ADH7_MOUSE
ID ADH7_MOUSE Reviewed; 374 AA.
AC Q64437;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=All-trans-retinol dehydrogenase [NAD(+)] ADH7 {ECO:0000305};
DE EC=1.1.1.105 {ECO:0000250|UniProtKB:P40394};
DE AltName: Full=ADH-C2;
DE AltName: Full=Alcohol dehydrogenase 7;
DE AltName: Full=Alcohol dehydrogenase class 4 mu/sigma chain;
DE EC=1.1.1.1 {ECO:0000250|UniProtKB:P40394};
DE AltName: Full=Alcohol dehydrogenase class IV mu/sigma chain;
DE AltName: Full=Gastric alcohol dehydrogenase;
DE AltName: Full=Omega-hydroxydecanoate dehydrogenase ADH7 {ECO:0000250|UniProtKB:P40394};
DE EC=1.1.1.66 {ECO:0000250|UniProtKB:P40394};
DE AltName: Full=Retinol dehydrogenase;
GN Name=Adh7; Synonyms=Adh-3, Adh3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=FVB/N; TISSUE=Gastric mucosa;
RX PubMed=7738026; DOI=10.1074/jbc.270.18.10868;
RA Zgombic-Knight M., Ang H.L., Foglio M.H., Duester G.;
RT "Cloning of the mouse class IV alcohol dehydrogenase (retinol
RT dehydrogenase) cDNA and tissue-specific expression patterns of the murine
RT ADH gene family.";
RL J. Biol. Chem. 270:10868-10877(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RX PubMed=9126489; DOI=10.1006/geno.1997.4637;
RA Zgombic-Knight M., Deltour L., Haselbeck R.J., Foglio M.H., Duester G.;
RT "Gene structure and promoter for Adh-3 encoding mouse class IV alcohol
RT dehydrogenase (retinol dehydrogenase).";
RL Genomics 41:105-109(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Catalyzes the NAD-dependent oxidation of all-trans-retinol,
CC alcohol, aldehyde and omega-hydroxy fatty acids and their derivatives.
CC Oxidizes preferentially all trans-retinol, all-trans-4-hydroxyretinol,
CC 9-cis-retinol, 2-hexenol, and long chain omega-hydroxy fatty acids such
CC as juniperic acid. In vitro can also catalyzes the NADH-dependent
CC reduction of all-trans-retinal and aldehydes and their derivatives.
CC Reduces preferentially all trans-retinal, all-trans-4-oxoretinal and
CC hexanal. Catalyzes in the oxidative direction with higher efficiency.
CC Therefore may participate in retinoid metabolism, fatty acid omega-
CC oxidation, and elimination of cytotoxic aldehydes produced by lipid
CC peroxidation. {ECO:0000250|UniProtKB:P40394}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10737;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:10738;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=10-hydroxydecanoate + NAD(+) = 10-oxodecanoate + H(+) + NADH;
CC Xref=Rhea:RHEA:20880, ChEBI:CHEBI:11305, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58022; EC=1.1.1.66;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20881;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-retinol + NAD(+) = all-trans-retinal + H(+) + NADH;
CC Xref=Rhea:RHEA:21284, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336,
CC ChEBI:CHEBI:17898, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.1.1.105; Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21285;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-retinol + NAD(+) = 9-cis-retinal + H(+) + NADH;
CC Xref=Rhea:RHEA:42052, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:78272, ChEBI:CHEBI:78273;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42053;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-3,4-didehydroretinol + NAD(+) = all-trans-3,4-
CC didehydroretinal + H(+) + NADH; Xref=Rhea:RHEA:55940,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:28537, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:132246;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55941;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-4-hydroxyretinol + NAD(+) = all-trans-4-
CC hydroxyretinal + H(+) + NADH; Xref=Rhea:RHEA:55936,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:132259, ChEBI:CHEBI:139346;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55937;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-4-oxoretinol + NAD(+) = all-trans-4-oxoretinal +
CC H(+) + NADH; Xref=Rhea:RHEA:60632, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:44597, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:139347; Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:60634;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=12-hydroxydodecanoate + NAD(+) = 12-oxododecanoate + H(+) +
CC NADH; Xref=Rhea:RHEA:60980, ChEBI:CHEBI:15378, ChEBI:CHEBI:36204,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:144067;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60981;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16-hydroxyhexadecanoate + NAD(+) = 16-oxohexadecanoate + H(+)
CC + NADH; Xref=Rhea:RHEA:60984, ChEBI:CHEBI:15378, ChEBI:CHEBI:55329,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:144068;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60985;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexan-1-ol + NAD(+) = H(+) + hexanal + NADH;
CC Xref=Rhea:RHEA:60972, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:87393, ChEBI:CHEBI:88528;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60973;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:60974;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-hex-2-en-1-ol + NAD(+) = (E)-hex-2-enal + H(+) + NADH;
CC Xref=Rhea:RHEA:60988, ChEBI:CHEBI:15378, ChEBI:CHEBI:28913,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:141205;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60989;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:60990;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-4-hydroxynon-2-en-1-ol + NAD(+) = (E)-4-hydroxynon-2-enal
CC + H(+) + NADH; Xref=Rhea:RHEA:60976, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58968,
CC ChEBI:CHEBI:142617; Evidence={ECO:0000250|UniProtKB:P40394};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:60978;
CC Evidence={ECO:0000250|UniProtKB:P40394};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- ACTIVITY REGULATION: Retinol oxidation is inhibited by the detergent
CC Tween 80. Ethanol inhibits both all-trans-retinol and 9-cis-retinol
CC oxidation. 13-cis-retinol is an effective competitive inhibitor of the
CC 9-cis-retinol oxidation. All-trans-retinoic acid is a powerful
CC inhibitor of all-trans-retinol oxidation. 13-cis-retinoic acid is a
CC powerful inhibitor of all-trans-retinol oxidation. Cimetidine
CC competitively inhibited ethanol oxidation.
CC {ECO:0000250|UniProtKB:P40394}.
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- TISSUE SPECIFICITY: High expression in the stomach mucosa. Lower
CC expression in eye, thymus, skin and ovary. Very low expression in small
CC intestine, liver and uterus.
CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC family. Class-IV subfamily. {ECO:0000305}.
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DR EMBL; U20257; AAA76735.1; -; mRNA.
DR EMBL; U76734; AAC53124.1; -; Genomic_DNA.
DR EMBL; U76728; AAC53124.1; JOINED; Genomic_DNA.
DR EMBL; U76729; AAC53124.1; JOINED; Genomic_DNA.
DR EMBL; U76730; AAC53124.1; JOINED; Genomic_DNA.
DR EMBL; U76727; AAC53124.1; JOINED; Genomic_DNA.
DR EMBL; U76731; AAC53124.1; JOINED; Genomic_DNA.
DR EMBL; U76733; AAC53124.1; JOINED; Genomic_DNA.
DR EMBL; U76732; AAC53124.1; JOINED; Genomic_DNA.
DR EMBL; AC079682; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS38651.1; -.
DR PIR; A56436; A56436.
DR RefSeq; NP_033756.2; NM_009626.4.
DR RefSeq; XP_006500982.1; XM_006500919.3.
DR AlphaFoldDB; Q64437; -.
DR SMR; Q64437; -.
DR STRING; 10090.ENSMUSP00000087633; -.
DR iPTMnet; Q64437; -.
DR PhosphoSitePlus; Q64437; -.
DR jPOST; Q64437; -.
DR PaxDb; Q64437; -.
DR PeptideAtlas; Q64437; -.
DR PRIDE; Q64437; -.
DR ProteomicsDB; 296183; -.
DR Antibodypedia; 25891; 369 antibodies from 31 providers.
DR DNASU; 11529; -.
DR Ensembl; ENSMUST00000090171; ENSMUSP00000087633; ENSMUSG00000055301.
DR GeneID; 11529; -.
DR KEGG; mmu:11529; -.
DR UCSC; uc008rnd.2; mouse.
DR CTD; 131; -.
DR MGI; MGI:87926; Adh7.
DR VEuPathDB; HostDB:ENSMUSG00000055301; -.
DR eggNOG; KOG0022; Eukaryota.
DR GeneTree; ENSGT00940000162708; -.
DR HOGENOM; CLU_026673_14_0_1; -.
DR InParanoid; Q64437; -.
DR OMA; MVISFHT; -.
DR OrthoDB; 664798at2759; -.
DR PhylomeDB; Q64437; -.
DR TreeFam; TF300429; -.
DR Reactome; R-MMU-71384; Ethanol oxidation.
DR BioGRID-ORCS; 11529; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Adh7; mouse.
DR PRO; PR:Q64437; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q64437; protein.
DR Bgee; ENSMUSG00000055301; Expressed in esophagus and 101 other tissues.
DR ExpressionAtlas; Q64437; baseline and differential.
DR Genevisible; Q64437; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IDA:MGI.
DR GO; GO:0004024; F:alcohol dehydrogenase activity, zinc-dependent; ISO:MGI.
DR GO; GO:0004031; F:aldehyde oxidase activity; ISO:MGI.
DR GO; GO:0035276; F:ethanol binding; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0051287; F:NAD binding; ISO:MGI.
DR GO; GO:0004745; F:NAD-retinol dehydrogenase activity; IDA:MGI.
DR GO; GO:0050153; F:omega-hydroxydecanoate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016491; F:oxidoreductase activity; TAS:MGI.
DR GO; GO:0048019; F:receptor antagonist activity; ISO:MGI.
DR GO; GO:0019841; F:retinol binding; ISO:MGI.
DR GO; GO:0008270; F:zinc ion binding; ISO:MGI.
DR GO; GO:0006068; P:ethanol catabolic process; IMP:MGI.
DR GO; GO:0006067; P:ethanol metabolic process; ISO:MGI.
DR GO; GO:0006069; P:ethanol oxidation; ISO:MGI.
DR GO; GO:0010430; P:fatty acid omega-oxidation; ISO:MGI.
DR GO; GO:0009617; P:response to bacterium; ISO:MGI.
DR GO; GO:0045471; P:response to ethanol; ISO:MGI.
DR GO; GO:0042573; P:retinoic acid metabolic process; IMP:MGI.
DR GO; GO:0001523; P:retinoid metabolic process; ISS:UniProtKB.
DR GO; GO:0042572; P:retinol metabolic process; IMP:MGI.
DR InterPro; IPR013149; ADH-like_C.
DR InterPro; IPR013154; ADH_N.
DR InterPro; IPR002328; ADH_Zn_CS.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR028635; Zinc_ADH_IV.
DR PANTHER; PTHR43880:SF2; PTHR43880:SF2; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF00107; ADH_zinc_N; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SUPFAM; SSF50129; SSF50129; 2.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00059; ADH_ZINC; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Lipid metabolism; Metal-binding; NAD; Oxidoreductase;
KW Reference proteome; Zinc.
FT CHAIN 1..374
FT /note="All-trans-retinol dehydrogenase [NAD(+)] ADH7"
FT /id="PRO_0000160692"
FT BINDING 47
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 68
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 98
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 101
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 104
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 112
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 174
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 199..204
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 223
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 228
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 292..294
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 369
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT CONFLICT 120
FT /note="R -> C (in Ref. 1; AAA76735 and 2; AAC53124)"
FT /evidence="ECO:0000305"
FT CONFLICT 262
FT /note="I -> V (in Ref. 1; AAA76735 and 2; AAC53124)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 374 AA; 39904 MW; D615119A23B41843 CRC64;
MGTAGKVIKC KAAVLWGVNQ PFSIEEIEVA PPKAKEVRVK ILATGICRTD DHIIKGSMVS
KFPVIVGHEA VGVVESVGEG VTTVRPGDKV IPLFLPQCRE CNACLNPEGN LCIRSDLTGR
GVLADGTTRF TCKGKPVQHF MNTSTFTEYT VLDESSVAKV DGAAPPEKAC LIGCGFSTGY
GAAVKTAKVT PGSTCVVFGL GGVGLSVIMG CKAAGASRII GIDINKDKFQ KALAVGATEC
ISPKDSTKPI SEVLSDMTGN TIQYTFEVIG RLETMVDALS SCHMNYGTSV VVGAPPSAKM
LTYDPMLLFT GRTWKGCVFG GWKSRDDVPK LVTEFLEKKF DLDQLITHTL PFNNINEGFE
LLYSGKSIRT VLTF
