DSZB_RHOSH
ID DSZB_RHOSH Reviewed; 365 AA.
AC Q6WNP2;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 21.
DE RecName: Full=2'-hydroxybiphenyl-2-sulfinate desulfinase {ECO:0000305};
DE EC=3.13.1.3 {ECO:0000250|UniProtKB:P54997, ECO:0000305|PubMed:16820450};
DE AltName: Full=2-(2-hydroxyphenyl)benzenesulfinate desulfinase {ECO:0000305};
DE Short=HPBS desulfinase {ECO:0000305};
DE AltName: Full=Dibenzothiophene desulfurization enzyme B {ECO:0000303|PubMed:16820450};
GN Name=dszB {ECO:0000303|PubMed:16820450};
OS Rhodococcus erythropolis (strain XP).
OC Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus;
OC Rhodococcus erythropolis group.
OX NCBI_TaxID=1078016;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE FUNCTION, PATHWAY, AND
RP BIOTECHNOLOGY.
RC STRAIN=XP;
RX PubMed=16820450; DOI=10.1128/aem.00081-06;
RA Tao F., Yu B., Xu P., Ma C.Q.;
RT "Biodesulfurization in biphasic systems containing organic solvents.";
RL Appl. Environ. Microbiol. 72:4604-4609(2006).
CC -!- FUNCTION: Catalyzes the third and final step of the '4S'
CC desulfurization pathway that removes covalently bound sulfur from
CC dibenzothiophene (DBT) without breaking carbon-carbon bonds. Oxidizes
CC 2-(2'-hydroxyphenyl)benzene sulphinate (HBPS) to 2-hydroxybiphenyl
CC (HBP) plus sulfite. The rate-limiting step of the '4S' desulfurization
CC pathway. {ECO:0000250|UniProtKB:P54997, ECO:0000305|PubMed:16820450}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2'-hydroxybiphenyl-2-sulfinate + H2O = biphenyl-2-ol + H(+) +
CC sulfite; Xref=Rhea:RHEA:12945, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17043, ChEBI:CHEBI:17359, ChEBI:CHEBI:18218; EC=3.13.1.3;
CC Evidence={ECO:0000250|UniProtKB:P54997, ECO:0000305|PubMed:16820450};
CC -!- PATHWAY: Sulfur metabolism; dibenzothiophene degradation.
CC {ECO:0000269|PubMed:16820450}.
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P54997}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P54997}.
CC -!- BIOTECHNOLOGY: Can be used to remove sulfur from polycyclic aromatic
CC sulfur compounds found in gasoline and diesel (biodesulfurization),
CC which are a considerable source of pollution. As the substrates are not
CC very soluble in conventional media, biphasic systems may help improve
CC catalysis. Expression of dszD-dszA-dszB-dszC (cloned in this order) in
CC organic-solvent-tolerant P.putida strain Idaho allows P.putida to grow
CC on 10% p-xylene with DBT as the sole sulfur source. In this P.putida
CC strain 97% of DBT was degraded, 71% of 4,6-dimethyldibenzothiophene and
CC about 50% of 3-methyldibenzothiophene or 4-methyldibenzothiophene was
CC degraded in the presence of 10% p-xylene. Degradation of DBT in the
CC presence of 10% of other organic solvents was tested; when grown in
CC dodecane, cyclohexane or heptanol bacteria metabolized DBT as well as
CC p-xylene, while other organic solvents degraded DBT slightly less well.
CC {ECO:0000269|PubMed:16820450}.
CC -!- SIMILARITY: Belongs to the DszB desulfinase family. {ECO:0000305}.
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DR EMBL; AY278323; AAP33511.1; -; Genomic_DNA.
DR SMR; Q6WNP2; -.
DR UniPathway; UPA00346; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0018896; P:dibenzothiophene catabolic process; IEA:UniProtKB-UniPathway.
PE 1: Evidence at protein level;
KW Cytoplasm; Hydrolase; Monooxygenase; Oxidoreductase.
FT CHAIN 1..365
FT /note="2'-hydroxybiphenyl-2-sulfinate desulfinase"
FT /id="PRO_0000455395"
FT ACT_SITE 27
FT /evidence="ECO:0000250|UniProtKB:P54997"
FT ACT_SITE 70
FT /evidence="ECO:0000250|UniProtKB:P54997"
FT BINDING 27
FT /ligand="2'-hydroxybiphenyl-2-sulfinate"
FT /ligand_id="ChEBI:CHEBI:18218"
FT /evidence="ECO:0000250|UniProtKB:P54997"
FT BINDING 60
FT /ligand="2'-hydroxybiphenyl-2-sulfinate"
FT /ligand_id="ChEBI:CHEBI:18218"
FT /evidence="ECO:0000250|UniProtKB:P54997"
FT BINDING 70
FT /ligand="2'-hydroxybiphenyl-2-sulfinate"
FT /ligand_id="ChEBI:CHEBI:18218"
FT /evidence="ECO:0000250|UniProtKB:P54997"
FT SITE 60
FT /note="May orient the sulfinate group"
FT /evidence="ECO:0000250|UniProtKB:P54997"
SQ SEQUENCE 365 AA; 39045 MW; DA6A867756DA23D6 CRC64;
MTSRVDPANP GSELDSAIRD TLTYSNCPVP NALLTASESG FLDAAGIELD VLSGQQGTVH
FTYDQPAYTR FGGEIPPLLS EGLRAPGRTR LLGITPLLGR QGFFVRDDSP ITAAADLAGR
RIGVSASAIR ILRGQLGDYL ELDPWRQTLV ALGSWEARAL LHTLEHGELG VDDVELVPIS
SPGVDVPAEQ LEESATVKGA DLFPDVARGQ AAVLASGDVD ALYSWLPWAG ELQATGARPV
VDLGLDERNA YASVWTVSSG LVRQRPGLVQ RLVDAAVDAG LWARDHSDAV TSLHAANLGV
STGAVGQGFG ADFQQRLVPR LDHDALALLE RTQQFLLTNN LLQEPVALDQ WAAPEFLNNS
LNRHR