ID   DTWD1_RAT               Reviewed;         304 AA.
AC   Q6AYF5;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=tRNA-uridine aminocarboxypropyltransferase 1 {ECO:0000305};
DE            EC=2.5.1.25 {ECO:0000250|UniProtKB:Q8N5C7};
DE   AltName: Full=DTW domain-containing protein 1 {ECO:0000305};
GN   Name=Dtwd1 {ECO:0000312|RGD:1311144};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine
CC       (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs
CC       (acp3U(20)). {ECO:0000250|UniProtKB:Q8N5C7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC         amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC         thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC         COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC         Evidence={ECO:0000250|UniProtKB:Q8N5C7};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8N5C7}.
CC   -!- DOMAIN: Contains 1 DXTW motif.
CC   -!- SIMILARITY: Belongs to the TDD superfamily. DTWD1 family.
CC       {ECO:0000305}.
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DR   EMBL; BC079068; AAH79068.1; -; mRNA.
DR   RefSeq; NP_001013943.1; NM_001013921.1.
DR   AlphaFoldDB; Q6AYF5; -.
DR   STRING; 10116.ENSRNOP00000012966; -.
DR   PaxDb; Q6AYF5; -.
DR   PRIDE; Q6AYF5; -.
DR   GeneID; 296119; -.
DR   KEGG; rno:296119; -.
DR   UCSC; RGD:1311144; rat.
DR   CTD; 56986; -.
DR   RGD; 1311144; Dtwd1.
DR   eggNOG; KOG3795; Eukaryota.
DR   InParanoid; Q6AYF5; -.
DR   OrthoDB; 824721at2759; -.
DR   PhylomeDB; Q6AYF5; -.
DR   PRO; PR:Q6AYF5; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR   InterPro; IPR005636; DTW.
DR   Pfam; PF03942; DTW; 1.
DR   SMART; SM01144; DTW; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; S-adenosyl-L-methionine; Transferase;
KW   tRNA processing.
FT   CHAIN           1..304
FT                   /note="tRNA-uridine aminocarboxypropyltransferase 1"
FT                   /id="PRO_0000308215"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           206..209
FT                   /note="DXTW"
SQ   SEQUENCE   304 AA;  34903 MW;  E329143A5C7B1CB9 CRC64;
     MALSPSVVPQ ESEENNANCV ETKQSQTAST ASEDPLQHLC LASQEVLHKA QQSGRSRCLQ
     CGGSRMFYCY TCYVPVENVP TEQIPFVQLP LKIDIIKHPN ETDGKSTAVH AKLLAPDSVN
     IYTYPCIPEY EGQDHEVVLV FPGPQSISIK DVSFHLQKRI ESKGGDKADD LDMPPRKLVR
     TEAQEGWHLN ESMGKGPELK RVVFIDSTWS QTNQITSDER LRELLQVELK TRKTCFWRHQ
     KGKPDTFLST IEAIYYFLVD YHRAVQKEEY RGQYDNLLFF YSFMYRLIKE ARRSGEKAKQ
     KPIH