ID   DUSKY_IPONI             Reviewed;         459 AA.
AC   Q53UH4; Q53UH2; Q53UH3;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Anthocyanidin 3-O-glucoside 2''-O-glucosyltransferase;
DE            EC=2.4.1.297;
DE   AltName: Full=3-O-glucoside-2''-O-glucosyltransferase;
DE            Short=In3GGT;
DE   AltName: Full=Protein dusky;
DE            Short=Dy;
GN   Name=3GGT;
OS   Ipomoea nil (Japanese morning glory) (Pharbitis nil).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=35883;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY,
RP   PATHWAY, AND MUTAGENESIS OF 444-SER--GLY-459.
RC   STRAIN=KK/ZSK-2;
RX   PubMed=15842621; DOI=10.1111/j.1365-313x.2005.02383.x;
RA   Morita Y., Hoshino A., Kikuchi Y., Okuhara H., Ono E., Tanaka Y., Fukui Y.,
RA   Saito N., Nitasaka E., Noguchi H., Iida S.;
RT   "Japanese morning glory dusky mutants displaying reddish-brown or purplish-
RT   gray flowers are deficient in a novel glycosylation enzyme for anthocyanin
RT   biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-
RT   glucosyltransferase, due to 4-bp insertions in the gene.";
RL   Plant J. 42:353-363(2005).
CC   -!- FUNCTION: Glycosyltransferase that mediates the glucosylation of
CC       anthocyanidin 3-O-glucosides to yield anthocyanidin 3-O-sophorosides.
CC       3-O-sophoroside derivatives are required for the bright blue or red
CC       color of flowers. {ECO:0000269|PubMed:15842621}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an anthocyanidin 3-O-beta-D-glucoside + UDP-alpha-D-glucose =
CC         an anthocyanidin 3-O-sophoroside + 2 H(+) + UDP;
CC         Xref=Rhea:RHEA:35419, ChEBI:CHEBI:15378, ChEBI:CHEBI:16307,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:77946;
CC         EC=2.4.1.297; Evidence={ECO:0000269|PubMed:15842621};
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC       {ECO:0000269|PubMed:15842621}.
CC   -!- TISSUE SPECIFICITY: Mainly expressed in the petals and tubes of flower
CC       buds at around 24 hours before flower opening.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AB192314; BAD95881.1; -; mRNA.
DR   EMBL; AB192316; BAD95883.1; -; Genomic_DNA.
DR   EMBL; AB192317; BAD95884.1; -; Genomic_DNA.
DR   EMBL; AB192318; BAD95885.1; -; Genomic_DNA.
DR   RefSeq; XP_019194233.1; XM_019338688.1.
DR   AlphaFoldDB; Q53UH4; -.
DR   SMR; Q53UH4; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   GeneID; 109188143; -.
DR   KEGG; ag:BAD95881; -.
DR   KEGG; ini:109188143; -.
DR   OrthoDB; 508327at2759; -.
DR   UniPathway; UPA00009; -.
DR   GO; GO:0102455; F:anthocyanidin 3-O-glucoside 2''-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0043473; P:pigmentation; IDA:UniProtKB.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF00201; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..459
FT                   /note="Anthocyanidin 3-O-glucoside 2''-O-
FT                   glucosyltransferase"
FT                   /id="PRO_0000422561"
FT   BINDING         276
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         335..337
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         352..360
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         374..377
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         444..459
FT                   /note="SKYMDSFNQKLQDLLG->GFKVHGLFQSETAGSPWMNII: In dy-1;
FT                   reddish-brown or purplish-gray petals phenotype due to
FT                   accumulation of anthocyanidin 3-O-glucoside derivatives."
FT                   /evidence="ECO:0000269|PubMed:15842621"
FT   MUTAGEN         444..459
FT                   /note="SKYMDSFNQKLQDLLG->RFKVHGLFQSETAGSPWMNII: In dy-2;
FT                   reddish-brown or purplish-gray petals phenotype due to
FT                   accumulation of anthocyanidin 3-O-glucoside derivatives."
FT                   /evidence="ECO:0000269|PubMed:15842621"
SQ   SEQUENCE   459 AA;  51170 MW;  8F9BE029B37004EA CRC64;
     MGSQATTYHM AMYPWFGVGH LTGFFRLANK LAGKGHRISF LIPKNTQSKL ESFNLHPHLI
     SFVPIVVPSI PGLPPGAETT SDVPFPSTHL LMEAMDKTQN DIEIILKDLK VDVVFYDFTH
     WLPSLARKIG IKSVFYSTIS PLMHGYALSP ERRVVGKQLT EADMMKAPAS FPDPSIKLHA
     HEARGFTART VMKFGGDITF FDRIFTAVSE SDGLAYSTCR EIEGQFCDYI ETQFQKPVLL
     AGPALPVPSK STMEQKWSDW LGKFKEGSVI YCAFGSECTL RKDKFQELLW GLELTGMPFF
     AALKPPFEAE SIEAAIPEEL KEKIQGRGIV HGEWVQQQLF LQHPSVGCFV SHCGWASLSE
     ALVNDCQIVL LPQVGDQIIN ARIMSVSLKV GVEVEKGEED GVFSRESVCK AVKAVMDEKS
     EIGREVRGNH DKLRGFLLNA DLDSKYMDSF NQKLQDLLG