DTFA_DICDI
ID DTFA_DICDI Reviewed; 1402 AA.
AC O96668; Q54HK7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Defective in tip formation protein A;
DE AltName: Full=Cell surface protein dtfA;
GN Name=dtfA; ORFNames=DDB_G0289389;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND REPEAT.
RC STRAIN=AX2;
RX PubMed=9693138; DOI=10.1242/dev.125.17.3343;
RA Ginger R.S., Drury L., Baader C., Zhukovskaya N.V., Williams J.G.;
RT "A novel Dictyostelium cell surface protein important for both cell
RT adhesion and cell sorting.";
RL Development 125:3343-3352(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Required for correct organization of the actin cytoskeleton
CC and cytokinesis. Also required for apical sorting of prestalk cells, a
CC prerequisite for formation of the tip at the mound stage and subsequent
CC formation of the fruiting body. May be required for cell adhesion.
CC {ECO:0000269|PubMed:9693138}.
CC -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000269|PubMed:9693138}.
CC Note=Forms a discrete lattice like arrangement at the cell surface.
CC -!- DEVELOPMENTAL STAGE: Expressed from the tight mound stage. Also
CC expressed in prespore and prestalk cells. {ECO:0000269|PubMed:9693138}.
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DR EMBL; AF102575; AAC98306.1; -; Genomic_DNA.
DR EMBL; AAFI02000140; EAL62722.1; -; Genomic_DNA.
DR PIR; T17456; T17456.
DR RefSeq; XP_636226.1; XM_631134.1.
DR AlphaFoldDB; O96668; -.
DR SMR; O96668; -.
DR STRING; 44689.DDB0191273; -.
DR PaxDb; O96668; -.
DR PRIDE; O96668; -.
DR EnsemblProtists; EAL62722; EAL62722; DDB_G0289389.
DR GeneID; 8627114; -.
DR KEGG; ddi:DDB_G0289389; -.
DR dictyBase; DDB_G0289389; dtfA.
DR HOGENOM; CLU_254260_0_0_1; -.
DR PhylomeDB; O96668; -.
DR PRO; PR:O96668; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0009986; C:cell surface; IDA:dictyBase.
DR GO; GO:0098609; P:cell-cell adhesion; IMP:dictyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
PE 2: Evidence at transcript level;
KW Cell adhesion; Cell cycle; Cell division; Coiled coil;
KW Developmental protein; Fruiting body; Reference proteome; Repeat.
FT CHAIN 1..1402
FT /note="Defective in tip formation protein A"
FT /id="PRO_0000327965"
FT REPEAT 12..18
FT /note="1"
FT /evidence="ECO:0000269|PubMed:9693138"
FT REPEAT 40..46
FT /note="2"
FT /evidence="ECO:0000269|PubMed:9693138"
FT REPEAT 60..66
FT /note="3"
FT /evidence="ECO:0000269|PubMed:9693138"
FT REPEAT 86..92
FT /note="4"
FT /evidence="ECO:0000269|PubMed:9693138"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 12..92
FT /note="4 X 7 AA repeat of T-T-T-[IV]-[AQ]-P-P"
FT REGION 37..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 109..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 429..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 712..745
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 350..383
FT /evidence="ECO:0000255"
FT COMPBIAS 47..64
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..94
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..239
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..256
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..282
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 191
FT /note="N -> I (in Ref. 1; AAC98306)"
FT /evidence="ECO:0000305"
FT CONFLICT 471
FT /note="F -> Y (in Ref. 1; AAC98306)"
FT /evidence="ECO:0000305"
FT CONFLICT 825
FT /note="K -> E (in Ref. 1; AAC98306)"
FT /evidence="ECO:0000305"
FT CONFLICT 828
FT /note="K -> E (in Ref. 1; AAC98306)"
FT /evidence="ECO:0000305"
FT CONFLICT 838
FT /note="K -> Q (in Ref. 1; AAC98306)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1402 AA; 164017 MW; 05A17C292E8F2306 CRC64;
MKDIEASKKP HTTTVAPPQI NFIKNNENIF QPKPISNVTT TTVQPPQIVS PPSPPSPPQT
TTIAPPTILP TTKTTTTTTT TTTTTTTVQP PQIVSPPIIN NLIIQNNLNT PSLSSTPSPL
PNNNNSNEND NDINNLKISK EEYQTQIEIQ QQIQRQQILE RQQLLQRRNQ EQLDLLERHN
DYQELINTHQ NFVPPNNRFS QQIHVSQLKK QSSQSQLQQQ LSSQSLQQIQ QKSKQPPPQQ
QQQQQPPPPP IPLLPQIHQQ LKPKQQQEQQ QQQEQQQQQT ENERINELRR LKRKKEYHND
EYKDDEIYLD NILKGIDIRK LEKLTAVELR KIGKTLGVPM GNNTKGETFQ RIKSFIENHK
KKKQKYREYQ SEKNQQQKSN SKKLVNNSTI YDLPIKDIEH LEQLFWRIFR NIVLFRKIIG
NLSRGGFFDQ QQQQQQQQQS TMTTTSSSSS PMTSSDDKFF YCYNYIFSKT FKYDQIIHVS
WIVDSNYFGL LKYKVSRGDL LVFCNHNTIN DGDVNDDEDQ NTKYEFCKMF KKIFNSIRSI
QDKSFYRDLF TNYSEFIFDR INKRYPIDIY LVGSLAIECN CLVATKLLIS EFQFRPVMNH
SLQLAIKSGS YKMVKLIVTT IHRQMSLNPN NKPFDIEMFS KINNPSIKII NLLIQLRLFT
YSNIINLVIA DAKLINDNNE NDILQFNKNL KNQIFNYSNL LNSFTFNNNI NNNNNNNINN
NNNNNNNNNN NNNNNNNNNN SNLSNNTIEY SKIKTKTVFN QFTIKQLINS CKLIVTFDLK
NQYQQQYYKR HYEDSDNEEE EEITQASFTI NTMAEIEFIE TQITKEEKHC FVKKVIQKQL
LKDGEKEDYD GIKRLAELYV SLNPHLKVYC NFMYKIIYGN ENCYDQDLDD EDLFDYRVTR
VFDSNCFKQS LKYGSPGYWI EYKEVEMKYA FLSNKYRNEI TPNLLFKYVS PNNLNKQLKF
IKKIYNSSIE NGNSNGNGNG NGGTVIGGIL DRLLLFYLII ENNNLELLSI VIKEFPLISN
FCYIAKSNSE IYNLKIPRFI RSIEMLEFCF SNFRDHFYLP QSNSLTADFY GFQNVELLRK
YDQLMVLDDM DKGIIVSNKK VRASFDDFHF IFEWGSSLKN YNNYLKMLAY IVEDPYGLYT
IVTNEILLLS AILSPTTSSG EPLLNLNIER QLIFQEIISS FSTILEGTEL KYYPEQMFQE
STHLKRFFDW IFENRSEDLL IGGRCVITQS VQSHMLYRAG RLDIVLRKGY YYDDTNEGSK
KIMPVGLALV LDDIGKYGDV VALEKYIRSC IPLMKQQSEL LTLLDKDERE SRACFSQCQR
HFSSLLSKAS IYGRINIFQH IFYNHQFIFD KKSLLFARKG FLSKISFKKL ILECHYHKQH
HILDFIQNVI GLDITPKQIK SN
