DUSKY_IPOPU
ID DUSKY_IPOPU Reviewed; 459 AA.
AC Q53UH5;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 36.
DE RecName: Full=Anthocyanidin 3-O-glucoside 2''-O-glucosyltransferase;
DE EC=2.4.1.297;
DE AltName: Full=3-O-glucoside-2''-O-glucosyltransferase;
DE Short=Ip3GGT;
DE AltName: Full=Protein dusky;
DE Short=Dy;
GN Name=3GGT;
OS Ipomoea purpurea (Common morning glory) (Pharbitis purpurea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX NCBI_TaxID=4121;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=YO/FP-39;
RX PubMed=15842621; DOI=10.1111/j.1365-313x.2005.02383.x;
RA Morita Y., Hoshino A., Kikuchi Y., Okuhara H., Ono E., Tanaka Y., Fukui Y.,
RA Saito N., Nitasaka E., Noguchi H., Iida S.;
RT "Japanese morning glory dusky mutants displaying reddish-brown or purplish-
RT gray flowers are deficient in a novel glycosylation enzyme for anthocyanin
RT biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-
RT glucosyltransferase, due to 4-bp insertions in the gene.";
RL Plant J. 42:353-363(2005).
CC -!- FUNCTION: Glycosyltransferase that mediates the glucosylation of
CC anthocyanidin 3-O-glucosides to yield anthocyanidin 3-O-sophorosides.
CC 3-O-sophoroside derivatives are required for the color of flowers.
CC {ECO:0000269|PubMed:15842621}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an anthocyanidin 3-O-beta-D-glucoside + UDP-alpha-D-glucose =
CC an anthocyanidin 3-O-sophoroside + 2 H(+) + UDP;
CC Xref=Rhea:RHEA:35419, ChEBI:CHEBI:15378, ChEBI:CHEBI:16307,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:77946;
CC EC=2.4.1.297;
CC -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB192315; BAD95882.1; -; mRNA.
DR AlphaFoldDB; Q53UH5; -.
DR SMR; Q53UH5; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR KEGG; ag:BAD95882; -.
DR BioCyc; MetaCyc:MON-17863; -.
DR UniPathway; UPA00009; -.
DR GO; GO:0102455; F:anthocyanidin 3-O-glucoside 2''-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR Pfam; PF00201; UDPGT; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Transferase.
FT CHAIN 1..459
FT /note="Anthocyanidin 3-O-glucoside 2''-O-
FT glucosyltransferase"
FT /id="PRO_0000422562"
FT BINDING 276
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 335..337
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 352..360
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 374..377
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 459 AA; 51204 MW; 05A454CE3A09F91A CRC64;
MGSQATTYHM AMYPWFGVGH LTGFFRLANK LAGKGHRISF LIPKNTQSKL ESFNLHPHLI
SFVPIVVPSI PGLPPGAETT SDVPFPSTHL LMEAMDKTQN DIEIILKDLK VDVVFYDFTH
WLPSLARKIG IKSVFYSTIS PLMHGYALSP ERRVVGKQLT EADMMKAPAS FPDPSIKLHA
HEARGFTART VMKFGGDITF FDRIFTAVSE SDGLAYSTCR EIEGQFCDYI ETQFQKPVLL
AGPALPVPSK STMEQKWSDW LGKFKEGSVI YCAFGSECTL RKDKFQELLW GLELTGMPFF
AALKPPFETE SVEAAIPEEL KEKIQGRGIV HGEWVQQQLF LQHPSVGCFV SHCGWASLSE
ALVNDCQIVL LPQVGDQIIN ARIMSVSLKV GVEVEKGEED GVFSRESVCK AVKAVMDEKS
EIGREVRGNH DKLRGFLMNA DLDSKYMDSF NQKLQDLLG
