DTX3_MOUSE
ID DTX3_MOUSE Reviewed; 347 AA.
AC Q80V91; Q4FZD3; Q9ER06;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2003, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Probable E3 ubiquitin-protein ligase DTX3;
DE EC=2.3.2.27 {ECO:0000250|UniProtKB:Q61010};
DE AltName: Full=Protein deltex-3;
DE Short=Deltex3;
DE Short=mDTX3;
DE AltName: Full=RING-type E3 ubiquitin transferase DTX3 {ECO:0000305};
GN Name=Dtx3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP MULTIMERIZATION.
RX PubMed=11226752; DOI=10.1016/s0736-5748(00)00071-x;
RA Kishi N., Tang Z., Maeda Y., Hirai A., Mo R., Ito M., Suzuki S., Nakao K.,
RA Kinoshita T., Kadesch T., Hui C.-C., Artavanis-Tsakonas S., Okano H.,
RA Matsuno K.;
RT "Murine homologs of deltex define a novel gene family involved in
RT vertebrate Notch signaling and neurogenesis.";
RL Int. J. Dev. Neurosci. 19:21-35(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulator of Notch signaling, a signaling pathway involved in
CC cell-cell communications that regulates a broad spectrum of cell-fate
CC determinations. Probably acts both as a positive and negative regulator
CC of Notch, depending on the developmental and cell context. Functions as
CC a ubiquitin ligase protein in vitro, suggesting that it may regulate
CC the Notch pathway via some ubiquitin ligase activity. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000250|UniProtKB:Q61010};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Homodimer. May form a heterodimer with other members of the
CC Deltex family. Interacts with NOTCH1.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in testis and brain. Weakly
CC expressed in kidney. {ECO:0000269|PubMed:11226752}.
CC -!- DEVELOPMENTAL STAGE: In the CNS, it is expressed in the developing
CC neural tube starting from 10.5 dpc in the spinal cord and around 11.5
CC dpc in the telencephalon. Expressed ubiquitously throughout the spinal
CC cord and telencephalon during neurogenesis. Expressed throughout the
CC developing retina at 15.5 dpc. Not expressed in the somite or presomite
CC during somitogenesis. Expressed slightly later that Dtx2.
CC {ECO:0000269|PubMed:11226752}.
CC -!- SIMILARITY: Belongs to the Deltex family. {ECO:0000305}.
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DR EMBL; AB015425; BAB18942.1; -; mRNA.
DR EMBL; BC099687; AAH99687.1; -; mRNA.
DR EMBL; BC138304; AAI38305.1; -; mRNA.
DR EMBL; BC157899; AAI57900.1; -; mRNA.
DR CCDS; CCDS36078.1; -.
DR RefSeq; NP_109639.1; NM_030714.2.
DR AlphaFoldDB; Q80V91; -.
DR SMR; Q80V91; -.
DR STRING; 10090.ENSMUSP00000044627; -.
DR iPTMnet; Q80V91; -.
DR PhosphoSitePlus; Q80V91; -.
DR MaxQB; Q80V91; -.
DR PaxDb; Q80V91; -.
DR PRIDE; Q80V91; -.
DR ProteomicsDB; 279487; -.
DR Antibodypedia; 28813; 164 antibodies from 29 providers.
DR DNASU; 80904; -.
DR Ensembl; ENSMUST00000038217; ENSMUSP00000044627; ENSMUSG00000040415.
DR GeneID; 80904; -.
DR KEGG; mmu:80904; -.
DR UCSC; uc007hil.1; mouse.
DR CTD; 196403; -.
DR MGI; MGI:2135752; Dtx3.
DR VEuPathDB; HostDB:ENSMUSG00000040415; -.
DR eggNOG; ENOG502QUYA; Eukaryota.
DR GeneTree; ENSGT00940000161404; -.
DR HOGENOM; CLU_030422_1_0_1; -.
DR InParanoid; Q80V91; -.
DR OMA; PRTLERC; -.
DR OrthoDB; 143182at2759; -.
DR TreeFam; TF325526; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 80904; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Dtx3; mouse.
DR PRO; PR:Q80V91; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q80V91; protein.
DR Bgee; ENSMUSG00000040415; Expressed in retinal neural layer and 234 other tissues.
DR ExpressionAtlas; Q80V91; baseline and differential.
DR Genevisible; Q80V91; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0007219; P:Notch signaling pathway; IDA:MGI.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR CDD; cd09633; Deltex_C; 1.
DR Gene3D; 3.30.390.130; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR039396; Deltex_C.
DR InterPro; IPR039399; Deltex_C_sf.
DR InterPro; IPR039398; Deltex_fam.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR12622; PTHR12622; 1.
DR Pfam; PF18102; DTC; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Metal-binding; Notch signaling pathway; Reference proteome;
KW Transferase; Ubl conjugation pathway; Zinc; Zinc-finger.
FT CHAIN 1..347
FT /note="Probable E3 ubiquitin-protein ligase DTX3"
FT /id="PRO_0000219086"
FT ZN_FING 164..205
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 111..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..151
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 347 AA; 37982 MW; 2368BB96D49967AD CRC64;
MSFVLSRMAA CGGSCKNKVT VSKPVWDFLS KETPARLARL REEHRVSILI DGETSDIYVL
QLSPQGPPPA PPNGLYLARK ALKGLLKEAE KELKKAQRQG ELMGCLALGG GGEHPELHRP
GPPPLRAAPL LPPGARGLPP PPPPLPPPLP PRLREDAEEQ ETTCPICLGE IQNAKTLEKC
RHSFCEGCIT RALQVKKACP MCGRFYGQLV GNQPQNGRML VSKDATLLLP SYEKYGTIVI
QYVFPPGVQG AEHPNPGVRY PGTTRVAYLP DCPEGNKVLT LFRKAFDQRL TFTIGTSMTT
GRPNVITWND IHHKTSCTGG PQLFGYPDPT YLTRVQEELR AKGITDD
