DTWD1_MOUSE
ID DTWD1_MOUSE Reviewed; 304 AA.
AC Q9D8U7;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=tRNA-uridine aminocarboxypropyltransferase 1 {ECO:0000305};
DE EC=2.5.1.25 {ECO:0000250|UniProtKB:Q8N5C7};
DE AltName: Full=DTW domain-containing protein 1 {ECO:0000305};
GN Name=Dtwd1 {ECO:0000312|MGI:MGI:1916435};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine
CC (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs
CC (acp3U(20)). {ECO:0000250|UniProtKB:Q8N5C7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC Evidence={ECO:0000250|UniProtKB:Q8N5C7};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8N5C7}.
CC -!- DOMAIN: Contains 1 DXTW motif.
CC -!- SIMILARITY: Belongs to the TDD superfamily. DTWD1 family.
CC {ECO:0000305}.
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DR EMBL; AK007678; BAB25182.1; -; mRNA.
DR EMBL; AL929175; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC020149; AAH20149.1; -; mRNA.
DR CCDS; CCDS16682.1; -.
DR RefSeq; NP_081257.1; NM_026981.2.
DR RefSeq; XP_006500192.1; XM_006500129.1.
DR RefSeq; XP_006500193.1; XM_006500130.1.
DR RefSeq; XP_006500194.1; XM_006500131.1.
DR AlphaFoldDB; Q9D8U7; -.
DR STRING; 10090.ENSMUSP00000127662; -.
DR iPTMnet; Q9D8U7; -.
DR PhosphoSitePlus; Q9D8U7; -.
DR EPD; Q9D8U7; -.
DR MaxQB; Q9D8U7; -.
DR PaxDb; Q9D8U7; -.
DR PRIDE; Q9D8U7; -.
DR ProteomicsDB; 277632; -.
DR Antibodypedia; 65167; 156 antibodies from 20 providers.
DR DNASU; 69185; -.
DR Ensembl; ENSMUST00000110437; ENSMUSP00000106067; ENSMUSG00000023330.
DR Ensembl; ENSMUST00000170908; ENSMUSP00000127662; ENSMUSG00000023330.
DR GeneID; 69185; -.
DR KEGG; mmu:69185; -.
DR UCSC; uc008mdk.2; mouse.
DR CTD; 56986; -.
DR MGI; MGI:1916435; Dtwd1.
DR VEuPathDB; HostDB:ENSMUSG00000023330; -.
DR eggNOG; KOG3795; Eukaryota.
DR GeneTree; ENSGT00940000153766; -.
DR HOGENOM; CLU_069451_0_0_1; -.
DR InParanoid; Q9D8U7; -.
DR OMA; VNAWGLN; -.
DR OrthoDB; 824721at2759; -.
DR PhylomeDB; Q9D8U7; -.
DR TreeFam; TF324733; -.
DR BioGRID-ORCS; 69185; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q9D8U7; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9D8U7; protein.
DR Bgee; ENSMUSG00000023330; Expressed in spermatocyte and 221 other tissues.
DR Genevisible; Q9D8U7; MM.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; ISS:UniProtKB.
DR GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR InterPro; IPR005636; DTW.
DR Pfam; PF03942; DTW; 1.
DR SMART; SM01144; DTW; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome; S-adenosyl-L-methionine; Transferase;
KW tRNA processing.
FT CHAIN 1..304
FT /note="tRNA-uridine aminocarboxypropyltransferase 1"
FT /id="PRO_0000308213"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 206..209
FT /note="DXTW"
SQ SEQUENCE 304 AA; 34734 MW; 13E7E8258AE47137 CRC64;
MALSPSVVPQ ESKEDNANCV ETKPSQTTSI ASEDPLQNLC LASQEVLRKA QQSGRSRCRQ
CGGSRMFYCY TCCVPVGNVP TEQIPCVQLP LKIDIIKHPN ETDGKSTAVH AKLLAPDSVN
IYTYPCIPEY EGKDHEVVLV FPGPQSISIE DVSFHLQKRI ESKGRNKADN LDVPPRKLKR
TTDEEGWDLH ESTRQGPELK RVVFIDSTWS QTNQIASDER LRELLQVELR TRKTCFWRHQ
KGKPDTFLST IEAIYYFLVD YHSAVQKEKY RGQYDNLLFF YSFMYRLIKN ARGSGEKAKP
QLVQ
