ADH2_ECOLI
ID ADH2_ECOLI Reviewed; 383 AA.
AC P37686; Q2M7Q2;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 4.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Probable alcohol dehydrogenase;
DE EC=1.1.1.1;
GN Name=yiaY; OrderedLocusNames=b3589, JW5648;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP SEQUENCE REVISION TO 380-383.
RX PubMed=16397293; DOI=10.1093/nar/gkj405;
RA Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA Thomson N.R., Wishart D., Wanner B.L.;
RT "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT -- 2005.";
RL Nucleic Acids Res. 34:1-9(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 336-383.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7768815; DOI=10.1128/jb.177.11.3166-3175.1995;
RA Xu J., Johnson R.C.;
RT "aldB, an RpoS-dependent gene in Escherichia coli encoding an aldehyde
RT dehydrogenase that is repressed by Fis and activated by Crp.";
RL J. Bacteriol. 177:3166-3175(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC family. {ECO:0000305}.
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DR EMBL; U00039; AAB18566.1; -; Genomic_DNA.
DR EMBL; U00096; AAT48195.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77704.1; -; Genomic_DNA.
DR EMBL; L40742; AAC36938.1; -; Genomic_DNA.
DR PIR; S47810; S47810.
DR RefSeq; WP_000741518.1; NZ_SSZK01000041.1.
DR RefSeq; YP_026233.1; NC_000913.3.
DR AlphaFoldDB; P37686; -.
DR SMR; P37686; -.
DR BioGRID; 4262556; 14.
DR DIP; DIP-12414N; -.
DR STRING; 511145.b3589; -.
DR PaxDb; P37686; -.
DR PRIDE; P37686; -.
DR EnsemblBacteria; AAT48195; AAT48195; b3589.
DR EnsemblBacteria; BAE77704; BAE77704; BAE77704.
DR GeneID; 67417592; -.
DR GeneID; 948102; -.
DR KEGG; ecj:JW5648; -.
DR KEGG; eco:b3589; -.
DR PATRIC; fig|1411691.4.peg.3122; -.
DR EchoBASE; EB2201; -.
DR eggNOG; COG1454; Bacteria.
DR HOGENOM; CLU_007207_0_0_6; -.
DR InParanoid; P37686; -.
DR OMA; HAMSHQV; -.
DR PhylomeDB; P37686; -.
DR BioCyc; EcoCyc:EG12293-MON; -.
DR BioCyc; MetaCyc:EG12293-MON; -.
DR PRO; PR:P37686; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IMP:CACAO.
DR GO; GO:0008743; F:L-threonine 3-dehydrogenase activity; IDA:EcoCyc.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR InterPro; IPR001670; ADH_Fe/GldA.
DR InterPro; IPR018211; ADH_Fe_CS.
DR InterPro; IPR039697; Alcohol_dehydrogenase_Fe.
DR PANTHER; PTHR11496; PTHR11496; 1.
DR Pfam; PF00465; Fe-ADH; 1.
DR PROSITE; PS00913; ADH_IRON_1; 1.
DR PROSITE; PS00060; ADH_IRON_2; 1.
PE 3: Inferred from homology;
KW Iron; NAD; Oxidoreductase; Reference proteome.
FT CHAIN 1..383
FT /note="Probable alcohol dehydrogenase"
FT /id="PRO_0000087816"
FT CONFLICT 380..383
FT /note="RAAM -> AAR (in Ref. 1; AAB18566)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 383 AA; 40351 MW; 805DC4E488E108F6 CRC64;
MAASTFFIPS VNVIGADSLT DAMNMMADYG FTRTLIVTDN MLTKLGMAGD VQKALEERNI
FSVIYDGTQP NPTTENVAAG LKLLKENNCD SVISLGGGSP HDCAKGIALV AANGGDIRDY
EGVDRSAKPQ LPMIAINTTA GTASEMTRFC IITDEARHIK MAIVDKHVTP LLSVNDSSLM
IGMPKSLTAA TGMDALTHAI EAYVSIAATP ITDACALKAV TMIAENLPLA VEDGSNAKAR
EAMAYAQFLA GMAFNNASLG YVHAMAHQLG GFYNLPHGVC NAVLLPHVQV FNSKVAAARL
RDCAAAMGVN VTGKNDAEGA EACINAIREL AKKVDIPAGL RDLNVKEEDF AVLATNALKD
ACGFTNPIQA THEEIVAIYR AAM
