ADH2_DROMO
ID ADH2_DROMO Reviewed; 254 AA.
AC P09369; Q6YFE3; Q867G0; Q86LV9;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 3.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Alcohol dehydrogenase 2;
DE EC=1.1.1.1;
GN Name=Adh2; ORFNames=GI17643;
OS Drosophila mojavensis (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila.
OX NCBI_TaxID=7230;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS SER-28; THR-92; LEU-112;
RP THR-115; ILE-136; LEU-146; PRO-147; GLN-226 AND LEU-236.
RC STRAIN=VRF;
RX PubMed=3224808; DOI=10.1093/genetics/120.3.713;
RA Atkinson P.W., Mills L.E., Starmer W.T., Sullivan D.T.;
RT "Structure and evolution of the Adh genes of Drosophila mojavensis.";
RL Genetics 120:713-723(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS SER-28; THR-92; LEU-112;
RP THR-115; ILE-136; LEU-146; PRO-147; GLN-226 AND LEU-236.
RC STRAIN=VRF;
RX PubMed=1317314; DOI=10.1093/genetics/131.1.143;
RA Bayer C.A., Curtiss S.W., Weaver J.A., Sullivan D.T.;
RT "Delineation of cis-acting sequences required for expression of Drosophila
RT mojavensis Adh-1.";
RL Genetics 131:143-153(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS SER-28 AND SER-71.
RC STRAIN=MJBC 103.3, MJBC 108.5, MJBC 115.4, MJBC 157.3, MJBC 202.2,
RC MJBC 205.1, MJBC 25.3, MJBC 33.3, MJBC 35.1, MJBC 39.3, MJBC 47.3, MJBC 80,
RC and MJBC 95.1;
RX PubMed=12586706; DOI=10.1093/genetics/163.1.181;
RA Matzkin L.M., Eanes W.F.;
RT "Sequence variation of alcohol dehydrogenase (Adh) paralogs in cactophilic
RT Drosophila.";
RL Genetics 163:181-194(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS SER-28; TYR-98; THR-115;
RP ILE-136 AND LEU-236.
RC STRAIN=MJS 115, MJS 122, MJS 127, MJS 132, MJS 133, MJS 14, MJS 147.2F,
RC MJS 154, MJS 16F, MJS 36, MJS 58.2, MJS 68, MJS 84, MJS 87, and MJS 93;
RX PubMed=14660699; DOI=10.1093/molbev/msh019;
RA Matzkin L.M.;
RT "Population genetics and geographic variation of alcohol dehydrogenase
RT (Adh) paralogs and glucose-6-phosphate dehydrogenase (G6pd) in Drosophila
RT mojavensis.";
RL Mol. Biol. Evol. 21:276-285(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson 15081-1352.22;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; M37276; AAA28334.1; -; Genomic_DNA.
DR EMBL; X12536; CAA31054.1; -; Genomic_DNA.
DR EMBL; AY154864; AAN86900.1; -; Genomic_DNA.
DR EMBL; AY154865; AAN86901.1; -; Genomic_DNA.
DR EMBL; AY154866; AAN86902.1; -; Genomic_DNA.
DR EMBL; AY154867; AAN86903.1; -; Genomic_DNA.
DR EMBL; AY154868; AAN86904.1; -; Genomic_DNA.
DR EMBL; AY154869; AAN86905.1; -; Genomic_DNA.
DR EMBL; AY154870; AAN86906.1; -; Genomic_DNA.
DR EMBL; AY154871; AAN86907.1; -; Genomic_DNA.
DR EMBL; AY154872; AAN86908.1; -; Genomic_DNA.
DR EMBL; AY154873; AAN86909.1; -; Genomic_DNA.
DR EMBL; AY154874; AAN86910.1; -; Genomic_DNA.
DR EMBL; AY154875; AAN86911.1; -; Genomic_DNA.
DR EMBL; AY154876; AAN86912.1; -; Genomic_DNA.
DR EMBL; AY364508; AAR21255.1; -; Genomic_DNA.
DR EMBL; AY364509; AAR21256.1; -; Genomic_DNA.
DR EMBL; AY364510; AAR21257.1; -; Genomic_DNA.
DR EMBL; AY364511; AAR21258.1; -; Genomic_DNA.
DR EMBL; AY364512; AAR21259.1; -; Genomic_DNA.
DR EMBL; AY364513; AAR21260.1; -; Genomic_DNA.
DR EMBL; AY364514; AAR21261.1; -; Genomic_DNA.
DR EMBL; AY364515; AAR21262.1; -; Genomic_DNA.
DR EMBL; AY364516; AAR21263.1; -; Genomic_DNA.
DR EMBL; AY364517; AAR21264.1; -; Genomic_DNA.
DR EMBL; AY364518; AAR21265.1; -; Genomic_DNA.
DR EMBL; AY364519; AAR21266.1; -; Genomic_DNA.
DR EMBL; AY364520; AAR21267.1; -; Genomic_DNA.
DR EMBL; AY364521; AAR21268.1; -; Genomic_DNA.
DR EMBL; AY364522; AAR21269.1; -; Genomic_DNA.
DR EMBL; CH933807; EDW12371.1; -; Genomic_DNA.
DR PIR; S01901; S01901.
DR RefSeq; XP_002002929.1; XM_002002893.2.
DR AlphaFoldDB; P09369; -.
DR SMR; P09369; -.
DR STRING; 7230.FBpp0166860; -.
DR EnsemblMetazoa; FBtr0168368; FBpp0166860; FBgn0012567.
DR GeneID; 6576952; -.
DR KEGG; dmo:Dmoj_GI17643; -.
DR eggNOG; KOG4169; Eukaryota.
DR HOGENOM; CLU_010194_2_16_1; -.
DR InParanoid; P09369; -.
DR OMA; TTLQCAQ; -.
DR OrthoDB; 1053465at2759; -.
DR PhylomeDB; P09369; -.
DR Proteomes; UP000009192; Unassembled WGS sequence.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0006066; P:alcohol metabolic process; IEA:InterPro.
DR InterPro; IPR002425; ADH_Drosophila-type.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR01168; ALCDHDRGNASE.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW NAD; Oxidoreductase; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..254
FT /note="Alcohol dehydrogenase 2"
FT /id="PRO_0000054479"
FT ACT_SITE 151
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 10..33
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 138
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VARIANT 28
FT /note="R -> S (in strain: MJBC 95.1, MJS 93 and VRF)"
FT /evidence="ECO:0000269|PubMed:12586706,
FT ECO:0000269|PubMed:1317314, ECO:0000269|PubMed:14660699,
FT ECO:0000269|PubMed:3224808"
FT VARIANT 71
FT /note="T -> S (in strain: MJBC 33.3)"
FT /evidence="ECO:0000269|PubMed:12586706"
FT VARIANT 92
FT /note="A -> T (in strain: VRF)"
FT /evidence="ECO:0000269|PubMed:1317314,
FT ECO:0000269|PubMed:3224808"
FT VARIANT 98
FT /note="H -> Y (in strain: MJS 115, MJS 147.2F, MJS 16F, MJS
FT 122, MJS 127, MJS 132, MJS 14, MJS 154, MJS 36, MJS 58.2,
FT MJS 68, MJS 84 and MJS 87)"
FT /evidence="ECO:0000269|PubMed:14660699"
FT VARIANT 112
FT /note="V -> L (in strain: VRF)"
FT /evidence="ECO:0000269|PubMed:1317314,
FT ECO:0000269|PubMed:3224808"
FT VARIANT 115
FT /note="I -> T (in strain: MJS 115, MJS 122, MJS 127, MJS
FT 132, MJS 133, MJS 14, MJS 147.2F, MJS 154, MJS 16F, MJS 36,
FT MJS 58.2, MJS 68, MJS 84, MJS 87, MJS 93 and VRF)"
FT /evidence="ECO:0000269|PubMed:1317314,
FT ECO:0000269|PubMed:14660699, ECO:0000269|PubMed:3224808"
FT VARIANT 136
FT /note="V -> I (in strain: MJS 115, MJS 122, MJS 127, MJS
FT 132, MJS 133, MJS 14, MJS 147.2F, MJS 154, MJS 16F, MJS 36,
FT MJS 58.2, MJS 68, MJS 84, MJS 87, MJS 93 and VRF)"
FT /evidence="ECO:0000269|PubMed:1317314,
FT ECO:0000269|PubMed:14660699, ECO:0000269|PubMed:3224808"
FT VARIANT 146
FT /note="Y -> L (in strain: VRF)"
FT /evidence="ECO:0000269|PubMed:1317314,
FT ECO:0000269|PubMed:3224808"
FT VARIANT 147
FT /note="Q -> P (in strain: VRF)"
FT /evidence="ECO:0000269|PubMed:1317314,
FT ECO:0000269|PubMed:3224808"
FT VARIANT 226
FT /note="E -> Q (in strain: VRF)"
FT /evidence="ECO:0000269|PubMed:1317314,
FT ECO:0000269|PubMed:3224808"
FT VARIANT 236
FT /note="V -> L (in strain: MJS 115, MJS 122, MJS 127, MJS
FT 132, MJS 133, MJS 14, MJS 147.2F, MJS 154, MJS 16F, MJS 36,
FT MJS 58.2, MJS 68, MJS 84, MJS 87, MJS 93 and VRF)"
FT /evidence="ECO:0000269|PubMed:1317314,
FT ECO:0000269|PubMed:14660699, ECO:0000269|PubMed:3224808"
SQ SEQUENCE 254 AA; 27605 MW; DE52DEA5DA7508D2 CRC64;
MAIANKNIIF VAGLGGIGFD TSREIVKRGP KNLVILDRIE NPAAIAELKA LNPKVTVTFY
PYDVTVSVAE TTKLLKTIFD KLKTVDLLIN GAGILDDHQI ERTIAVNFTG TVNTITAIMS
FWDKRKGGPG GVIANVCSVT GFNAIYQVPV YSASKAAALS FTNSLARLAP ITGVTAYSIN
PGITKTTLVH KFNSWLDVEP RVAELLLEHP TQTTLQCAQN FVKAIEANQN GAIWKVDLGT
LEAIEWTKHW DSHI
