ID   DTWD1_XENTR             Reviewed;         294 AA.
AC   Q28I29;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=tRNA-uridine aminocarboxypropyltransferase 1 {ECO:0000305};
DE            EC=2.5.1.25 {ECO:0000250|UniProtKB:Q8N5C7};
DE   AltName: Full=DTW domain-containing protein 1 {ECO:0000305};
GN   Name=dtwd1 {ECO:0000250|UniProtKB:Q8N5C7};
GN   ORFNames=TEgg073n17.1 {ECO:0000303|Ref.1};
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine
CC       (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs
CC       (acp3U(20)). {ECO:0000250|UniProtKB:Q8N5C7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC         amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC         thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC         COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC         Evidence={ECO:0000250|UniProtKB:Q8N5C7};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8N5C7}.
CC   -!- DOMAIN: Contains 1 DXTW motif.
CC   -!- SIMILARITY: Belongs to the TDD superfamily. DTWD1 family.
CC       {ECO:0000305}.
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DR   EMBL; CR760622; CAJ81336.1; -; mRNA.
DR   RefSeq; NP_001017188.1; NM_001017188.2.
DR   RefSeq; XP_017947601.1; XM_018092112.1.
DR   AlphaFoldDB; Q28I29; -.
DR   STRING; 8364.ENSXETP00000019301; -.
DR   PaxDb; Q28I29; -.
DR   GeneID; 549942; -.
DR   KEGG; xtr:549942; -.
DR   CTD; 56986; -.
DR   Xenbase; XB-GENE-944642; dtwd1.
DR   eggNOG; KOG3795; Eukaryota.
DR   HOGENOM; CLU_069451_0_0_1; -.
DR   InParanoid; Q28I29; -.
DR   OMA; YWRPQRG; -.
DR   OrthoDB; 824721at2759; -.
DR   PhylomeDB; Q28I29; -.
DR   TreeFam; TF324733; -.
DR   Proteomes; UP000008143; Chromosome 3.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR   InterPro; IPR005636; DTW.
DR   Pfam; PF03942; DTW; 1.
DR   SMART; SM01144; DTW; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; S-adenosyl-L-methionine; Transferase;
KW   tRNA processing.
FT   CHAIN           1..294
FT                   /note="tRNA-uridine aminocarboxypropyltransferase 1"
FT                   /id="PRO_0000308218"
FT   REGION          158..185
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           202..205
FT                   /note="DXTW"
SQ   SEQUENCE   294 AA;  34036 MW;  0CC75DFA76387C03 CRC64;
     MSHEKSESCL QEKKHSSSFE RFHDGTSLEN NLLQNLKLSS QRVLEIAQKK GRSKCPKCNS
     SRMFYCYTCF VPVESVPSDE IPVVKLPLKI DIIKHPNETD GKSTAVHAKL LAHEDVTVYT
     YPCVPQYQDQ KHEVVLVFPG PDSVSLSDSL LYIRGSGDMQ NDSSCEPSLK RPKCSQQYDK
     SKNEGVEEKK PMHFLKKLIF IDSTWNQTNK IISDERLQGL QQVELMERKT CFWRHQKGTP
     NTYLSTIEAI YYFMIDYHTI ILQKDYKGEY DDLLFFFSFM YRIINDAKKS AGKL