ID   DTML2_DICDI             Reviewed;         599 AA.
AC   Q54CQ0;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Dictomallein-2;
DE            EC=3.4.24.-;
DE   Flags: Precursor;
GN   Name=dtmlB; ORFNames=DDB_G0292822;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the dictomallein family. {ECO:0000305}.
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DR   EMBL; AAFI02000197; EAL60960.1; -; Genomic_DNA.
DR   RefSeq; XP_629366.1; XM_629364.1.
DR   AlphaFoldDB; Q54CQ0; -.
DR   STRING; 44689.DDB0252868; -.
DR   PaxDb; Q54CQ0; -.
DR   EnsemblProtists; EAL60960; EAL60960; DDB_G0292822.
DR   GeneID; 8628883; -.
DR   KEGG; ddi:DDB_G0292822; -.
DR   dictyBase; DDB_G0292822; -.
DR   eggNOG; ENOG502SN3T; Eukaryota.
DR   HOGENOM; CLU_451780_0_0_1; -.
DR   InParanoid; Q54CQ0; -.
DR   OMA; HAGEAYD; -.
DR   PhylomeDB; Q54CQ0; -.
DR   PRO; PR:Q54CQ0; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR019503; Peptidase_M66_dom.
DR   PROSITE; PS51694; PEPTIDASE_M66; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW   Secreted; Signal; Zinc.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..599
FT                   /note="Dictomallein-2"
FT                   /id="PRO_0000322647"
FT   DOMAIN          145..407
FT                   /note="Peptidase M66"
FT   ACT_SITE        299
FT                   /evidence="ECO:0000250"
FT   BINDING         298
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         302
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         308
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   599 AA;  67712 MW;  F35FD52A67249CDC CRC64;
     MKLILIYLIL VFNLFNFINC QNILKVSNVR FAQTHVIPIE GKSWNIQGST KHMSIVGKRR
     ALLLASFQDQ NLSYFATIWY DGGKVGMIQL NDPSQLPLTE DNGEKYSKVH HSGMIPKEWV
     RVGMKIQFSS FGGINGTEVS DILSPDVGQD YTLKMWILPF YLFGANDTNT QPFSKTKGID
     SGISKELIEK WSCSDLQADN HPIQKIDWPY FVMEPRSGNP AMVITNSDQK KDGYAIMNGV
     LSILWLLRGM FGESSSSIQI YSPLLHLDAK GRYADTYGGL GGSSAGTGNH RFTGIFIHEQ
     GHAMGLPHAG EAYDNKRKYP YKQGSLSGSE WGFDANHNEF LGTFIPPTAE LYKTCKKNSI
     IDSKGRCVKQ SVMQSGAGDQ SSKYRYSMFA DFEMTTIQNY FKNSIYYDET NGKYKKWNDT
     SKSYYAYKPI TKEKGFEGVD ENTPIERNVD IYSIIFTYST VEKPKVGKIS QIYPLLKSKG
     NLIRQFDPTN KKEMDSVNPK LNGEFKWYCN SSGCDYTIRV TFYDSSLKHV LLQQGKRKYK
     LPTGSFRDNI NDPKSSDSFM LGGVNIKANK KIKKVELLET PFAWNGIPKN PTVLVSKSF