ID   ADH1_CLOSA              Reviewed;         388 AA.
AC   P13604;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=NADPH-dependent butanol dehydrogenase;
DE            Short=BDH;
DE            EC=1.1.1.-;
GN   Name=adh1;
OS   Clostridium saccharobutylicum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=169679;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-117 / DSM 13864 / NCP 262;
RX   PubMed=2673928; DOI=10.1016/0378-1119(89)90238-2;
RA   Youngleson J.S., Jones W.A., Jones D.T., Woods D.R.;
RT   "Molecular analysis and nucleotide sequence of the adh1 gene encoding an
RT   NADPH-dependent butanol dehydrogenase in the Gram-positive anaerobe
RT   Clostridium acetobutylicum.";
RL   Gene 78:355-364(1989).
CC   -!- FUNCTION: This enzyme has activity using butanol and ethanol as
CC       substrates.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to originate from C.acetobutylicum.
CC       {ECO:0000305|PubMed:2673928}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M26941; AAA83520.1; -; Genomic_DNA.
DR   RefSeq; WP_022743700.1; NZ_JABUEU010000008.1.
DR   AlphaFoldDB; P13604; -.
DR   SMR; P13604; -.
DR   STRING; 169679.CSACC_03550; -.
DR   GeneID; 55472924; -.
DR   BioCyc; MetaCyc:BDHNADPHCLOS-MON; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:UniProt.
DR   CDD; cd08179; NADPH_BDH; 1.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR018211; ADH_Fe_CS.
DR   InterPro; IPR039697; Alcohol_dehydrogenase_Fe.
DR   InterPro; IPR034802; NADPH_BDH.
DR   PANTHER; PTHR11496; PTHR11496; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00913; ADH_IRON_1; 1.
DR   PROSITE; PS00060; ADH_IRON_2; 1.
PE   3: Inferred from homology;
KW   NADP; Oxidoreductase.
FT   CHAIN           1..388
FT                   /note="NADPH-dependent butanol dehydrogenase"
FT                   /id="PRO_0000087821"
SQ   SEQUENCE   388 AA;  43274 MW;  BBFDD458BFAB2C1B CRC64;
     MMRFTLPRDI YYGKGSLEQL KNLKGKKAML VLGGGSMKRF GFVDKVLGYL KEAGIEVKLI
     EGVEPDPSVE TVFKGAELMR QFEPDWIIAM GGGSPIDAAK AMWIFYEHPE KTFDDIKDPF
     TVPELRNKAK FLAIPSTSGT ATEVTAFSVI TDYKTEIKYP LADFNITPDV AVVDSELAET
     MPPKLTAHTG MDALTHAIEA YVATLHSPFT DPLAMQAIEM INEHLFKSYE GDKEAREQMH
     YAQCLAGMAF SNALLGICHS MAHKTGAVFH IPHGCANAIY LPYVIKFNSK TSLERYAKIA
     KQISLAGNTN EELVDSLINL VKELNKKMQI PTTLKEYGIH EQEFKNKVDL ISERAIGDAC
     TGSNPRQLNK DEMKKIFECV YYGTEVDF