ID   DTWD1_XENLA             Reviewed;         291 AA.
AC   Q6DDV1;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=tRNA-uridine aminocarboxypropyltransferase 1 {ECO:0000305};
DE            EC=2.5.1.25 {ECO:0000250|UniProtKB:Q8N5C7};
DE   AltName: Full=DTW domain-containing protein 1 {ECO:0000305};
GN   Name=dtwd1 {ECO:0000250|UniProtKB:Q8N5C7};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine
CC       (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs
CC       (acp3U(20)). {ECO:0000250|UniProtKB:Q8N5C7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC         amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC         thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC         COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC         Evidence={ECO:0000250|UniProtKB:Q8N5C7};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8N5C7}.
CC   -!- DOMAIN: Contains 1 DXTW motif.
CC   -!- SIMILARITY: Belongs to the TDD superfamily. DTWD1 family.
CC       {ECO:0000305}.
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DR   EMBL; BC077405; AAH77405.1; -; mRNA.
DR   RefSeq; NP_001086758.1; NM_001093289.1.
DR   RefSeq; XP_018106510.1; XM_018251021.1.
DR   RefSeq; XP_018106511.1; XM_018251022.1.
DR   RefSeq; XP_018106512.1; XM_018251023.1.
DR   AlphaFoldDB; Q6DDV1; -.
DR   MaxQB; Q6DDV1; -.
DR   DNASU; 446593; -.
DR   GeneID; 446593; -.
DR   KEGG; xla:446593; -.
DR   CTD; 446593; -.
DR   Xenbase; XB-GENE-944647; dtwd1.L.
DR   OMA; VNAWGLN; -.
DR   OrthoDB; 824721at2759; -.
DR   Proteomes; UP000186698; Chromosome 3L.
DR   Bgee; 446593; Expressed in gastrula and 19 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR   InterPro; IPR005636; DTW.
DR   Pfam; PF03942; DTW; 1.
DR   SMART; SM01144; DTW; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; S-adenosyl-L-methionine; Transferase;
KW   tRNA processing.
FT   CHAIN           1..291
FT                   /note="tRNA-uridine aminocarboxypropyltransferase 1"
FT                   /id="PRO_0000308217"
FT   REGION          158..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           199..202
FT                   /note="DXTW"
FT   COMPBIAS        161..181
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   291 AA;  33542 MW;  4BBE4AA6A8B2FB62 CRC64;
     MSHEKSLQEK KDSSSFKSFH DGASLENDLL QNLQLSSQTV LEIAQKKGRS KCPKCNSSRM
     FYCYTCFVPV ESVPSDDIPV VKLPLKIDII KHPNETDGKS TAVHAKLLAH EDVTVYTYPC
     VPQYHDQKHE VVVVFPGPDS VSLSDYLLYL SSSGDLEKNS AYEPSSKRPK FSPENDKNTY
     EGIDKKKPVN FLKKVIFIDS TWNQTNKMIA DERLQGLVHV ELMERKTFFW RHQKGTPNTY
     LSTIEAIYYF MVDYHTKILQ KDYKGEYDNL LFFFSFMYRI INDAKKSAGK L