ID   DTML4_DICDI             Reviewed;         594 AA.
AC   Q54CP8;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Dictomallein-4;
DE            EC=3.4.24.-;
DE   Flags: Precursor;
GN   Name=dtmlD; ORFNames=DDB_G0292826;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the dictomallein family. {ECO:0000305}.
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DR   EMBL; AAFI02000197; EAL60962.1; -; Genomic_DNA.
DR   RefSeq; XP_629368.1; XM_629366.1.
DR   AlphaFoldDB; Q54CP8; -.
DR   STRING; 44689.DDB0252870; -.
DR   PaxDb; Q54CP8; -.
DR   EnsemblProtists; EAL60962; EAL60962; DDB_G0292826.
DR   GeneID; 8628885; -.
DR   KEGG; ddi:DDB_G0292826; -.
DR   dictyBase; DDB_G0292826; -.
DR   eggNOG; ENOG502SN3T; Eukaryota.
DR   HOGENOM; CLU_451780_0_0_1; -.
DR   InParanoid; Q54CP8; -.
DR   PhylomeDB; Q54CP8; -.
DR   PRO; PR:Q54CP8; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR019503; Peptidase_M66_dom.
DR   PROSITE; PS51694; PEPTIDASE_M66; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW   Secreted; Signal; Zinc.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..594
FT                   /note="Dictomallein-4"
FT                   /id="PRO_0000322649"
FT   DOMAIN          147..408
FT                   /note="Peptidase M66"
FT   ACT_SITE        301
FT                   /evidence="ECO:0000250"
FT   BINDING         300
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         304
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         310
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   594 AA;  67369 MW;  E4CD7C4EB2420425 CRC64;
     MKLVLIFLII NFLLIINCEN LIKVLDVRLA QTHIIPIEGK TWNLKNKTQH MSIIGNRRAL
     LLASFEDLNK SYYVSIWLDN KIIGSLELKD PSQLPQTEDN GERYSTKHHS VLLPKEWIRP
     NMKIKFTLTE SKDNSSLNID SSNFFYPDVS QDYTLKMWTL PFYLFGANDT NTQPFSVTNG
     IGSEITKELM ERWSCSDIIA VNHPIQRIDW PYLVMEPRNG NPAMLITNSD QKKDGYAIMN
     GVLNILTEIR EAFGESTSSI QIYSPLLHLG ANGKYTNPGG GLGGGSRGTG DHKYTFTFFH
     EQGHAMGLPH AGEAFAAGSY PYVNGSLLGS EWGYDANHNE LLGTFIPPTS DQFKNCRRNS
     VFDSKGRCVK QSVMQGGAGD YSSKYRYSMF ADFEMTTIQN YFKNSIYYDQ TKGTYKKWND
     TSKSYFEYIP STKNNGLWGL DDGTPIERDI EVYTILFTYS TVGPKELSQI YPILKSSKGN
     LMKRYDPTNK NDMKLITPGS GSWYCFASGC DYTVRVTFDD DSLEHILLRQ GKRKYWNPMG
     DFKENLYNAT SSNSFILRGI NVKATKSIKK VELLETLMAW KGISNATVLV SKDF