ID   ADH2_ZYMMA              Reviewed;         383 AA.
AC   F8DVL8; P06758; Q5NM40;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Alcohol dehydrogenase 2;
DE            EC=1.1.1.1;
DE   AltName: Full=Alcohol dehydrogenase II;
DE            Short=ADH II;
GN   Name=adhB; OrderedLocusNames=Zmob_1541;
OS   Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 /
OS   NCIMB 8938 / NRRL B-806 / ZM1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Zymomonadaceae; Zymomonas.
OX   NCBI_TaxID=555217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10988 / DSM 424 / CCUG 17860 / LMG 404 / NCIMB 8938 / NRRL
RC   B-806 / ZM1;
RX   PubMed=2308827; DOI=10.1093/nar/18.1.187;
RA   Yoon K.-H., Pack M.Y.;
RT   "Nucleotide sequence of the Zymomonas mobilis alcohol dehydrogenase II
RT   gene.";
RL   Nucleic Acids Res. 18:187-187(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10988 / DSM 424 / CCUG 17860 / LMG 404 / NCIMB 8938 / NRRL
RC   B-806 / ZM1;
RX   PubMed=21725006; DOI=10.1128/jb.05395-11;
RA   Pappas K.M., Kouvelis V.N., Saunders E., Brettin T.S., Bruce D., Detter C.,
RA   Balakireva M., Han C.S., Savvakis G., Kyrpides N.C., Typas M.A.;
RT   "Genome sequence of the ethanol-producing Zymomonas mobilis subsp. mobilis
RT   lectotype strain ATCC 10988.";
RL   J. Bacteriol. 193:5051-5052(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC         Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC         Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC         ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: In Z.mobilis there are two isozymes of alcohol
CC       dehydrogenase.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; X17065; CAA34911.1; -; Genomic_DNA.
DR   EMBL; CP002850; AEH63356.1; -; Genomic_DNA.
DR   PIR; A25978; A25978.
DR   RefSeq; WP_014501121.1; NC_017262.1.
DR   AlphaFoldDB; F8DVL8; -.
DR   SMR; F8DVL8; -.
DR   STRING; 555217.Zmob_1541; -.
DR   EnsemblBacteria; AEH63356; AEH63356; Zmob_1541.
DR   KEGG; zmm:Zmob_1541; -.
DR   eggNOG; COG1454; Bacteria.
DR   HOGENOM; CLU_007207_0_0_5; -.
DR   OMA; LPHTAHY; -.
DR   Proteomes; UP000001494; Chromosome.
DR   GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR018211; ADH_Fe_CS.
DR   InterPro; IPR039697; Alcohol_dehydrogenase_Fe.
DR   PANTHER; PTHR11496; PTHR11496; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00913; ADH_IRON_1; 1.
DR   PROSITE; PS00060; ADH_IRON_2; 1.
PE   3: Inferred from homology;
KW   Iron; NAD; Oxidoreductase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..383
FT                   /note="Alcohol dehydrogenase 2"
FT                   /id="PRO_0000414232"
SQ   SEQUENCE   383 AA;  40175 MW;  A182FE4744DE2F84 CRC64;
     MASSTFYIPF VNEMGEGSLE KAIKDLNGSG FKNALIVSDA FMNKSGVVKQ VADLLKTQGI
     NSAVYDGVMP NPTVTAVLEG LKILKDNNSD FVISLGGGSP HDCAKAIALV ATNGGEVKDY
     EGIDKSKKPA LPLMSINTTA GTASEMTRFC IITDEVRHVK MAIVDRHVTP MVSVNDPLLM
     VGMPKGLTAA TGMDALTHAF EAYSSTAATP ITDACALKAA SMIAKNLKTA CDNGKDMPAR
     EAMAYAQFLA GMAFNNASLG YVHAMAHQLG GYYNLPHGVC NAVLLPHVLA YNASVVAGRL
     KDVGVAMGLD IANLGDKEGA EATIQAVRDL AASIGIPANL TELGAKKEDV PLLADHALKD
     ACALTNPRQG DQKEVEELFL SAF