DUR3_ARATH
ID DUR3_ARATH Reviewed; 704 AA.
AC F4KD71; Q9FHJ8;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Urea-proton symporter DUR3;
DE Short=AtDUR3;
DE AltName: Full=High-affinity urea active transporter DUR3;
GN Name=DUR3; OrderedLocusNames=At5g45380; ORFNames=MFC19.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=12615950; DOI=10.1105/tpc.007120;
RA Liu L.H., Ludewig U., Frommer W.B., von Wiren N.;
RT "AtDUR3 encodes a new type of high-affinity urea/H+ symporter in
RT Arabidopsis.";
RL Plant Cell 15:790-800(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17869214; DOI=10.1016/j.bbrc.2007.08.177;
RA Hem S., Rofidal V., Sommerer N., Rossignol M.;
RT "Novel subsets of the Arabidopsis plasmalemma phosphoproteome identify
RT phosphorylation sites in secondary active transporters.";
RL Biochem. Biophys. Res. Commun. 363:375-380(2007).
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17672841; DOI=10.1111/j.1365-313x.2007.03223.x;
RA Kojima S., Bohner A., Gassert B., Yuan L., von Wiren N.;
RT "AtDUR3 represents the major transporter for high-affinity urea transport
RT across the plasma membrane of nitrogen-deficient Arabidopsis roots.";
RL Plant J. 52:30-40(2007).
CC -!- FUNCTION: High-affinity urea-proton symporter involved in the active
CC transport of urea across the plasma membrane into root cells. May play
CC an important role in urea uptake by plant cells at low external urea
CC concentrations. {ECO:0000269|PubMed:12615950,
CC ECO:0000269|PubMed:17672841}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=3 uM for urea (at pH 5.0) {ECO:0000269|PubMed:12615950,
CC ECO:0000269|PubMed:17672841};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in root rhizodermis, including root hairs
CC and cortex in more basal root zones. Expressed in shoots.
CC {ECO:0000269|PubMed:12615950, ECO:0000269|PubMed:17672841}.
CC -!- INDUCTION: By nitrogen deficiency in roots.
CC {ECO:0000269|PubMed:12615950, ECO:0000269|PubMed:17672841}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but when grown with urea as a sole source of nitrogen,
CC plants are chlorotic and accumulate increased levels of anthocyanin.
CC {ECO:0000269|PubMed:17672841}.
CC -!- MISCELLANEOUS: The urease inhibitor phenylphosphorodiamidate (PPD) has
CC no effect on urea uptake and translocation or DUR3 gene expression.
CC {ECO:0000305|PubMed:17672841}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09166.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB018113; BAB09166.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95242.1; -; Genomic_DNA.
DR RefSeq; NP_199351.2; NM_123906.5.
DR AlphaFoldDB; F4KD71; -.
DR SMR; F4KD71; -.
DR STRING; 3702.AT5G45380.1; -.
DR TCDB; 2.A.21.6.2; the solute:sodium symporter (sss) family.
DR iPTMnet; F4KD71; -.
DR PaxDb; F4KD71; -.
DR PRIDE; F4KD71; -.
DR ProteomicsDB; 222190; -.
DR EnsemblPlants; AT5G45380.1; AT5G45380.1; AT5G45380.
DR GeneID; 834574; -.
DR Gramene; AT5G45380.1; AT5G45380.1; AT5G45380.
DR KEGG; ath:AT5G45380; -.
DR Araport; AT5G45380; -.
DR TAIR; locus:2163528; AT5G45380.
DR eggNOG; KOG2348; Eukaryota.
DR HOGENOM; CLU_010778_2_1_1; -.
DR InParanoid; F4KD71; -.
DR OMA; MIFITWA; -.
DR OrthoDB; 327628at2759; -.
DR PRO; PR:F4KD71; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KD71; baseline and differential.
DR Genevisible; F4KD71; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015204; F:urea transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IEP:TAIR.
DR GO; GO:0071918; P:urea transmembrane transport; IDA:UniProtKB.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR031155; DUR.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR PANTHER; PTHR46154; PTHR46154; 1.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..704
FT /note="Urea-proton symporter DUR3"
FT /id="PRO_0000418600"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 486..506
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 527..547
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 590..610
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 622..642
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 704 AA; 75913 MW; 2AEBC0335EFD3AF8 CRC64;
MATCPPFDFS TKYYDGDGGC QRQSSFFGGT TVLDQGVGYA VILGFGAFFA VFTSFLVWLE
KRYVGARHTS EWFNTAGRNV KTGLIASVIV SQWTWAATIL QSSNVAWQYG VSGPFWYASG
ATIQVLLFGV MAIEIKRKAP NAHTVCEIVK ARWGTATHIV FLVFCLATNV VVTAMLLLGG
SAVVNALTGV NLYAASFLIP LGVVVYTLAG GLKATFLASY VHSVIVHVAL VVFVFLVYTS
SKELGSPSVV YDRLKDMVAK SRSCTEPLSH HGQACGPVDG NFRGSYLTML SSGGAVFGLI
NIVGNFGTVF VDNGYWVSAI AARPSSTHKG YLLGGLVWFA VPFSLATSLG LGALALDLPI
SKDEADRGLV PPATAIALMG KSGSLLLLTM LFMAVTSAGS SELIAVSSLF TYDIYRTYIN
PRATGRQILK ISRCAVLGFG CFMGILAVVL NKAGVSLGWM YLAMGVLIGS AVIPIAFMLL
WSKANAFGAI LGATSGCVFG IITWLTTAKT QYGRVDLDST GKNGPMLAGN LVAILTGGLI
HAVCSLVRPQ NYDWSTTREI KVVEAYASGD EDVDVPAEEL REEKLRRAKA WIVKWGLVFT
ILIVVIWPVL SLPARVFSRG YFWFWAIVAI AWGTIGSIVI IGLPLVESWD TIKSVCMGMF
TNDRVMKKLD DLNHRLRALT MAVPEAEKIY LLELEKTKKN DEEG
