ID   DTML1_DICDI             Reviewed;         592 AA.
AC   Q54J86;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Dictomallein-1;
DE            EC=3.4.24.-;
DE   Flags: Precursor;
GN   Name=dtmlA; ORFNames=DDB_G0288219;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the dictomallein family. {ECO:0000305}.
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DR   EMBL; AAFI02000109; EAL63340.1; -; Genomic_DNA.
DR   RefSeq; XP_636850.1; XM_631758.1.
DR   AlphaFoldDB; Q54J86; -.
DR   SMR; Q54J86; -.
DR   PaxDb; Q54J86; -.
DR   EnsemblProtists; EAL63340; EAL63340; DDB_G0288219.
DR   GeneID; 8626518; -.
DR   KEGG; ddi:DDB_G0288219; -.
DR   dictyBase; DDB_G0288219; -.
DR   eggNOG; ENOG502SN3T; Eukaryota.
DR   HOGENOM; CLU_451780_0_0_1; -.
DR   InParanoid; Q54J86; -.
DR   PhylomeDB; Q54J86; -.
DR   PRO; PR:Q54J86; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR019503; Peptidase_M66_dom.
DR   PROSITE; PS51694; PEPTIDASE_M66; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW   Secreted; Signal; Zinc.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..592
FT                   /note="Dictomallein-1"
FT                   /id="PRO_0000322646"
FT   DOMAIN          140..402
FT                   /note="Peptidase M66"
FT   ACT_SITE        295
FT                   /evidence="ECO:0000250"
FT   BINDING         294
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         298
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         304
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   592 AA;  65793 MW;  D256499FDB53A2AA CRC64;
     MKILIILLVF LNLITNINCA VCSSRLQVSD IKFANTHVLP IEGKSWKNNT VTLKILPNRE
     SLLLAKFQDQ TLSYTVKVWV DDSLMGKLTL NDPSKLPPTE SNGTKYSTVH HSIRIPKDWM
     KPKMKIQFST MLLKSEFFFP NIGHETNLNM WLLPFYLFGA NDTNTQPLSV TGSISKDVSD
     ELIQKWSLSS LNALNHPISR IDWSYLILPA GRNNLPGLRI TNSDQKRDGY EIMSVVLGIL
     SGIRGANGEG PSSVLYYAPL IHLGANGKYA DPWGGLGGGS VGTGDYSYTG IFIHEAGHSY
     GLPHAGDSYK SGNYPYVDGS LLGSEWGFDM NHNEFLSVNI PSNIGAYKNC NKSFILDQNK
     NCVKQSVMQG GAGNQAQGYR YSMFADYEEV TIQNYFKDSI IYDKSFSTGY KKWNTTTLKY
     ETYTPSTKSN GLYGINDGLP IERNVDVYTI IITHSFVGPA NLSQIYPIFK SKGNLMPFFD
     PTNSTQLVDI RPNVSKYAWY CHANGCDYTI KITYTDGTTK YMLLQRGKRS WFSPSGAIGS
     GFTDPLSGES SLSVIFNIKA DKTPSKIELF ETLLGFNGFN STTATLLVSQ SF