DTX_COROM
ID DTX_COROM Reviewed; 560 AA.
AC P00587;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Diphtheria toxin;
DE Short=DT;
DE AltName: Full=NAD(+)--diphthamide ADP-ribosyltransferase;
DE EC=2.4.2.36;
DE Contains:
DE RecName: Full=Diphtheria toxin fragment A;
DE Contains:
DE RecName: Full=Diphtheria toxin fragment B;
DE Flags: Precursor;
OS Corynephage omega.
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Lambdavirus; unclassified Lambdavirus.
OX NCBI_TaxID=10714;
OH NCBI_TaxID=1717; Corynebacterium diphtheriae.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6314249; DOI=10.1093/nar/11.19.6589;
RA Ratti G., Rappuoli R., Giannini G.;
RT "The complete nucleotide sequence of the gene coding for diphtheria toxin
RT in the corynephage omega (tox+) genome.";
RL Nucleic Acids Res. 11:6589-6595(1983).
CC -!- FUNCTION: Diphtheria toxin, produced by a phage infecting
CC Corynebacterium diphtheriae, is a proenzyme that, after activation,
CC catalyzes the covalent attachment of the ADP ribose moiety of NAD to
CC elongation factor 2. Fragment A is responsible for enzymatic ADP-
CC ribosylation of elongation factor 2, while fragment B is responsible
CC for binding of toxin to cell receptors and entry of fragment A.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphthamide-[translation elongation factor 2] + NAD(+) = H(+)
CC + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2] +
CC nicotinamide; Xref=Rhea:RHEA:11820, Rhea:RHEA-COMP:10174, Rhea:RHEA-
CC COMP:10175, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:17154,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:82697; EC=2.4.2.36;
CC -!- SUBUNIT: Homodimer.
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DR EMBL; V01536; CAA24778.1; -; Genomic_DNA.
DR PIR; A00728; DOCGPO.
DR BMRB; P00587; -.
DR SMR; P00587; -.
DR DIP; DIP-370N; -.
DR MINT; P00587; -.
DR DrugBank; DB01792; Adenylyl-(3'-5')-uridine 3'-monophosphate.
DR TCDB; 1.C.7.1.1; the diphtheria toxin (dt) family.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0047286; F:NAD+-diphthamide ADP-ribosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.700; -; 1.
DR InterPro; IPR036799; Diphtheria_tox_rcpt-bd_dom_sf.
DR InterPro; IPR036801; Diphtheria_tox_transloc_sf.
DR InterPro; IPR000512; Diphtheria_toxin.
DR InterPro; IPR022406; Diphtheria_toxin_catalytic_dom.
DR InterPro; IPR022404; Diphtheria_toxin_rcpt-bd_dom.
DR InterPro; IPR022405; Diphtheria_toxin_translocation.
DR Pfam; PF02763; Diphtheria_C; 1.
DR Pfam; PF01324; Diphtheria_R; 1.
DR Pfam; PF02764; Diphtheria_T; 1.
DR PIRSF; PIRSF000490; Diphtheria_toxin; 1.
DR PRINTS; PR00769; DPTHRIATOXIN.
DR SUPFAM; SSF49380; SSF49380; 1.
DR SUPFAM; SSF56845; SSF56845; 1.
PE 4: Predicted;
KW Cleavage on pair of basic residues; Disulfide bond; Glycosyltransferase;
KW NAD; Nucleotidyltransferase; Signal; Toxin; Transferase.
FT SIGNAL 1..25
FT CHAIN 26..218
FT /note="Diphtheria toxin fragment A"
FT /id="PRO_0000019347"
FT CHAIN 219..560
FT /note="Diphtheria toxin fragment B"
FT /id="PRO_0000019348"
FT ACT_SITE 173
FT BINDING 46
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 90
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT DISULFID 211..226
FT /evidence="ECO:0000250"
FT DISULFID 486..496
FT /evidence="ECO:0000250"
SQ SEQUENCE 560 AA; 60815 MW; 51F5718361B94EAB CRC64;
MSRKLFASIL IGALLGIGAP PSAHAGADDV VDSSKSFVME NFSSYHGTKP GYVDSIQKGI
QKPKSGTQGN YDDDWKGFYS TDNKYDAAGY SVDNENPLSG KAGGVVKVTY PGLTKVLALK
VDNAETIKKE LGLSLTEPLM EQVGTEEFIK RFGDGASRVV LSLPFAEGSS SVEYINNWEQ
AKALSVELEI NFETRGKRGQ DAMYEYMAQA CAGNRVRRSV GSSLSCINLD WDVIRDKTKT
KIESLKEHGP IKNKMSESPN KTVSEEKAKQ YLEEFHQTAL EHPELSELKT VTGTNPVFAG
ANYAAWAVNV AQVIDSETAD NLEKTTAALS ILPGIGSVMG IADGAVHHNT EEIVAQSIAL
SSLMVAQAIP LVGELVDIGF AAYNFVESII NLFQVVHNSY NRPAYSPGHK TQPFLHDGYA
VSWNTVEDSI IRTGFQGESG HDIKITAENT PLPIAGVLLP TIPGKLDVNK SKTHISVNGR
KIRMRCRAID GDVTFCRPKS PVYVGNGVHA NLHVAFHRSS SEKIHSNEIS SDSIGVLGYQ
KTVDHTKVNS KLSLFFEIKS