DTXS1_METRA
ID DTXS1_METRA Reviewed; 7913 AA.
AC E9FCP4;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 04-MAR-2015, sequence version 2.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Nonribosomal peptide synthetase dtxS1 {ECO:0000305};
DE EC=6.3.2.- {ECO:0000269|PubMed:22232661};
DE AltName: Full=Destruxin synthase dtxS1 {ECO:0000303|PubMed:22232661};
DE Short=DXS {ECO:0000303|PubMed:22367459};
DE AltName: Full=Destruxin synthesis protein 1 {ECO:0000303|PubMed:22232661};
GN Name=dtxS1 {ECO:0000303|PubMed:22232661}; ORFNames=MAA_10043;
OS Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium
OS anisopliae (strain ARSEF 23)).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX NCBI_TaxID=655844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ARSEF 23 / ATCC MYA-3075;
RX PubMed=21253567; DOI=10.1371/journal.pgen.1001264;
RA Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X.,
RA Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S.,
RA Zhong Y., Ma L.-J., St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y.,
RA Wang C.;
RT "Genome sequencing and comparative transcriptomics of the model
RT entomopathogenic fungi Metarhizium anisopliae and M. acridum.";
RL PLoS Genet. 7:E1001264-E1001264(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ARSEF 23 / ATCC MYA-3075;
RX PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA St Leger R.J., Wang C.;
RT "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT adaptation.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
RN [3]
RP FUNCTION, AND INDUCTION.
RX PubMed=22367459; DOI=10.1007/s00294-012-0368-4;
RA Giuliano Garisto Donzelli B., Krasnoff S.B., Moon Y.S., Sun-Moon Y.,
RA Churchill A.C., Gibson D.M.;
RT "Genetic basis of destruxin production in the entomopathogen Metarhizium
RT robertsii.";
RL Curr. Genet. 58:105-116(2012).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND CATALYTIC ACTIVITY.
RX PubMed=22232661; DOI=10.1073/pnas.1115983109;
RA Wang B., Kang Q., Lu Y., Bai L., Wang C.;
RT "Unveiling the biosynthetic puzzle of destruxins in Metarhizium species.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:1287-1292(2012).
CC -!- FUNCTION: Nonribosomal peptide synthetase; part of the gene cluster
CC that mediates the biosynthesis of destruxins, insecticidal cyclic
CC hexadepsipeptides which induce flaccid paralysis and visceral muscle
CC contraction in insects through targeting the calcium channels and
CC vacuolar-type ATPases (PubMed:22367459, PubMed:22232661). The aldo-keto
CC reductase dtxS3 converts alpha-ketoisocaproic acid from deaminated
CC leucine into alpha-hydroxyisocaproic acid (HIC), which is the first
CC substrate for destruxin assembly by dtxS1 (PubMed:22232661). L-
CC aspartate decarboxylase dtxS4 converts aspartic acid into beta-alanine,
CC the last substrate for the destruxin assembly line performed by dtxS1
CC (PubMed:22232661). The nonribosomal peptide synthetase dtxS1
CC synthesizes destruxins B and B2, whereas the cytochrome P450
CC monooxygenase dtxS2 is required to convert destruxin B into other
CC destruxin derivatives, including destructins C, D, A and E
CC (PubMed:22232661). Destruxin E-diol (ED) is further produced in a non-
CC enzymatic manner from destruxin E (PubMed:22232661). Destruxins play an
CC important role in virulence and escape from insect host immune defenses
CC (PubMed:22232661). {ECO:0000269|PubMed:22232661,
CC ECO:0000269|PubMed:22367459}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000269|PubMed:22232661}.
CC -!- INDUCTION: Expression is low during early growth phases and increases
CC with time to reach a steady high level at later growth stages
CC (PubMed:22367459). Also expressed in conidia (PubMed:22367459).
CC {ECO:0000269|PubMed:22367459}.
CC -!- DISRUPTION PHENOTYPE: Impairs the production of destruxins either in CD
CC medium or in silkworm larva (PubMed:22232661). Impairs also the ability
CC to escape insect host immune defenses (PubMed:22232661).
CC {ECO:0000269|PubMed:22232661}.
CC -!- SIMILARITY: Belongs to the NRP synthetase family. {ECO:0000305}.
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DR EMBL; ADNJ02000010; EFY94500.2; -; Genomic_DNA.
DR RefSeq; XP_007826232.2; XM_007828041.2.
DR SMR; E9FCP4; -.
DR PRIDE; E9FCP4; -.
DR EnsemblFungi; EFY94500; EFY94500; MAA_10043.
DR GeneID; 19264329; -.
DR KEGG; maj:MAA_10043; -.
DR HOGENOM; CLU_000022_60_0_1; -.
DR Proteomes; UP000002498; Unassembled WGS sequence.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0008168; F:methyltransferase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR Gene3D; 1.10.1200.10; -; 6.
DR Gene3D; 3.30.300.30; -; 8.
DR Gene3D; 3.30.559.10; -; 7.
DR Gene3D; 3.40.50.12780; -; 3.
DR Gene3D; 3.40.50.150; -; 2.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF00501; AMP-binding; 6.
DR Pfam; PF00668; Condensation; 8.
DR Pfam; PF08241; Methyltransf_11; 1.
DR Pfam; PF00550; PP-binding; 6.
DR SMART; SM00823; PKS_PP; 6.
DR SUPFAM; SSF47336; SSF47336; 6.
DR SUPFAM; SSF53335; SSF53335; 2.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 6.
DR PROSITE; PS00455; AMP_BINDING; 4.
DR PROSITE; PS50075; CARRIER; 6.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 6.
PE 1: Evidence at protein level;
KW Isomerase; Ligase; Phosphopantetheine; Phosphoprotein; Repeat; Virulence.
FT CHAIN 1..7913
FT /note="Nonribosomal peptide synthetase dtxS1"
FT /id="PRO_0000436436"
FT DOMAIN 789..865
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 1865..1941
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 3380..3456
FT /note="Carrier 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 4453..4529
FT /note="Carrier 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 5933..6009
FT /note="Carrier 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 7394..7470
FT /note="Carrier 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 263..662
FT /note="Adenylation 1"
FT /evidence="ECO:0000255"
FT REGION 903..1171
FT /note="Condensation 1"
FT /evidence="ECO:0000255"
FT REGION 1332..1740
FT /note="Adenylation 2"
FT /evidence="ECO:0000255"
FT REGION 1965..2249
FT /note="Condensation 2"
FT /evidence="ECO:0000255"
FT REGION 2863..3255
FT /note="Adenylation 3"
FT /evidence="ECO:0000255"
FT REGION 3496..3761
FT /note="Condensation 3"
FT /evidence="ECO:0000255"
FT REGION 3924..4321
FT /note="Adenylation 4"
FT /evidence="ECO:0000255"
FT REGION 4439..4459
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4545..4837
FT /note="Condensation 4"
FT /evidence="ECO:0000255"
FT REGION 5006..5405
FT /note="Adenylation 5"
FT /evidence="ECO:0000255"
FT REGION 6023..6315
FT /note="Condensation 5"
FT /evidence="ECO:0000255"
FT REGION 6481..6766
FT /note="Adenylation 6"
FT /evidence="ECO:0000255"
FT REGION 7501..7771
FT /note="Condensation 6"
FT /evidence="ECO:0000255"
FT MOD_RES 826
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 1902
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3417
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 4490
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 5970
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 7431
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 7913 AA; 876527 MW; 2F871AB7C5ED426E CRC64;
MPSRILPGKH MPAQGHQRRE ADYWQNALAD CKCATFPAIP ETVEQCLPDA LVEHRFAKLQ
QNGAATSMSN VARAAWALVV ARITNSDDVV FGAVVFGTTR DSNMTIVPVR IETAMDLTIA
AYLGMVQQQE EDMTPFEQTG LSSIMQTCPG AQQACQFQSL LAVHSHESNG ERLDDQRYAL
VLEIRPEEDQ FSALARFDSR VMDHKTVERL LERFELVMTE LCRGGPKMRV QDIHMAGKHD
EEEIWKWNAT VPEAVERCPH HIFEQRSRAH PNKLAVHAWD GVLTYGELDR LSGTLAGRLL
DLGVGPDDLV PLCFEKSMYT VVAMVAVLKA GAGFVLLDPA LPEQRLQIIV DQIQAKIMVS
SVSTYEMSSR MASEVVAISS GFFSEVEPRP CTQSHPPSPS SVMYVVYTSG STGTPKGAAI
THQNHATALY YQAERLGLTD QSRIYDFSSY SFDISIFNAF SALTLGGCLC VPSDYERQDK
LAESITSYNA DFIYLTPTVA RQLSPQKTPT LQTIAFIGEA LHPKDVGIWW DKVRVVNAYG
PSECTTASTL RTSPSTPEEA CSIGKGAGMV TWIVDPNNHN VLLPPGSIGE LLLEGPLIGR
GYLGDAKKNA AAFIKDPAWL IKGASGSKGR SGRLYKTGDL VQYLADGNLK FIGRNDNQVK
IRGNRVELGE VEHALRDCVE TRSVVAEIII PRGSKSSATL AAFLEAEEEE EDDTESEVTA
RMLPVPAEIK DKMAQRLPIY MVPTVLFSMK RLPMTASGKI HRKQLRDIGG RFSARQMVEM
HTKGRAKRQP LSEVEKQVHR IWSQVLGIDA SMIGLDDSFF ELGGDSLGAM EVVSEARKVG
LELTVVDIFK HRSVENVAGQ ARRTTNGSLE ALSPFSLISK DTDVASLIQD MAMDYELDGA
SIQDAFPCTS LQEGLVSLTS KSPGDYVMQG ILELAPDIDV DAFRNAWEQV ATAGQILRTR
ILQSRHHGTL QIVVDESVSW VEATDLDSYL AEDKKKPMEI GQPLVRYALI KDDNAATRWF
VWTIHHSLFD EWSLTLIIDA VTRVYQGKSI TMPHFQPFIK YVEKQRDEKM ANYWRQSLAD
CECPSFPALP PSLEQPVTDG VMEHALHHPQ HDSSVTASTI IRAAWALITS RMTNSDDVVF
GATLSGRGAA VPGVEAIAAP TITTVPVRIR LSREQKVADY LKTVQELATD MIPFEQMGLQ
HIAKLSPGAR QACNFQTLLV IQPHDSSKTQ EVLGKWHLSN QQQSFNTYSL ILEIQLGETM
AARASYDSRV IKPWLVERLL ERLDFLMDQL DNAEPDQAVA EVDTVSGKDI EQLWNWNRTV
PTTSELCIHH VLETQAHSRP DSPALCAWDG QVTYRELNHL AAQLATHLVK RGVGPEVLVP
LYFEKSMWTN VAMLGVLKAG GGFVLLDPSL PEQRLQEMIR QTKAKLLVSS RSNQSASLRL
VPEVVTLGSK FFAELASEPD MNGTKGHKET IGQATPFSTA YVLFTSGSTG TPKGVVITHS
NVTSAVPEHV RCLGYTAASR IYDFASYSFG ASLNNAFAAL MAGGCLCIPS DEDRRSNLAA
SLTALKGTSI LLTPSVAESL SPDSVSGLKT LIFGGEAVRH KDVKPWWGKA KVLTAYGSSE
VTTVATVNTQ ASNMDEAAEI GTGAGGVTWV VDPDDHHKLL PPGCIGELLL EGPLVGSGYL
GDAIKSAEVF IKNPPAWLLE GASGHQGRRG VLYKTGDLVR YNENGNLSYI GRKDAQVKIR
GQRVELGEVE FRVQQCFPEA KQVAAEVVVP CGKKSSPTLA AFLVLDDAQQ ASSSFQTLPV
DTDRMEQLAK QLPSYMVPVL FLAIKEMPMT ASGKMNRRRL RELGGTVTAQ QLADLGTSGQ
VAKRQPRTKL ERQLQALWAR VLNIDASAIG LDDSFYRLGG DSISAMQVSH GARAFHVHIG
VVDILRQRTI SNLAQATGTQ GSTNGATLIN AHAPDQPVTR VDNGPAQLSP IQRLYFVLQN
DPTACFDQFF YLGLRRTTSH QLVSAALETI VRRHGVLRAR FRQNDHGGWE QRISDDVDAS
LLFRVESGVS GTAEAIAKSR AALSITEGPI FSAVLFDKPD HQTLFLTIHH LVIDLVSWRV
LMQELEDIIT NGPLTAPPSM DFPTWSAMQA QYAKESLHAN APDLPESQTN MAYWGMESNP
NLKSGAIVEH FTMDEPTSSL ILGRCNDALG TRPLELMIAA LAYSFSRIFS DCALPAVFSE
GHGREVWHDS LDITTTLGWF STIFPVRVSP GANGLIDFIR ETKDCIRSLS RNGWSYFTSR
FADESNASRF GTDFPVEVMF NYAGFYRNLE RQDGLFEQLS VPENCDPPSC RDVRRFALFD
FDVQVVRGCI VGEVEFHKDM HHRDDIMRWI GEYQSTLRQM ATDLPRISPG WTLSDFPNVF
NTYGDIQEFR NQTLVQLGVE PLDVEDIFPC APLQQGIILA QAKEEANYLR WCDIEIELDA
NQQLDRAKLT DAWKAVVKRH AMLRAVLVDD FPGSSRPMHV VLKDPELGIS WGAETMCFPD
FNKYGLQHRL QVCSFGERRA RLRLHMNHAI TDGFSQSLLC KDLQAAYHDQ LEAAGSYKDF
ILHLENQSQE ARLEFWSQYL AGVEPTLFPT SDCTIPSKVE QVAVPNLDSE SIRAFCAKWD
VTAATIMKLA WGLVLGMYAA TPAPCFGNLY SGRDIPVEGI DSIFGPLIGM IPCCIHLNGS
KGVLDTLKEV QTDYLSTIPY QHSPLAEVHR AAGLLGSRQL FNTLLSYQKN TDETRGNEGG
LTVRVADSYD LTEYDVTIDV VDGTAEIEVL IDFRAGCLST NDAARLAACF SAAVSDIVAN
PLKPTKDLCL LGRRDLDIIW GWNSTVPAAD ERFVHDLIRE RSLAQPHEPA ICAWDGELSY
KELDDLSTRL AGHLHQVGVQ PGALVPLCFE KSLYTSVAML AVVKMGAAFV LLDPSLPEQR
LQSMTQQVGS NLILSSASNR HLCARLCKTV VQVSADFIPT TTSSIPRPKT EPHSTAMFAV
FTSGSTGTPK GIVLTHTNFT TALMHQAEAL GFKETSRVFD FASYAFDLAV HNAFATYVTG
GCLCVPSDED RRGNPASVMK AMRATVVELT PSVSRLIDPA TLPDLETIIM GGEALSVDDV
NRWWGKARIV NIYGPAETHI STVNADAPSP EEATLLGKGS GLVTWIVDPE NRNRLMPPGC
IGELALEGPL VGQGYLNDAQ KTAASFVDDP AWLLQGSPGH SGRHGRVYFS GDLVRYQDHG
SLAFVARKDA QIKIRGQRVE LGEVEHCVQQ CLPEAIRVVV DVITPQGTST PMLSAFLQMD
KAIEVESPAV EVVRIPTDTK NMLSQRLPGY MVPAVFFYMR RLPQTPSGKT DRIQLRQVGG
SFSIQQLATI QTNGQGPKRQ PLSEAERKMQ HIWAQVLNID MASIGLDDNF FELGGDSISV
MKLVAQARKE GLVLTVAAIF RQPTLANVTH QAVAQLEKGP QDLSPFSLLG EGLDVKAFAE
AAMKQHQIPT SVTILDAFPC TPLQEGLLSL SMKRSGNYVL QAKLELSSNI DISRFRNAWE
EVVRSLPILR TRIIEHDSLG LLQAVTDENI SWIETSGLDD YLEADRQQTM GVGQPLARYA
LVGESDARWF VWTVHHVLYD GWSEPLIIKA VNKAYQGSSL DLGPSFQTFI KYIQDSDTSK
MVDYWRQALN DCEAVPFPPP SSSTEVQVAD ASVEQPLPRP RNKKFTASTV IRAAWALVAG
GATDSGDVCF GVTVSGRNAP VPDIERMVGP TFATLPLRVR LSKNQAIADY LETIQQQATD
MIPFEQMGLH RIAKISPGSQ QACNFQTLLV IQPQEEDAGE NVLGEWHDGD QHQWFNTHGL
TILVQISASD ITVKASFNAK VISAWAVQLL LQQLAHVMQQ LDNGGVKALA TIAMATPQDL
EKIWAWNQNV PRPADQCIHE LIADRVRIHP NAPAVCAWDG ELTYQRLDQL SSDLASRLAD
LGVGPAVFVA LCFEKSMWAT VAMLAMVKTG GAFVLLDASL PQQRLKSVVG QVEAAMILTS
STNETLSRQL CENVVIAQDL MTGMQDIVRP LPAPELDSVI YAVFTSGTTG TPKGAIINHR
SSASAVLHQI KGFGYTTETR VYDFSTYSFD GCILNAFTVL AAGGCLCVPT DDGRKNNLAD
SMESLRSNAV FLTPSVAELL SPEQLPSLRS MILGGEAIRV KDIQPWWDAE SVKIITIYGP
SECTPVSMIN PEPTSPENAV RLGWGAGQVT WIVDPEDFNS LTPLGSIGEL LLEGPLVGQG
YLHEPEKTAD AFIMDPVWLL QGVDGRPGRH GRLYKTGDLV RYNQDGSISY IGRKDDQVKL
RGQRVELGEV ERHVRISMPQ AKQAVAEVIV PRSDKSNPAL AVFLQIEDSM MINGVSTENP
PKAAVLLPSA DVLQKLAESL PTYMVPTVFF AMRQLPMGTT GKMDRKELRR IGSSFSAQEL
AQARTAQQGP KRQPASEAER QMQQVWAKVL GIPPASVGRD DNFFQLGGDS ITAMKLVGEA
RKAGLELVVA DMFRYPRLHE AATQAQMLGR NTKVPKMLID GPAEQSFAQA RLWFLEQLYP
GLTWYLMPCI MRLTGPLRLD ALTAAFIALE DRHETLRTTF STREGTNLQH IHAVRSRELT
VVDMSADQES LPHVLSQDET TPFNLENEPG WRVTLYRVGD DEHILSIVLH HIISDGWSID
ILRRELSSFY SAALRGEDPL SNIDPLPIQY KDFSVWQRQQ AQVDEHERQL KYWMTELETS
HPAEFLCDKP RPPTLSGKAD VREVCIDGPV YDKLQEFCRT HGMTPFIVLL AAFRATHYRL
TGDGDGVIGS PNANRDRWEL KDTIGFFVNM QCLRVKIEDE SVTFGELVKL VQSAAISSLA
NQDVPFERLV SKLRKERDLS RHPLIQLVFA VHSQLDLGKF ALEGYEIEYI DQSITTRFDL
EFHFFTEEKG LRGQLIFSTD LFHPDTIDNV LEVFRTVLEK GLNEPQTPVA ALPLMTDGGY
DKLDSMGLIQ VKQTAYPRNS SIVDEFRQQV AACADRVAVK DASSQLTYAQ LDGLSEHVAQ
WLMSKSLAPE TLIGVFCGRS CQSIIAILGI LKANHAYLPF DLKIPASRME GIISSIGHML
VLTGDGVRVP SFALEVEFVM ISEALEHGAR MSSIQRNTCP SPSPSSLAYV MFTSGSTGRP
KGVMVEHRSV LRLIKDDDLR PHGSGIIAHM SNIAFDAATW EIYGALLNGG TVICIDLLTV
LDYAATSRIF AEEKIQAIFI TPALLKQYLS NCPTAIGLLD TIYVGGDRLD PQDVFTARSL
MRGGKVYNGY GPTENTTFST TYRLPVKDIC VNGVAIGRAL SNSGAYVMDT QQCLVPLGVV
GELVVTGDGL ARGYLDSQRN TGRFINVTID GKTVRAYRTG DLARCRPSDG QIECLRRMDA
QVKIRGQRVE LGEIEHVLRN HESVNDAVVT LQQDPQGARL IGFITLDEPD IQHKVQRMEQ
EVDGDDEKKQ VEVWEESFDT DIYAGFDNVK PELIGRDFVG WTSMYDGSEI DKGEMNEWLD
DTIDTILNGG QAGHVLELGS GSGMILFNLC NNGMRSYVGL DPSQKAVEFV TRAAKSMPMA
DKIRMYKGTA TDVGHLGLTT EYDLAIVNSV AQYFPSLKYL TKVVERVLQQ NAKTFFFGDM
RSYAMYKEFT VTRSLYRVGK KPTKDDLRRQ MASMEQMEVE FQVDPAFFTA LPSLLPDLVE
HVEILPKKMQ ATNELSCYRY AAVVHGKGQG LQIRDAEGPW IDFMKDGLHH ESLLKLLQSS
TSPTVSVANI PYSKTIFERH VLDMLGDADG DEDWLSSARQ KANDCASLCA IDLVQLARST
GYQVEISWAR QRSQRGGLDA IFHRHESNGQ RVMFRFPIDD AHQSLSSQPL RQQVKQKIRE
QLRDGMQSQL LPYMIPQAVH ILDKMPVNEN GKVDRRALTE SLQSRATRGP LRQPTSKTQR
QLQAIWAQVL NTDANSIGLD DSFFQVGGDS LGAMRLVGDA RKIGLNLAVA DVFRRPVLRD
MAEGLPLAKA MESIPKTEVD GPVEQSFAQR RLWFLEQLYP GLTWYMMPSA IRLRGHLELD
ALNTAVLALE KRHETLRTTF VSQNDVHLQE VHPFQAKKIR VVSVTEDNLM KALEDDQRTP
FDLKTEPGWR VTVFRLDDTN YLLSIIMHHI VSDGWSVDIL RAELEKFYSA AIRNQDPLAL
VESLPIQYRD FSVWQKQQDQ LDEHQRQLSY WVKQLETSQP AEFLCDKPRP AALSGEAAVE
SLRIDGALYQ QLRTFCRTQS VTPFVALLST FRVAHFFLTG STDATMGTVN ANRDRWEVKD
MIGFFVNMQC IRIRVEAESF KQLVQQVHAT TIASFANQDV PFENIVSQLN RGRDLSRHPL
AQLVFALHSQ MDLGEFVLEG LDTEMVKVPP TTRFDLEFHF FQEQEAFQGE VLYSTDLFDA
QTIRNMLSVF KRVLEAGLGD PNAAITSMSL LSDADYAKLD QMGLVEIDRV DCPDASIVDL
FRQQALLNPD KVAVKDSSSQ LTYAELDQQS DSTARWLAKR CLAPGTLIGV FSSRCCRTVV
ALLGILKANL AYLPFDVHTP RARMEKILGS VDGQTLVLVG NNVQVPEGLD VSFVPIAETL
HEATSEIHIT APNATSLAYV MFTSGSTGNP KGVMINHRGI VRLVKGSNMA SYLPSTPTMA
HITNIAFDVS GWEIYGALLN GGMVVCISAM DVLDFRAVPE IFAREKIQAA IFTPALLKQY
LIQCPPVIGA LTALYVAGDR ADSQDLFMAQ GLMSGHIINA YGPTENSVIS TLYCLQNGER
CVNGVPIGKA ISNSGAYVMD QQQQLVPLGV VGELVVTGDG LARGYTDPGR DIDRFVTVTI
GHKRVKAYRT GDYVRYRTDG QLEFFGRIDG QIKIRGHRVE LGEIEHCLRS HDSVHDAIVV
LQEGQEAQLA AFVTVNEATE DAGQEEDVTD IVNVWGELFD ADTYSTIQDV KPETIGRDFT
GWVSTYTGQD IDKQEMNEWL DDTMATINAY EPRNVLEIGT GTGMILFNLK GVQSYVGLEP
SEKAVEFTVR AAKSMSMLRD KVCVYQGTAA DVKRLPTMLP NLVVINSVAQ YFPSQEYLVK
VIEDVVQLGG VETLFFGDIR SYALNTQFQA SRALRIAGEA ASKDEIRRKM EDIKRADMEL
MVDPAFFTAL AARFKFIHHV EILPKRMKAV NELSCYRYSA VVHLQHDSQL HVHEVESEWI
DFQKNNLNRQ SLLELLGQTS STLAVSNIPF QNTIIERHVV EALDRGQGPD WITSALRNAE
HCPSMSVAGL VELAHLACFQ VEISCARQYS QRGGLDAIFH RQQPSRGDRV LFRFPTDHNR
PSHLLTSRPL RLQLYQTVQE GLFERLQTQL PSYMVPHAIT VVDELPINEN GKVDRRALAA
RTQTRTAARA SIRQPTTDME REMQRIWSHV LHISLDSIGL DDSFFHLGGN SITAMRIVSE
ARKVGFKLSV ADIFRHDVLE DLACHLSPEE EETDIVFVDR PPPVSVLEEI TALGVSVDDV
EDVLPLTSFQ EKIVLDGETV GQHANYFYID LEDLDVSKIQ TSYWATLDKF SILRARFLHL
EGKLWQVVFR QLRLPIHIED VDDVNQAAHQ FCVKDLHEMS STDIPISITL LRHKDGVRLI
LRLSHAQYDG ISFPIILQSL MDEYSGIERP AGPTFGRFLS YAAQQRTKAI TYWTKVLTGS
SLTVPEPILR PKAISGSPQR VYEEAEIDLP QLPSKTTPAT LLSAAWALLL SHITGEDDVV
FGHVVAGRNA AMSGIDEVVG TCLNIVPIRV NLPAAHTPRQ LLLSVQEQFF FGEADLLGFK
DIIEQCTDWP AGTTFDSMIQ HQNIDEHPEI ESAGAASQVQ FFENSHLVPP SLFVVSYPRG
RHLDVKLFGN THILTKEMAK GLIDRLCKIT EELGNLDCSL QVLRDRHGRL SEE
