubMed:9736738}.
CC -!- TISSUE SPECIFICITY: Hemolymph (at protein level).
CC {ECO:0000269|PubMed:14645501, ECO:0000269|PubMed:32038657,
CC ECO:0000269|PubMed:9736738}.
CC -!- DEVELOPMENTAL STAGE: Adult. {ECO:0000269|PubMed:14645501}.
CC -!- INDUCTION: By bacterial and fungal infection (at protein level)
CC (PubMed:14645501, PubMed:9736738, PubMed:32038657). Detected within 24
CC hours of bacterial infection (at protein level) (PubMed:14645501,
CC PubMed:9736738). Induced by Gram-positive bacteria M.luteus (at protein
CC level) (PubMed:32038657). {ECO:0000269|PubMed:14645501,
CC ECO:0000269|PubMed:32038657, ECO:0000269|PubMed:9736738}.
CC -!- MASS SPECTROMETRY: Mass=2694.01; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:14645501};
CC -!- MASS SPECTROMETRY: Mass=2694; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:32038657};
CC -!- DISRUPTION PHENOTYPE: Adult males infected with spores from F.oxysporum
CC or F.verticillioides display reduced survival.
CC {ECO:0000269|PubMed:32038657}.
CC -!- MISCELLANEOUS: 'Daisho' is the Japanese term for a matched pair of
CC samurai swords, one short and one long, and refers to the role of the
CC two Daisho peptides (Dso1 and Dso2) in defense against fungal
CC infection. {ECO:0000303|PubMed:32038657}.
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DR EMBL; AE013599; ABC66114.1; -; Genomic_DNA.
DR EMBL; BT100325; ACZ61124.1; -; mRNA.
DR EMBL; KX532099; ANY27909.1; -; mRNA.
DR RefSeq; NP_001033962.1; NM_001038873.2.
DR AlphaFoldDB; P83869; -.
DR BioGRID; 566827; 1.
DR STRING; 7227.FBpp0099553; -.
DR PaxDb; P83869; -.
DR DNASU; 3885603; -.
DR EnsemblMetazoa; FBtr0100038; FBpp0099553; FBgn0067905.
DR GeneID; 3885603; -.
DR KEGG; dme:Dmel_CG33990; -.
DR CTD; 3885603; -.
DR FlyBase; FBgn0067905; Dso2.
DR VEuPathDB; VectorBase:FBgn0067905; -.
DR HOGENOM; CLU_3191859_0_0_1; -.
DR PhylomeDB; P83869; -.
DR BioGRID-ORCS; 3885603; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 3885603; -.
DR PRO; PR:P83869; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0067905; Expressed in seminal fluid secreting gland and 11 other tissues.
DR ExpressionAtlas; P83869; baseline.
DR GO; GO:0005576; C:extracellular region; IDA:FlyBase.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0006952; P:defense response; IDA:FlyBase.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:1905036; P:positive regulation of antifungal innate immune response; IMP:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; HEP:FlyBase.
DR GO; GO:0008063; P:Toll signaling pathway; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Immunity; Innate immunity; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:14645501"
FT PEPTIDE 23..46
FT /note="Daisho2"
FT /id="PRO_0000021505"
SQ SEQUENCE 46 AA; 4995 MW; 72DAA5E6AA58F445 CRC64;
MNCLKICGFF FALIAALATA EAGTQVIHAG GHTLIQTDRS QYIRKN
