ID   DTWD1_DANRE             Reviewed;         251 AA.
AC   Q5XJ56;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=tRNA-uridine aminocarboxypropyltransferase 1 {ECO:0000305};
DE            EC=2.5.1.25 {ECO:0000250|UniProtKB:Q8N5C7};
DE   AltName: Full=DTW domain-containing protein 1 {ECO:0000305};
GN   Name=dtwd1 {ECO:0000250|UniProtKB:Q8N5C7};
GN   ORFNames=zgc:10363 {ECO:0000303|Ref.1};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Larva;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine
CC       (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs
CC       (acp3U(20)). {ECO:0000250|UniProtKB:Q8N5C7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC         amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC         thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC         COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC         Evidence={ECO:0000250|UniProtKB:Q8N5C7};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8N5C7}.
CC   -!- DOMAIN: Contains 1 DXTW motif.
CC   -!- SIMILARITY: Belongs to the TDD superfamily. DTWD1 family.
CC       {ECO:0000305}.
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DR   EMBL; BC083451; AAH83451.1; -; mRNA.
DR   AlphaFoldDB; Q5XJ56; -.
DR   STRING; 7955.ENSDARP00000076931; -.
DR   PaxDb; Q5XJ56; -.
DR   ZFIN; ZDB-GENE-041010-23; dtwd1.
DR   eggNOG; KOG3795; Eukaryota.
DR   InParanoid; Q5XJ56; -.
DR   PhylomeDB; Q5XJ56; -.
DR   PRO; PR:Q5XJ56; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR   InterPro; IPR005636; DTW.
DR   Pfam; PF03942; DTW; 1.
DR   SMART; SM01144; DTW; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; S-adenosyl-L-methionine; Transferase;
KW   tRNA processing.
FT   CHAIN           1..251
FT                   /note="tRNA-uridine aminocarboxypropyltransferase 1"
FT                   /id="PRO_0000308216"
FT   MOTIF           160..163
FT                   /note="DXTW"
SQ   SEQUENCE   251 AA;  28429 MW;  8D37615B07E60509 CRC64;
     MSEDPQPESS ALSDLKIASH APLDDAQRAG RMKCTRCGCS RMFFCYSCGA LVGLQPGDVP
     RVTLPVKIDI IKHPNETDGK STAVQAKLLA PQDVTIYTYP CIPELDQSAE NIVLVFPGPD
     AMSVEELWEY FCADGRPRVK RVKAEAESLR CPIQRVVFID STWNQTSRII TDERLQALPN
     VELKSRKTCF WRRQKGSPDT YLATIEAIYY FLKDLHCHYF CEYTGEYDNL LFFFSFLHKL
     INKAKQAAGK A