DUR3_YEAST
ID DUR3_YEAST Reviewed; 735 AA.
AC P33413; D3DKP5;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Urea active transporter;
GN Name=DUR3; OrderedLocusNames=YHL016C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=TCY1;
RX PubMed=8335627; DOI=10.1128/jb.175.15.4688-4698.1993;
RA Elberry H.M., Majumdar M.L., Cunningham T.S., Sumrada R.A., Cooper T.G.;
RT "Regulation of the urea active transporter gene (DUR3) in Saccharomyces
RT cerevisiae.";
RL J. Bacteriol. 175:4688-4698(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Required for active transport of urea.
CC -!- SUBUNIT: May polymerize.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- INDUCTION: By allophanate or its non-metabolized analog oxalurate.
CC Sensitive to nitrogen catabolite repression.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; L19875; AAA34582.1; -; Genomic_DNA.
DR EMBL; U11582; AAB65069.1; -; Genomic_DNA.
DR EMBL; AY693170; AAT93189.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06669.1; -; Genomic_DNA.
DR PIR; S46830; S46830.
DR RefSeq; NP_011847.1; NM_001179096.1.
DR AlphaFoldDB; P33413; -.
DR SMR; P33413; -.
DR BioGRID; 36407; 45.
DR IntAct; P33413; 17.
DR MINT; P33413; -.
DR STRING; 4932.YHL016C; -.
DR TCDB; 2.A.21.6.1; the solute:sodium symporter (sss) family.
DR iPTMnet; P33413; -.
DR PaxDb; P33413; -.
DR EnsemblFungi; YHL016C_mRNA; YHL016C; YHL016C.
DR GeneID; 856370; -.
DR KEGG; sce:YHL016C; -.
DR SGD; S000001008; DUR3.
DR VEuPathDB; FungiDB:YHL016C; -.
DR eggNOG; KOG2348; Eukaryota.
DR HOGENOM; CLU_010778_2_1_1; -.
DR InParanoid; P33413; -.
DR OMA; MIFITWA; -.
DR BioCyc; YEAST:G3O-31036-MON; -.
DR PRO; PR:P33413; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P33413; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0015489; F:putrescine transmembrane transporter activity; IDA:SGD.
DR GO; GO:0015606; F:spermidine transmembrane transporter activity; IDA:SGD.
DR GO; GO:0015204; F:urea transmembrane transporter activity; IMP:SGD.
DR GO; GO:0015847; P:putrescine transport; IDA:SGD.
DR GO; GO:0015848; P:spermidine transport; IDA:SGD.
DR GO; GO:0015840; P:urea transport; IMP:SGD.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR031155; DUR.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR PANTHER; PTHR46154; PTHR46154; 1.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..735
FT /note="Urea active transporter"
FT /id="PRO_0000105397"
FT TOPO_DOM 1..14
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..85
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..166
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..189
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..253
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..343
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..403
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 425
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 447..454
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 476..496
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 497..517
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 518..618
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 619..639
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 640..650
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 651..671
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 672..735
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 553..572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 58..63
FT /note="GLVAAA -> RLSGCS (in Ref. 1; AAA34582)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 735 AA; 80617 MW; FD1252DB838BF5CF CRC64;
MGEFKPPLPQ GAGYAIVLGL GAVFAGMMVL TTYLLKRYQK EIITAEEFTT AGRSVKTGLV
AAAVVSSWIW CSTLLTSSTK EYADGIFGGY AYAAGACFQI IAFAILAIKT KQMAPNAHTY
LELVRTRYGK IGHGCYLFYA IATNILVTSM LLTSGSAVFS DLTGMNTIAS CFLLPVGVVV
YTLFGGIKAT FLTDYMHTCV IIIIVLVFAF KVYATSDVLG SPGKVYDLVR EAAKRHPVDG
NYQGEYMTMT SKSAGILLII NLIGNFGTVF LDNGYWNKAI SASPAASLKA YAIGGLAWFA
VPSLISLTMG LACLAVETSP NFPTYPDPLT SFQANSGLVL PAAAIAIMGK GGAVASLLMI
FMAVTSAMSA ELIAVSSVFT YDIYREYIDP RASGKKLIYT SHVACIFFGL AMSGFSVGLY
YGGISMGYIY EMMGIIISSA VLPVVLTLCS KDMNLVAAVV SPILGTGLAI MSWLVCTKSL
YKELTVDTTF MDYPMLTGNL VALLSPAIFI PILTYVFKPQ NFDWEKMKDI TRVDETAELV
QADPDIQLYD AEANDKEQEE ETNSLVSDSE KNDVRVNNEK LIEPNLGVVI SNAIFQEDDT
QLQNELDEEQ RELARGLKIA YFLCVFFALA FLVVWPMPMY GSKYIFSKKF FTGWVVVMII
WLFFSAFAVC IYPLWEGRHG IYTTLRGLYW DLSGQTYKLR EWQNSNPQDL HVVTSQISAR
AHRQSSHFGQ VDEII
