ID   ADHA_CLOAB              Reviewed;         389 AA.
AC   Q04944;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2001, sequence version 2.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=NADH-dependent butanol dehydrogenase A;
DE            EC=1.1.1.-;
DE   AltName: Full=BDH I;
GN   Name=bdhA; OrderedLocusNames=CA_C3299;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=1385386; DOI=10.1128/jb.174.22.7149-7158.1992;
RA   Walter K.A., Bennett G.N., Papoutsakis E.T.;
RT   "Molecular characterization of two Clostridium acetobutylicum ATCC 824
RT   butanol dehydrogenase isozyme genes.";
RL   J. Bacteriol. 174:7149-7158(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
RN   [3]
RP   PROTEIN SEQUENCE OF N-TERMINUS.
RX   PubMed=1809209; DOI=10.1111/j.1749-6632.1991.tb18567.x;
RA   Petersen D.J., Welch R.W., Walter K.A., Mermelstein L.D., Papoutsakis E.T.,
RA   Rudolph F.B., Bennett G.N.;
RT   "Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium
RT   acetobutylicum.";
RL   Ann. N. Y. Acad. Sci. 646:94-98(1991).
CC   -!- PATHWAY: Alcohol metabolism; butanol biosynthesis.
CC   -!- SUBUNIT: Homodimer.
CC   -!- MISCELLANEOUS: Primarily NADH-dependent, although some NADPH-dependent
CC       activity was observed.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; M96945; AAA23206.1; -; Genomic_DNA.
DR   EMBL; AE001437; AAK81232.1; -; Genomic_DNA.
DR   PIR; A47013; A47013.
DR   PIR; E97305; E97305.
DR   RefSeq; NP_349892.1; NC_003030.1.
DR   RefSeq; WP_010966572.1; NC_003030.1.
DR   AlphaFoldDB; Q04944; -.
DR   SMR; Q04944; -.
DR   STRING; 272562.CA_C3299; -.
DR   EnsemblBacteria; AAK81232; AAK81232; CA_C3299.
DR   GeneID; 44999793; -.
DR   KEGG; cac:CA_C3299; -.
DR   PATRIC; fig|272562.8.peg.3477; -.
DR   eggNOG; COG1979; Bacteria.
DR   HOGENOM; CLU_007207_0_4_9; -.
DR   OMA; CTMALNG; -.
DR   OrthoDB; 717704at2; -.
DR   BioCyc; MetaCyc:BDHACLOS-MON; -.
DR   UniPathway; UPA00743; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IDA:CACAO.
DR   GO; GO:1990362; F:butanol dehydrogenase activity; IDA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0071271; P:1-butanol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd08187; BDH; 1.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR018211; ADH_Fe_CS.
DR   InterPro; IPR044731; BDH-like.
DR   PANTHER; PTHR43633; PTHR43633; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00913; ADH_IRON_1; 1.
DR   PROSITE; PS00060; ADH_IRON_2; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..389
FT                   /note="NADH-dependent butanol dehydrogenase A"
FT                   /id="PRO_0000087822"
FT   CONFLICT        217
FT                   /note="A -> R (in Ref. 1; AAA23206)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   389 AA;  42982 MW;  49E3B120F0E22252 CRC64;
     MLSFDYSIPT KVFFGKGKID VIGEEIKKYG SRVLIVYGGG SIKRNGIYDR ATAILKENNI
     AFYELSGVEP NPRITTVKKG IEICRENNVD LVLAIGGGSA IDCSKVIAAG VYYDGDTWDM
     VKDPSKITKV LPIASILTLS ATGSEMDQIA VISNMETNEK LGVGHDDMRP KFSVLDPTYT
     FTVPKNQTAA GTADIMSHTF ESYFSGVEGA YVQDGIAEAI LRTCIKYGKI AMEKTDDYEA
     RANLMWASSL AINGLLSLGK DRKWSCHPME HELSAYYDIT HGVGLAILTP NWMEYILNDD
     TLHKFVSYGI NVWGIDKNKD NYEIAREAIK NTREYFNSLG IPSKLREVGI GKDKLELMAK
     QAVRNSGGTI GSLRPINAED VLEIFKKSY