ID   ADH3_ENTHI              Reviewed;         395 AA.
AC   Q24857; Q94560;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Alcohol dehydrogenase 3;
DE            Short=ADH;
DE            EC=1.1.1.2;
GN   Name=ADH3;
OS   Entamoeba histolytica.
OC   Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC   Entamoeba.
OX   NCBI_TaxID=5759;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=ATCC 30459 / HM-1:IMSS;
RX   PubMed=8705667; DOI=10.1128/cdli.3.3.270-274.1996;
RA   Kimura A., Hara Y., Kimoto T., Okuno Y., Minekawa Y., Nakabayashi T.;
RT   "Cloning and expression of a putative alcohol dehydrogenase gene of
RT   Entamoeba histolytica and its application to immunological examination.";
RL   Clin. Diagn. Lab. Immunol. 3:270-274(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 30459 / HM-1:IMSS;
RX   PubMed=8611619; DOI=10.1016/0167-4781(96)00014-0;
RA   Rodriguez M.A., Baez-Camargo M., Delgadillo D.M., Orozco E.;
RT   "Cloning and expression of an Entamoeba histolytica NADP+(-)dependent
RT   alcohol dehydrogenase gene.";
RL   Biochim. Biophys. Acta 1306:23-26(1996).
CC   -!- FUNCTION: NADP-dependent ADH activity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a primary alcohol + NADP(+) = an aldehyde + H(+) + NADPH;
CC         Xref=Rhea:RHEA:15937, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.2;
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; D49910; BAA08651.1; -; mRNA.
DR   EMBL; Z48752; CAA88639.1; -; Genomic_DNA.
DR   PIR; S68143; S68143.
DR   AlphaFoldDB; Q24857; -.
DR   SMR; Q24857; -.
DR   STRING; 5759.rna_EHI_198760-1; -.
DR   VEuPathDB; AmoebaDB:EHI5A_080760; -.
DR   VEuPathDB; AmoebaDB:EHI7A_179020; -.
DR   VEuPathDB; AmoebaDB:EHI8A_076400; -.
DR   VEuPathDB; AmoebaDB:EHI_198760; -.
DR   VEuPathDB; AmoebaDB:KM1_213790; -.
DR   eggNOG; KOG3857; Eukaryota.
DR   GO; GO:0008106; F:alcohol dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:1990362; F:butanol dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   CDD; cd08187; BDH; 1.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR018211; ADH_Fe_CS.
DR   InterPro; IPR044731; BDH-like.
DR   PANTHER; PTHR43633; PTHR43633; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00060; ADH_IRON_2; 1.
PE   2: Evidence at transcript level;
KW   NADP; Oxidoreductase.
FT   CHAIN           1..395
FT                   /note="Alcohol dehydrogenase 3"
FT                   /id="PRO_0000087836"
FT   CONFLICT        193
FT                   /note="R -> I (in Ref. 2; CAA88639)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        235..242
FT                   /note="TLENHQDY -> HKKIIKTI (in Ref. 2; CAA88639)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        273..283
FT                   /note="Missing (in Ref. 2; CAA88639)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        307..309
FT                   /note="VMR -> NE (in Ref. 2; CAA88639)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   395 AA;  43485 MW;  506FBBA933631FA9 CRC64;
     MTMLNFTYYN PVRLIYGKGS LDEIEKQHLI PEDARIMMTY GGGSIKKNGV YEEVLKHIKP
     IVEFGGIEPN PSHETCIKAI KIAKENKINF LVAVGGGSII DATKYIALGM EHTYSDDPYD
     ICLKGGKFKV NPAQAKIGVV LTIPATGSET NCWGVISRHA DKLKLPFNNE SVFPTWSIVD
     PCFTMSLPDN QIRNGLVDSF VHCIEQYIGN YHLNPVVEAE TEGVMRTIIG VSHKTLENHQ
     DYQARITFCY AATVALNMSL LCGVTLCGGA HAVGVTLCGG AHAVGHELTS YYGLAHGETL
     AITTPGVMRF NKEKNAKKLI QMGEQVFGIK NSTPEAAIEA TEKWFKSIGM KTRLSEWGKG
     KEEFETIARK FEGNPAGAHK DIDYKGCLQI LNDIY