DUR3_ORYSJ
ID DUR3_ORYSJ Reviewed; 721 AA.
AC Q7XBS0; A0A0N7KSA2; Q9FRN4;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Urea-proton symporter DUR3;
DE Short=OsDUR3;
DE AltName: Full=High-affinity urea active transporter DUR3;
GN Name=DUR3; OrderedLocusNames=Os10g0580400, LOC_Os10g42960;
GN ORFNames=OsJ_32609, OSJNBa0056G17.4;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12615950; DOI=10.1105/tpc.007120;
RA Liu L.H., Ludewig U., Frommer W.B., von Wiren N.;
RT "AtDUR3 encodes a new type of high-affinity urea/H+ symporter in
RT Arabidopsis.";
RL Plant Cell 15:790-800(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22010949; DOI=10.1111/j.1469-8137.2011.03929.x;
RA Wang W.H., Koehler B., Cao F.Q., Liu G.W., Gong Y.Y., Sheng S., Song Q.C.,
RA Cheng X.Y., Garnett T., Okamoto M., Qin R., Mueller-Roeber B., Tester M.,
RA Liu L.H.;
RT "Rice DUR3 mediates high-affinity urea transport and plays an effective
RT role in improvement of urea acquisition and utilization when expressed in
RT Arabidopsis.";
RL New Phytol. 193:432-444(2012).
CC -!- FUNCTION: High-affinity urea-proton symporter involved in the active
CC transport of urea across the plasma membrane into root cells. May play
CC an important role in urea uptake by plant cells at low external urea
CC concentrations. {ECO:0000269|PubMed:22010949}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=10.3 uM for urea (at pH 5.0) {ECO:0000269|PubMed:22010949};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22010949};
CC Multi-pass membrane protein {ECO:0000269|PubMed:22010949}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves and shoots.
CC {ECO:0000269|PubMed:22010949}.
CC -!- INDUCTION: By nitrogen deficiency in roots.
CC {ECO:0000269|PubMed:22010949}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but when grown with urea as the sole source of nitrogen,
CC plants show reduced growth and slight yellowing of shoots.
CC {ECO:0000269|PubMed:22010949}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; AY463691; AAR27948.1; -; mRNA.
DR EMBL; AC018727; AAG46170.1; -; Genomic_DNA.
DR EMBL; DP000086; AAP55189.1; -; Genomic_DNA.
DR EMBL; AP008216; BAF27350.1; -; Genomic_DNA.
DR EMBL; AP014966; BAT12250.1; -; Genomic_DNA.
DR EMBL; CM000147; EAZ17111.1; -; Genomic_DNA.
DR RefSeq; XP_015614910.1; XM_015759424.1.
DR AlphaFoldDB; Q7XBS0; -.
DR SMR; Q7XBS0; -.
DR STRING; 4530.OS10T0580400-01; -.
DR TCDB; 2.A.21.6.3; the solute:sodium symporter (sss) family.
DR PaxDb; Q7XBS0; -.
DR PRIDE; Q7XBS0; -.
DR EnsemblPlants; Os10t0580400-01; Os10t0580400-01; Os10g0580400.
DR GeneID; 4349520; -.
DR Gramene; Os10t0580400-01; Os10t0580400-01; Os10g0580400.
DR KEGG; osa:4349520; -.
DR eggNOG; KOG2348; Eukaryota.
DR HOGENOM; CLU_010778_2_1_1; -.
DR InParanoid; Q7XBS0; -.
DR OMA; MIFITWA; -.
DR OrthoDB; 327628at2759; -.
DR SABIO-RK; Q7XBS0; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000007752; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR Genevisible; Q7XBS0; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015204; F:urea transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0071918; P:urea transmembrane transport; IMP:UniProtKB.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR031155; DUR.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR PANTHER; PTHR46154; PTHR46154; 1.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..721
FT /note="Urea-proton symporter DUR3"
FT /id="PRO_0000418601"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 440..460
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 532..552
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 592..612
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 625..645
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 699..721
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 721 AA; 76416 MW; 3CA04CF150FA800F CRC64;
MASGVCPPAE LGFGAEYYSV VNGVCSRAGS YFGGRPVLTQ AVGYAVVLGF GAFFALFTSF
LVWLEKRYVG SQHTSEWFNT AGRSVKTGLI ASVIVSQWTW AATILQSSNV AWQYGVSGPF
WYASGATIQV LLFGVMAIEI KRKAPNAHTV CEIVRARWGT PAHLVFLTFC LLTNVIVTAM
LLLGGSAVVN ALTGVNVYAA SFLIPLGVVV YTLAGGLKAT FLASYIHSVV VHAVLVVFVF
LVYTSSSKLG SPRVVYDRLM AVASAARDCS ADLSRNGQAC GPVAGNFKGS YLTMLSSGGL
VFGIINIVGN FGTVFVDNGY WMSAIAARPS STHKGYLLGG LVWFAVPFSL ATSLGLGALA
LDLPLTAAEA AKGLVPPATA TALMGKSGSV LLLTMLFMAV TSAGSAELVA VSSLCTYDIY
RTYLNPGASG KQILRVSRAV VLGFGCFMGV LAVVLNVAGV SLGWMYLAMG VIVGSAVIPI
ALLLLWSKAN AVGAMGGAVS GCALGVAVWL TVAKVQYGRV NLDTTGRNAP MLAGNLVSIL
VGGAVHAACS LLRPQHYDWG TSREMITTVE SVHAALDDEL KEERLVHAKR WIVRWGLVFT
AVIVVAWPAL SLPARRYSLG YFTLWAAVAI AWGTVGSVVI ILLPVAESWT TITKVCAGMF
TNDAVYDRLD DVNLRLRAIM GAMPEAEKRY RQLHETEMHP AGTHPANDDD DDNNNNQMMH
S
