ID   DTML5_DICDI             Reviewed;         623 AA.
AC   Q54CP7;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Dictomallein-5;
DE            EC=3.4.24.-;
DE   Flags: Precursor;
GN   Name=dtmlE; ORFNames=DDB_G0292828;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the dictomallein family. {ECO:0000305}.
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DR   EMBL; AAFI02000197; EAL60963.1; -; Genomic_DNA.
DR   RefSeq; XP_629369.1; XM_629367.1.
DR   AlphaFoldDB; Q54CP7; -.
DR   STRING; 44689.DDB0252838; -.
DR   PaxDb; Q54CP7; -.
DR   EnsemblProtists; EAL60963; EAL60963; DDB_G0292828.
DR   GeneID; 8628886; -.
DR   KEGG; ddi:DDB_G0292828; -.
DR   dictyBase; DDB_G0292828; -.
DR   eggNOG; ENOG502SN3T; Eukaryota.
DR   HOGENOM; CLU_451780_0_0_1; -.
DR   InParanoid; Q54CP7; -.
DR   OMA; ANEEWIQ; -.
DR   PhylomeDB; Q54CP7; -.
DR   PRO; PR:Q54CP7; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.390.10; -; 1.
DR   InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR   InterPro; IPR019503; Peptidase_M66_dom.
DR   PROSITE; PS51694; PEPTIDASE_M66; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW   Secreted; Signal; Zinc.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..623
FT                   /note="Dictomallein-5"
FT                   /id="PRO_0000322650"
FT   DOMAIN          174..435
FT                   /note="Peptidase M66"
FT   ACT_SITE        328
FT                   /evidence="ECO:0000250"
FT   BINDING         327
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         331
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         337
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   623 AA;  70749 MW;  F79D5F5DA1A3872B CRC64;
     MKIFIIKIIL VLFNYVLLSY SINQDLNILN VYDVRFAQTH VIPIEGKSWI LNNKTYTLTI
     VGNRDSLLLV KFIDISITGT SSIKTVTTKT TKEEIKATIL TNTYNVMVWN NDIVMGTRLL
     NDPNQLPPTE SNGEQYSNEH HSIMIPKEWV KPGMKLQFFE NSNSFIKSSK FIFPNVGQDY
     TLKMWTLPFY LFGANDTNTR PFNQTKNIDD SISAGLSQVY SCSNILSLNH PIQRVDWPYL
     VLGPRNGLPG MLITNSDQKK DGYAIMEGVL NILTGIRVAF GESTSSIQIY APLLHLGANG
     KYADTYGGLG GSSRGTGDYR YSDTFVHEQG HAMGLPHAGE AFASGSYPYV NGSLLGSEWG
     FDINHYEFLS ITLSNTSKNY KGCEKKNVKD TQNRCVKQSV MQSGAGDRSS NYLFSMFSDF
     EMSIIQNYFK NSIYYDEQIG KYKKWNETIG SYYEYKPITK DYGFYRLDDG IPIERDIDVY
     TIVFTYSLVG PEPLSQIYPL LKSKGNLIRQ FDPTNKTEMK LITPNTGSIP WYCFNSGCDY
     TIRVTYDDDS IKHILLQQGK RQYWKPMGDF KLNFNDPTSS DSFLLGVINV KAIKTIKKVE
     LLETLMAWNG ISKNSKLLTF KLF