DUO1_ARATH
ID DUO1_ARATH Reviewed; 300 AA.
AC A0A178VEK7; Q9M213;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 07-SEP-2016, sequence version 1.
DT 25-MAY-2022, entry version 35.
DE RecName: Full=Transcription factor DUO1 {ECO:0000303|PubMed:15694308};
DE AltName: Full=Myb-related protein 125 {ECO:0000303|PubMed:19300502};
DE Short=AtMYB125 {ECO:0000303|PubMed:19300502};
DE AltName: Full=Protein DUO POLLEN 1 {ECO:0000303|PubMed:15694308};
GN Name=DUO1 {ECO:0000303|PubMed:15694308};
GN Synonyms=MYB125 {ECO:0000303|PubMed:19300502};
GN OrderedLocusNames=At3g60460 {ECO:0000312|Araport:AT3G60460};
GN ORFNames=T8B10.120 {ECO:0000312|EMBL:CAB81832.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia; TISSUE=Pollen;
RX PubMed=21285328; DOI=10.1105/tpc.110.081059;
RA Borg M., Brownfield L., Khatab H., Sidorova A., Lingaya M., Twell D.;
RT "The R2R3 MYB transcription factor DUO1 activates a male germline-specific
RT regulon essential for sperm cell differentiation in Arabidopsis.";
RL Plant Cell 23:534-549(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-300.
RC STRAIN=cv. Columbia;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=15239831; DOI=10.1186/gb-2004-5-7-r46;
RA Jiang C., Gu X., Peterson T.;
RT "Identification of conserved gene structures and carboxy-terminal motifs in
RT the Myb gene family of Arabidopsis and Oryza sativa L. ssp. indica.";
RL Genome Biol. 5:R46.1-R46.11(2004).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RX PubMed=15694308; DOI=10.1016/j.cub.2005.01.013;
RA Rotman N., Durbarry A., Wardle A., Yang W.C., Chaboud A., Faure J.E.,
RA Berger F., Twell D.;
RT "A novel class of MYB factors controls sperm-cell formation in plants.";
RL Curr. Biol. 15:244-248(2005).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. No-0;
RX PubMed=15618418; DOI=10.1104/pp.104.053165;
RA Durbarry A., Vizir I., Twell D.;
RT "Male germ line development in Arabidopsis. duo pollen mutants reveal
RT gametophytic regulators of generative cell cycle progression.";
RL Plant Physiol. 137:297-307(2005).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia, and cv. No-0;
RX PubMed=19300502; DOI=10.1371/journal.pgen.1000430;
RA Brownfield L., Hafidh S., Borg M., Sidorova A., Mori T., Twell D.;
RT "A plant germline-specific integrator of sperm specification and cell cycle
RT progression.";
RL PLoS Genet. 5:E1000430-E1000430(2009).
RN [9]
RP REGULATION BY MIR159.
RC STRAIN=cv. Columbia;
RX PubMed=21441434; DOI=10.1105/tpc.111.083980;
RA Zheng B., Chen X., McCormick S.;
RT "The anaphase-promoting complex is a dual integrator that regulates both
RT MicroRNA-mediated transcriptional regulation of cyclin B1 and degradation
RT of Cyclin B1 during Arabidopsis male gametophyte development.";
RL Plant Cell 23:1033-1046(2011).
RN [10]
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. No-0;
RX PubMed=22608506; DOI=10.1016/j.cub.2012.04.041;
RA Beale K.M., Leydon A.R., Johnson M.A.;
RT "Gamete fusion is required to block multiple pollen tubes from entering an
RT Arabidopsis ovule.";
RL Curr. Biol. 22:1090-1094(2012).
RN [11]
RP FUNCTION IN DAZ1 AND DAZ2 REGULATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. No-0;
RX PubMed=24876252; DOI=10.1105/tpc.114.124743;
RA Borg M., Rutley N., Kagale S., Hamamura Y., Gherghinoiu M., Kumar S.,
RA Sari U., Esparza-Franco M.A., Sakamoto W., Rozwadowski K., Higashiyama T.,
RA Twell D.;
RT "An EAR-dependent regulatory module promotes male germ cell division and
RT sperm fertility in Arabidopsis.";
RL Plant Cell 26:2098-2113(2014).
RN [12]
RP INDUCTION BY ARID1.
RC STRAIN=cv. Columbia;
RX PubMed=25057814; DOI=10.1371/journal.pgen.1004421;
RA Zheng B., He H., Zheng Y., Wu W., McCormick S.;
RT "An ARID domain-containing protein within nuclear bodies is required for
RT sperm cell formation in Arabidopsis thaliana.";
RL PLoS Genet. 10:E1004421-E1004421(2014).
RN [13]
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=27624837; DOI=10.1104/pp.16.01192;
RA Peters B., Casey J., Aidley J., Zohrab S., Borg M., Twell D.,
RA Brownfield L.;
RT "A conserved cis-regulatory module determines germline fate through
RT activation of the transcription factor DUO1 promoter.";
RL Plant Physiol. 173:280-293(2017).
CC -!- FUNCTION: Transcription activator that acts as a positive regulator of
CC male germline development by promoting both gametic cell specification
CC and cell cycle progression (PubMed:15694308, PubMed:15618418,
CC PubMed:19300502, PubMed:21285328). Binds to canonical MYB sites 5'-
CC AACCGTC-3', 5'-AAACCGC-3' and 5'-AACCGT-3' in promoters to trigger the
CC expression of male germline-specific or enriched genes (e.g. MGH3, GEX2
CC and GCS1), including those required for fertilization (PubMed:21285328,
CC PubMed:19300502). Required for sperm cell specification leading to
CC pollen maturation by activating a germline-specific regulon
CC (PubMed:21285328, PubMed:15694308, PubMed:15618418, PubMed:19300502).
CC Involved in pollen mitosis entry at G2-M transition via the regulation
CC of CYCB1-1, DAZ1 and DAZ2 expression (PubMed:15618418, PubMed:19300502,
CC PubMed:24876252). {ECO:0000269|PubMed:15618418,
CC ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502,
CC ECO:0000269|PubMed:21285328, ECO:0000269|PubMed:24876252}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:15694308}. Note=Accumulates in sperm-cell nuclei.
CC {ECO:0000269|PubMed:15694308}.
CC -!- TISSUE SPECIFICITY: Confined to inflorescences, especially in stamens
CC and pollen. {ECO:0000269|PubMed:15694308}.
CC -!- DEVELOPMENTAL STAGE: Strict spatial and temporal transcriptional
CC activation leading to a specific expression in the male germline cells;
CC this specific expression pattern is dependent of the regulatory region
CC of DUO1 (ROD1) 5'-YAACYGY-3' in its promoter (PubMed:15694308,
CC PubMed:27624837). First observed in the germ cell during or soon after
CC engulfment by the vegetative cell, just after asymmetric division. In
CC maturating pollen, accumulates in germ cells before mitosis and remains
CC high in mature sperm cells. Expression persists during pollen
CC development (PubMed:19300502). {ECO:0000269|PubMed:15694308,
CC ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:27624837}.
CC -!- INDUCTION: Repressed by the microRNA 159 (miR159); the production of
CC miR159 is stimulated by the anaphase promoting complex/cyclosome
CC (APC/C) (PubMed:21441434). Activated by ARID1 in male germline cells
CC via specific histone acetylation regulation (e.g. H3K9Ac)
CC (PubMed:25057814). {ECO:0000269|PubMed:21441434,
CC ECO:0000269|PubMed:25057814}.
CC -!- DISRUPTION PHENOTYPE: Loss of male fertility. Reduced expression of
CC germline-specific genes (e.g. MGH3, GEX2 and GCS1) (PubMed:19300502).
CC Blocked generative cell division resulting in the formation of
CC bicellular pollen grains at anthesis. Pollen fails to enter mitosis at
CC G2-M transition associated with reduced levels of CYCB1-1. Blocked
CC generative cell division followed by endocycle during pollen maturation
CC leads to single larger diploid sperm cell unable to perform
CC fertilization (PubMed:15694308, PubMed:15618418, PubMed:19300502,
CC PubMed:24876252). Enhanced polytubey (e.g. multiple pollen tubes
CC entering ovules) (PubMed:22608506). {ECO:0000269|PubMed:15618418,
CC ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502,
CC ECO:0000269|PubMed:22608506, ECO:0000269|PubMed:24876252}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ADL59373.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB81832.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; HM776521; ADL59373.1; ALT_INIT; mRNA.
DR EMBL; AL138646; CAB81832.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; AEE80064.2; -; Genomic_DNA.
DR EMBL; AF469468; AAL79015.1; -; mRNA.
DR PIR; T47857; T47857.
DR RefSeq; NP_191605.2; NM_115910.2.
DR AlphaFoldDB; A0A178VEK7; -.
DR SMR; A0A178VEK7; -.
DR STRING; 3702.AT3G60460.1; -.
DR ProteomicsDB; 222226; -.
DR EnsemblPlants; AT3G60460.1; AT3G60460.1; AT3G60460.
DR GeneID; 825217; -.
DR Gramene; AT3G60460.1; AT3G60460.1; AT3G60460.
DR KEGG; ath:AT3G60460; -.
DR Araport; AT3G60460; -.
DR TAIR; locus:2103386; AT3G60460.
DR eggNOG; KOG0048; Eukaryota.
DR OMA; FAFWTDD; -.
DR OrthoDB; 1499244at2759; -.
DR PRO; PR:A0A178VEK7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; A0A178VEK7; baseline and differential.
DR GO; GO:0048555; C:generative cell nucleus; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0044839; P:cell cycle G2/M phase transition; IMP:UniProtKB.
DR GO; GO:0055047; P:generative cell mitosis; IMP:UniProtKB.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Activator; Cell cycle; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..300
FT /note="Transcription factor DUO1"
FT /id="PRO_0000440263"
FT DOMAIN 8..64
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 65..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 36..60
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 123..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..138
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 300 AA; 34065 MW; 30388DF4AE8BFCA0 CRC64;
MRKMEAKKEE IKKGPWKAEE DEVLINHVKR YGPRDWSSIR SKGLLQRTGK SCRLRWVNKL
RPNLKNGCKF SADEERTVIE LQSEFGNKWA RIATYLPGRT DNDVKNFWSS RQKRLARILH
NSSDASSSSF NPKSSSSHRL KGKNVKPIRQ SSQGFGLVEE EVTVSSSCSQ MVPYSSDQVG
DEVLRLPDLG VKLEHQPFAF GTDLVLAEYS DSQNDANQQA ISPFSPESRE LLARLDDPFY
YDILGPADSS EPLFALPQPF FEPSPVPRRC RHVSKDEEAD VFLDDFPADM FDQVDPIPSP
