DTX46_ARATH
ID DTX46_ARATH Reviewed; 559 AA.
AC Q8W4G3; Q8LDE3; Q9SJU3;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Protein DETOXIFICATION 46, chloroplastic {ECO:0000303|PubMed:11739388};
DE Short=AtDTX46 {ECO:0000303|PubMed:11739388};
DE AltName: Full=Multidrug and toxic compound extrusion protein 46 {ECO:0000305};
DE Short=MATE protein 46 {ECO:0000305};
DE AltName: Full=Protein EDS5 HOMOLOGUE {ECO:0000303|PubMed:26055508};
DE Flags: Precursor;
GN Name=DTX46 {ECO:0000303|PubMed:11739388};
GN Synonyms=EDS5H {ECO:0000303|PubMed:26055508};
GN OrderedLocusNames=At2g21340 {ECO:0000312|Araport:AT2G21340};
GN ORFNames=F3K23.10 {ECO:0000312|EMBL:AAD23682.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11739388; DOI=10.1074/jbc.m108777200;
RA Li L., He Z., Pandey G.K., Tsuchiya T., Luan S.;
RT "Functional cloning and characterization of a plant efflux carrier for
RT multidrug and heavy metal detoxification.";
RL J. Biol. Chem. 277:5360-5368(2002).
RN [7]
RP GENE FAMILY.
RX PubMed=12603313; DOI=10.1046/j.1432-1033.2003.03418.x;
RA Hvorup R.N., Winnen B., Chang A.B., Jiang Y., Zhou X.F., Saier M.H. Jr.;
RT "The multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter
RT superfamily.";
RL Eur. J. Biochem. 270:799-813(2003).
RN [8]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=26055508; DOI=10.1186/s12870-015-0518-1;
RA Parinthawong N., Cottier S., Buchala A., Nawrath C., Metraux J.P.;
RT "Localization and expression of EDS5H a homologue of the SA transporter
RT EDS5.";
RL BMC Plant Biol. 15:135-135(2015).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:26055508}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:26055508}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8W4G3-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Constitutively expressed in all green tissues.
CC {ECO:0000269|PubMed:26055508}.
CC -!- SIMILARITY: Belongs to the multi antimicrobial extrusion (MATE) (TC
CC 2.A.66.1) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD23682.2; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAM63262.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC006841; AAD23682.2; ALT_FRAME; Genomic_DNA.
DR EMBL; CP002685; AEC07164.1; -; Genomic_DNA.
DR EMBL; AY062577; AAL32655.1; -; mRNA.
DR EMBL; AY128797; AAM91197.1; -; mRNA.
DR EMBL; AK226345; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AY086052; AAM63262.1; ALT_FRAME; mRNA.
DR PIR; B84600; B84600.
DR RefSeq; NP_565509.4; NM_127706.9. [Q8W4G3-1]
DR AlphaFoldDB; Q8W4G3; -.
DR SMR; Q8W4G3; -.
DR STRING; 3702.AT2G21340.1; -.
DR PaxDb; Q8W4G3; -.
DR PRIDE; Q8W4G3; -.
DR ProteomicsDB; 220721; -. [Q8W4G3-1]
DR EnsemblPlants; AT2G21340.1; AT2G21340.1; AT2G21340. [Q8W4G3-1]
DR GeneID; 816673; -.
DR Gramene; AT2G21340.1; AT2G21340.1; AT2G21340. [Q8W4G3-1]
DR KEGG; ath:AT2G21340; -.
DR Araport; AT2G21340; -.
DR TAIR; locus:2050140; AT2G21340.
DR eggNOG; KOG1347; Eukaryota.
DR HOGENOM; CLU_012893_15_2_1; -.
DR InParanoid; Q8W4G3; -.
DR OMA; LTCKSHI; -.
DR OrthoDB; 1486027at2759; -.
DR PhylomeDB; Q8W4G3; -.
DR PRO; PR:Q8W4G3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8W4G3; baseline and differential.
DR Genevisible; Q8W4G3; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; IDA:UniProtKB.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IEA:InterPro.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR CDD; cd13136; MATE_DinF_like; 1.
DR InterPro; IPR044644; DinF-like.
DR InterPro; IPR002528; MATE_fam.
DR PANTHER; PTHR42893; PTHR42893; 1.
DR Pfam; PF01554; MatE; 1.
DR TIGRFAMs; TIGR00797; matE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Membrane; Plastid; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..60
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 61..559
FT /note="Protein DETOXIFICATION 46, chloroplastic"
FT /id="PRO_0000405316"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 489..509
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 519..539
FT /note="Helical"
FT /evidence="ECO:0000305"
FT CONFLICT 35
FT /note="S -> P (in Ref. 5; AAM63262)"
FT /evidence="ECO:0000305"
FT CONFLICT 43
FT /note="A -> S (in Ref. 5; AAM63262)"
FT /evidence="ECO:0000305"
FT CONFLICT 63
FT /note="T -> P (in Ref. 5; AAM63262)"
FT /evidence="ECO:0000305"
FT CONFLICT 70
FT /note="D -> K (in Ref. 5; AAM63262)"
FT /evidence="ECO:0000305"
FT CONFLICT 109
FT /note="T -> N (in Ref. 5; AAM63262)"
FT /evidence="ECO:0000305"
FT CONFLICT 553
FT /note="A -> T (in Ref. 5; AAM63262)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 559 AA; 60000 MW; 8702CA39CAFBCA4C CRC64;
MQIQCKTLTF TVSSIPCNPK LPFPSSLTLR SWNPSFPSFR SSAVSGPKSS LKLNRFLRNC
ASTNQELVVD GETGNGSISE LQGDAANGSI SPVEVEAEVE EVKVDDLATQ SIWGQMKEIV
MFTGPAAGLW LCGPLMSLID TAVIGQGSSL ELAALGPATV ICDYLCYTFM FLSVATSNLV
ATSLARQDKD EVQHQISILL FIGLACGVTM MVLTRLFGSW ALTAFTGVKN ADIVPAANKY
VQIRGLAWPA VLIGWVAQSA SLGMKDSWGP LKALAVASAI NGVGDVVLCT FLGYGIAGAA
WATMVSQVVA AYMMMDALNK KGYSAFSFCV PSPSELLTIF GLAAPVFITM MSKVLFYTLL
VYFATSMGTN IIAAHQVMLQ IYTMSTVWGE PLSQTAQSFM PELLFGINRN LPKARVLLKS
LVIIGATLGI VVGTIGTAVP WLFPGIFTRD KVVTSEMHKV IIPYFLALSI TPSTHSLEGT
LLAGRDLRYI SLSMTGCLAV AGLLLMLLSN GGFGLRGCWY ALVGFQWARF SLSLFRLLSR
DGVLYSEDTS RYAEKVKAA
