ADH2_ZYMMO
ID ADH2_ZYMMO Reviewed; 383 AA.
AC P0DJA2; P06758; Q5NM40;
DT 14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Alcohol dehydrogenase 2;
DE EC=1.1.1.1;
DE AltName: Full=Alcohol dehydrogenase II;
DE Short=ADH II;
GN Name=adhB; OrderedLocusNames=ZMO1596;
OS Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC Zymomonadaceae; Zymomonas.
OX NCBI_TaxID=264203;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RX PubMed=3584063; DOI=10.1128/jb.169.6.2591-2597.1987;
RA Conway T., Sewell G.W., Osman Y.A., Ingram L.O.;
RT "Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas
RT mobilis.";
RL J. Bacteriol. 169:2591-2597(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RX PubMed=15592456; DOI=10.1038/nbt1045;
RA Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H.,
RA Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J.,
RA Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y., Kang H.L., Lee S.Y., Lee K.J.,
RA Kang H.S.;
RT "The genome sequence of the ethanologenic bacterium Zymomonas mobilis
RT ZM4.";
RL Nat. Biotechnol. 23:63-68(2005).
RN [3]
RP PROTEIN SEQUENCE OF 2-50.
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RX PubMed=2935393; DOI=10.1111/j.1432-1033.1986.tb09366.x;
RA Neale A.D., Scopes R.K., Kelly J.M., Wettenhall R.E.H.;
RT "The two alcohol dehydrogenases of Zymomonas mobilis. Purification by
RT differential dye ligand chromatography, molecular characterisation and
RT physiological roles.";
RL Eur. J. Biochem. 154:119-124(1986).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC -!- SUBUNIT: Homotetramer.
CC -!- MISCELLANEOUS: In Z.mobilis there are two isozymes of alcohol
CC dehydrogenase.
CC -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC family. {ECO:0000305}.
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DR EMBL; M15394; AAA27683.1; -; Genomic_DNA.
DR EMBL; AE008692; AAV90220.1; -; Genomic_DNA.
DR PIR; A25978; A25978.
DR RefSeq; WP_000168720.1; NZ_CP035711.1.
DR PDB; 3OWO; X-ray; 2.07 A; A/B/C/D=1-383.
DR PDB; 3OX4; X-ray; 2.00 A; A/B/C/D=1-383.
DR PDBsum; 3OWO; -.
DR PDBsum; 3OX4; -.
DR AlphaFoldDB; P0DJA2; -.
DR SMR; P0DJA2; -.
DR STRING; 264203.ZMO1596; -.
DR EnsemblBacteria; AAV90220; AAV90220; ZMO1596.
DR GeneID; 58027316; -.
DR KEGG; zmo:ZMO1596; -.
DR eggNOG; COG1454; Bacteria.
DR HOGENOM; CLU_007207_0_0_5; -.
DR OMA; LPHTAHY; -.
DR OrthoDB; 1456634at2; -.
DR BioCyc; MetaCyc:MON-16805; -.
DR BRENDA; 1.1.1.1; 14380.
DR EvolutionaryTrace; P0DJA2; -.
DR Proteomes; UP000001173; Chromosome.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR InterPro; IPR001670; ADH_Fe/GldA.
DR InterPro; IPR018211; ADH_Fe_CS.
DR InterPro; IPR039697; Alcohol_dehydrogenase_Fe.
DR PANTHER; PTHR11496; PTHR11496; 1.
DR Pfam; PF00465; Fe-ADH; 1.
DR PROSITE; PS00913; ADH_IRON_1; 1.
DR PROSITE; PS00060; ADH_IRON_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Iron; NAD; Oxidoreductase;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2935393"
FT CHAIN 2..383
FT /note="Alcohol dehydrogenase 2"
FT /id="PRO_0000087815"
FT CONFLICT 38
FT /note="S -> G (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 45..48
FT /note="SGVV -> GVS (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 4..7
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 10..15
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 18..24
FT /evidence="ECO:0007829|PDB:3OX4"
FT TURN 25..28
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 33..39
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 40..44
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 47..56
FT /evidence="ECO:0007829|PDB:3OX4"
FT TURN 57..59
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 61..68
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 74..86
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 90..97
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 98..112
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 117..120
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 122..124
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 139..141
FT /evidence="ECO:0007829|PDB:3OX4"
FT TURN 144..146
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 148..154
FT /evidence="ECO:0007829|PDB:3OX4"
FT TURN 155..158
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 159..164
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 166..168
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 171..175
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:3OX4"
FT TURN 180..182
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 185..204
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 210..232
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 237..257
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 261..272
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 277..306
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 316..333
FT /evidence="ECO:0007829|PDB:3OX4"
FT STRAND 338..340
FT /evidence="ECO:0007829|PDB:3OX4"
FT TURN 341..344
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 347..349
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 350..357
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 361..365
FT /evidence="ECO:0007829|PDB:3OX4"
FT HELIX 372..381
FT /evidence="ECO:0007829|PDB:3OX4"
SQ SEQUENCE 383 AA; 40145 MW; E1C3A159B1652342 CRC64;
MASSTFYIPF VNEMGEGSLE KAIKDLNGSG FKNALIVSDA FMNKSGVVKQ VADLLKAQGI
NSAVYDGVMP NPTVTAVLEG LKILKDNNSD FVISLGGGSP HDCAKAIALV ATNGGEVKDY
EGIDKSKKPA LPLMSINTTA GTASEMTRFC IITDEVRHVK MAIVDRHVTP MVSVNDPLLM
VGMPKGLTAA TGMDALTHAF EAYSSTAATP ITDACALKAA SMIAKNLKTA CDNGKDMPAR
EAMAYAQFLA GMAFNNASLG YVHAMAHQLG GYYNLPHGVC NAVLLPHVLA YNASVVAGRL
KDVGVAMGLD IANLGDKEGA EATIQAVRDL AASIGIPANL TELGAKKEDV PLLADHALKD
ACALTNPRQG DQKEVEELFL SAF
