ADHA_RHIME
ID ADHA_RHIME Reviewed; 340 AA.
AC O31186;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Alcohol dehydrogenase;
DE EC=1.1.1.1;
GN Name=adhA; OrderedLocusNames=RA0704; ORFNames=SMa1296;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymA (megaplasmid 1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=9659380; DOI=10.1016/s0167-4838(98)00048-x;
RA Willis L.B., Walker G.C.;
RT "Identification of the Rhizobium meliloti alcohol dehydrogenase gene (adhA)
RT and heterologous expression in Alcaligenes eutrophus.";
RL Biochim. Biophys. Acta 1384:197-203(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481432; DOI=10.1073/pnas.161294798;
RA Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F.,
RA Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L.,
RA Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C.,
RA Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A.,
RA Long S.R.;
RT "Nucleotide sequence and predicted functions of the entire Sinorhizobium
RT meliloti pSymA megaplasmid.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC family. {ECO:0000305}.
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DR EMBL; AF031940; AAB87463.1; -; Genomic_DNA.
DR EMBL; AE006469; AAK65362.1; -; Genomic_DNA.
DR PIR; H95349; H95349.
DR RefSeq; NP_435950.1; NC_003037.1.
DR RefSeq; WP_010967682.1; NC_003037.1.
DR AlphaFoldDB; O31186; -.
DR SMR; O31186; -.
DR EnsemblBacteria; AAK65362; AAK65362; SMa1296.
DR GeneID; 25012428; -.
DR GeneID; 61599472; -.
DR KEGG; sme:SMa1296; -.
DR PATRIC; fig|266834.11.peg.726; -.
DR HOGENOM; CLU_026673_20_1_5; -.
DR OMA; QYAHNVF; -.
DR PRO; PR:O31186; -.
DR Proteomes; UP000001976; Plasmid pSymA.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR InterPro; IPR013149; ADH-like_C.
DR InterPro; IPR013154; ADH_N.
DR InterPro; IPR002328; ADH_Zn_CS.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020843; PKS_ER.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF00107; ADH_zinc_N; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SUPFAM; SSF50129; SSF50129; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00059; ADH_ZINC; 1.
PE 3: Inferred from homology;
KW Metal-binding; NAD; Oxidoreductase; Plasmid; Reference proteome; Zinc.
FT CHAIN 1..340
FT /note="Alcohol dehydrogenase"
FT /id="PRO_0000160748"
FT BINDING 40
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 63
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
SQ SEQUENCE 340 AA; 36235 MW; C1A04B5FEE5C95E1 CRC64;
MTMTAAVVRE FGKPLVIEEV PVPQPGPGQV LIKYEATGVC HTDLHAAKGD WPVRPNPPFI
PGHEGVGYVA KLGAEVTRLK EGDRVGVPWL HTACGCCTPC RTGWETLCGS QQNTGYSVDG
TFAQYGLADP DFVGRLPARL EFGPAAPVLC AGVTVYKGLK ETEVRPGEWV LVSGIGGLGH
MAVQYAKAMG MHVAAADIFP DKLALAEKLG ADLVVDARAP DAVEEVQRRT GGLHGALVTA
VSPKAMEQAY SMLRSKGTMA LVGLPPGQIC LPVFDTVLKR ITVRGSIVGT RQDLEEALEF
AGEGKVAAHF SWDKIENINA IFERMEEGKI DGRIVLDLNG
