DSX_DROME
ID DSX_DROME Reviewed; 549 AA.
AC P23023; P23022; Q0KIA7; Q95TA5; Q9VHY0;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 213.
DE RecName: Full=Protein doublesex;
GN Name=dsx; ORFNames=CG11094;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS FEMALE AND MALE).
RC TISSUE=Larva, and Pupae;
RX PubMed=2493994; DOI=10.1016/0092-8674(89)90633-8;
RA Burtis K.C., Baker B.S.;
RT "Drosophila doublesex gene controls somatic sexual differentiation by
RT producing alternatively spliced mRNAs encoding related sex-specific
RT polypeptides.";
RL Cell 56:997-1010(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM FEMALE).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP DNA-BINDING.
RX PubMed=1907913; DOI=10.1002/j.1460-2075.1991.tb07798.x;
RA Burtis K.C., Coschigano K.T., Baker B.S., Wensink P.C.;
RT "The doublesex proteins of Drosophila melanogaster bind directly to a sex-
RT specific yolk protein gene enhancer.";
RL EMBO J. 10:2577-2582(1991).
RN [6]
RP DNA-BINDING DOMAIN, AND MUTAGENESIS.
RX PubMed=8440242; DOI=10.1002/j.1460-2075.1993.tb05684.x;
RA Erdman S.E., Burtis K.C.;
RT "The Drosophila doublesex proteins share a novel zinc finger related DNA
RT binding domain.";
RL EMBO J. 12:527-535(1993).
RN [7]
RP FUNCTION.
RC STRAIN=Canton-S;
RX PubMed=12435630; DOI=10.1101/gad.1010302;
RA Dauwalder B., Tsujimoto S., Moss J., Mattox W.;
RT "The Drosophila takeout gene is regulated by the somatic sex-determination
RT pathway and affects male courtship behavior.";
RL Genes Dev. 16:2879-2892(2002).
CC -!- FUNCTION: Controls somatic sexual differentiation. Binds directly and
CC specifically to the FBE (fat body enhancer) of the yolk protein 1 and 2
CC genes (Yp1 and Yp2). This enhancer is sufficient to direct the female-
CC specific transcription characteristic of the Yp genes in adult fat
CC bodies. Involved in regulation of male-specific expression of takeout
CC in brain-associated fat body. {ECO:0000269|PubMed:12435630}.
CC -!- INTERACTION:
CC P23023-2; Q01645: Mst84Dd; NbExp=4; IntAct=EBI-15110079, EBI-201877;
CC P23023-2; P08175: Mst87F; NbExp=4; IntAct=EBI-15110079, EBI-131659;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Male; Synonyms=A;
CC IsoId=P23023-1; Sequence=Displayed;
CC Name=Female; Synonyms=B, C;
CC IsoId=P23023-2; Sequence=VSP_001321, VSP_001322;
CC -!- MISCELLANEOUS: Experimentally shown to bind zinc.
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DR EMBL; M25292; AAA17840.1; -; mRNA.
DR EMBL; M25293; AAA17841.1; -; mRNA.
DR EMBL; M25294; AAA17842.1; -; mRNA.
DR EMBL; AE014297; AAF54169.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13385.1; -; Genomic_DNA.
DR EMBL; AY060257; AAL25296.1; -; mRNA.
DR EMBL; BT029258; ABK30895.1; -; mRNA.
DR PIR; A32372; A32372.
DR PIR; B32372; B32372.
DR RefSeq; NP_001262352.1; NM_001275423.1. [P23023-1]
DR RefSeq; NP_001287220.1; NM_001300291.1. [P23023-2]
DR RefSeq; NP_524272.4; NM_079548.5. [P23023-2]
DR RefSeq; NP_731197.1; NM_169202.1. [P23023-1]
DR RefSeq; NP_731198.1; NM_169203.2. [P23023-2]
DR PDB; 1LPV; NMR; -; A=35-86.
DR PDB; 1ZV1; X-ray; 1.60 A; A/B=350-397.
DR PDB; 2JZ0; NMR; -; A/B=350-397.
DR PDB; 2JZ1; NMR; -; A/B=350-397.
DR PDBsum; 1LPV; -.
DR PDBsum; 1ZV1; -.
DR PDBsum; 2JZ0; -.
DR PDBsum; 2JZ1; -.
DR AlphaFoldDB; P23023; -.
DR SMR; P23023; -.
DR BioGRID; 66125; 66.
DR DIP; DIP-17110N; -.
DR IntAct; P23023; 35.
DR STRING; 7227.FBpp0081256; -.
DR PaxDb; P23023; -.
DR DNASU; 40940; -.
DR EnsemblMetazoa; FBtr0081759; FBpp0081256; FBgn0000504. [P23023-1]
DR EnsemblMetazoa; FBtr0081760; FBpp0081257; FBgn0000504. [P23023-2]
DR EnsemblMetazoa; FBtr0081761; FBpp0081258; FBgn0000504. [P23023-2]
DR EnsemblMetazoa; FBtr0330073; FBpp0303106; FBgn0000504. [P23023-1]
DR EnsemblMetazoa; FBtr0339710; FBpp0308767; FBgn0000504. [P23023-2]
DR GeneID; 40940; -.
DR KEGG; dme:Dmel_CG11094; -.
DR UCSC; CG11094-RA; d. melanogaster. [P23023-1]
DR CTD; 40940; -.
DR FlyBase; FBgn0000504; dsx.
DR VEuPathDB; VectorBase:FBgn0000504; -.
DR eggNOG; KOG3815; Eukaryota.
DR InParanoid; P23023; -.
DR OMA; HTSGAPM; -.
DR PhylomeDB; P23023; -.
DR SignaLink; P23023; -.
DR BioGRID-ORCS; 40940; 0 hits in 3 CRISPR screens.
DR EvolutionaryTrace; P23023; -.
DR GenomeRNAi; 40940; -.
DR PRO; PR:P23023; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000504; Expressed in spermathecum and 24 other tissues.
DR ExpressionAtlas; P23023; baseline and differential.
DR Genevisible; P23023; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0008270; F:zinc ion binding; IDA:FlyBase.
DR GO; GO:0016199; P:axon midline choice point recognition; IMP:FlyBase.
DR GO; GO:0007619; P:courtship behavior; NAS:FlyBase.
DR GO; GO:0045497; P:female analia development; NAS:FlyBase.
DR GO; GO:0046660; P:female sex differentiation; TAS:FlyBase.
DR GO; GO:0019101; P:female somatic sex determination; TAS:FlyBase.
DR GO; GO:0035215; P:genital disc development; IMP:FlyBase.
DR GO; GO:0007486; P:imaginal disc-derived female genitalia development; IMP:FlyBase.
DR GO; GO:0007485; P:imaginal disc-derived male genitalia development; IGI:FlyBase.
DR GO; GO:0045496; P:male analia development; NAS:FlyBase.
DR GO; GO:0008049; P:male courtship behavior; TAS:FlyBase.
DR GO; GO:0045433; P:male courtship behavior, veined wing generated song production; TAS:FlyBase.
DR GO; GO:0046661; P:male sex differentiation; TAS:FlyBase.
DR GO; GO:0048086; P:negative regulation of developmental pigmentation; TAS:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; NAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; TAS:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; NAS:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0045498; P:sex comb development; TAS:FlyBase.
DR GO; GO:0007530; P:sex determination; TAS:FlyBase.
DR GO; GO:0007548; P:sex differentiation; IBA:GO_Central.
DR GO; GO:0048071; P:sex-specific pigmentation; TAS:FlyBase.
DR GO; GO:0018993; P:somatic sex determination; TAS:FlyBase.
DR Gene3D; 4.10.1040.10; -; 1.
DR InterPro; IPR001275; DM_DNA-bd.
DR InterPro; IPR036407; DM_DNA-bd_sf.
DR InterPro; IPR026607; DMRT.
DR InterPro; IPR014932; DSX_dimer.
DR PANTHER; PTHR12322; PTHR12322; 1.
DR Pfam; PF00751; DM; 1.
DR Pfam; PF08828; DSX_dimer; 1.
DR SMART; SM00301; DM; 1.
DR SMART; SM01143; DSX_dimer; 1.
DR SUPFAM; SSF82927; SSF82927; 1.
DR PROSITE; PS40000; DM_1; 1.
DR PROSITE; PS50809; DM_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Differentiation; DNA-binding;
KW Metal-binding; Nucleus; Reference proteome; Sexual differentiation;
KW Transcription; Transcription regulation; Zinc.
FT CHAIN 1..549
FT /note="Protein doublesex"
FT /id="PRO_0000207041"
FT DNA_BIND 44..91
FT /note="DM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00070"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 129..331
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 452..549
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..151
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 206..222
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..237
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 288..331
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 460..517
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 398..427
FT /note="ARVEINRTVAQIYYNYYTPMALVNGAPMYL -> GQYVVNEYSRQHNLNIYD
FT GGELRNTTRQCG (in isoform Female)"
FT /evidence="ECO:0000303|PubMed:12537569,
FT ECO:0000303|PubMed:2493994"
FT /id="VSP_001321"
FT VAR_SEQ 428..549
FT /note="Missing (in isoform Female)"
FT /evidence="ECO:0000303|PubMed:12537569,
FT ECO:0000303|PubMed:2493994"
FT /id="VSP_001322"
FT MUTAGEN 47
FT /note="C->A,H: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:8440242"
FT MUTAGEN 50
FT /note="H->Y: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:8440242"
FT MUTAGEN 59
FT /note="H->Y: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:8440242"
FT MUTAGEN 68
FT /note="C->D,Y: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:8440242"
FT MUTAGEN 70
FT /note="C->Y: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:8440242"
FT MUTAGEN 91
FT /note="R->Q: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:8440242"
FT CONFLICT 53
FT /note="K -> N (in Ref. 4; AAL25296)"
FT /evidence="ECO:0000305"
FT HELIX 45..48
FT /evidence="ECO:0007829|PDB:1LPV"
FT TURN 49..51
FT /evidence="ECO:0007829|PDB:1LPV"
FT TURN 55..58
FT /evidence="ECO:0007829|PDB:1LPV"
FT HELIX 60..62
FT /evidence="ECO:0007829|PDB:1LPV"
FT TURN 64..67
FT /evidence="ECO:0007829|PDB:1LPV"
FT HELIX 71..80
FT /evidence="ECO:0007829|PDB:1LPV"
FT HELIX 354..366
FT /evidence="ECO:0007829|PDB:1ZV1"
FT HELIX 371..373
FT /evidence="ECO:0007829|PDB:1ZV1"
FT HELIX 374..383
FT /evidence="ECO:0007829|PDB:1ZV1"
FT TURN 384..386
FT /evidence="ECO:0007829|PDB:1ZV1"
FT HELIX 388..407
FT /evidence="ECO:0007829|PDB:1ZV1"
FT STRAND 411..413
FT /evidence="ECO:0007829|PDB:2JZ1"
SQ SEQUENCE 549 AA; 57409 MW; 3C1B92724E4CE083 CRC64;
MVSEENWNSD TMSDSDMIDS KNDVCGGASS SSGSSISPRT PPNCARCRNH GLKITLKGHK
RYCKFRYCTC EKCRLTADRQ RVMALQTALR RAQAQDEQRA LHMHEVPPAN PAATTLLSHH
HHVAAPAHVH AHHVHAHHAH GGHHSHHGHV LHHQQAAAAA AAAPSAPASH LGGSSTAASS
IHGHAHAHHV HMAAAAAASV AQHQHQSHPH SHHHHHQNHH QHPHQQPATQ TALRSPPHSD
HGGSVGPATS SSGGGAPSSS NAAAATSSNG SSGGGGGGGG GSSGGGAGGG RSSGTSVITS
ADHHMTTVPT PAQSLEGSCD SSSPSPSSTS GAAILPISVS VNRKNGANVP LGQDVFLDYC
QKLLEKFRYP WELMPLMYVI LKDADANIEE ASRRIEEARV EINRTVAQIY YNYYTPMALV
NGAPMYLTYP SIEQGRYGAH FTHLPLTQIC PPTPEPLALS RSPSSPSGPS AVHNQKPSRP
GSSNGTVHSA ASPTMVTTMA TTSSTPTLSR RQRSRSATPT TPPPPPPAHS SSNGAYHHGH
HLVSSTAAT
