ID   ADHB_CLOAB              Reviewed;         390 AA.
AC   Q04945;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=NADH-dependent butanol dehydrogenase B;
DE            EC=1.1.1.-;
DE   AltName: Full=BDH II;
GN   Name=bdhB; OrderedLocusNames=CA_C3298;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=1385386; DOI=10.1128/jb.174.22.7149-7158.1992;
RA   Walter K.A., Bennett G.N., Papoutsakis E.T.;
RT   "Molecular characterization of two Clostridium acetobutylicum ATCC 824
RT   butanol dehydrogenase isozyme genes.";
RL   J. Bacteriol. 174:7149-7158(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
RN   [3]
RP   PROTEIN SEQUENCE OF N-TERMINUS.
RX   PubMed=1809209; DOI=10.1111/j.1749-6632.1991.tb18567.x;
RA   Petersen D.J., Welch R.W., Walter K.A., Mermelstein L.D., Papoutsakis E.T.,
RA   Rudolph F.B., Bennett G.N.;
RT   "Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium
RT   acetobutylicum.";
RL   Ann. N. Y. Acad. Sci. 646:94-98(1991).
CC   -!- PATHWAY: Alcohol metabolism; butanol biosynthesis.
CC   -!- SUBUNIT: Homodimer.
CC   -!- MISCELLANEOUS: Primarily NADH-dependent, although some NADPH-dependent
CC       activity was observed.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; M96946; AAA23207.1; -; Genomic_DNA.
DR   EMBL; AE001437; AAK81231.1; -; Genomic_DNA.
DR   PIR; B47013; B47013.
DR   PIR; D97305; D97305.
DR   RefSeq; NP_349891.1; NC_003030.1.
DR   RefSeq; WP_010966571.1; NC_003030.1.
DR   AlphaFoldDB; Q04945; -.
DR   SMR; Q04945; -.
DR   STRING; 272562.CA_C3298; -.
DR   EnsemblBacteria; AAK81231; AAK81231; CA_C3298.
DR   GeneID; 44999792; -.
DR   KEGG; cac:CA_C3298; -.
DR   PATRIC; fig|272562.8.peg.3476; -.
DR   eggNOG; COG1979; Bacteria.
DR   HOGENOM; CLU_007207_0_4_9; -.
DR   OMA; GTLQMGY; -.
DR   OrthoDB; 717704at2; -.
DR   BioCyc; MetaCyc:BDHBCLOS-MON; -.
DR   UniPathway; UPA00743; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:1990362; F:butanol dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0071271; P:1-butanol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd08187; BDH; 1.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR018211; ADH_Fe_CS.
DR   InterPro; IPR044731; BDH-like.
DR   PANTHER; PTHR43633; PTHR43633; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00913; ADH_IRON_1; 1.
DR   PROSITE; PS00060; ADH_IRON_2; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..390
FT                   /note="NADH-dependent butanol dehydrogenase B"
FT                   /id="PRO_0000087823"
SQ   SEQUENCE   390 AA;  43287 MW;  BDAA32635C17CAB9 CRC64;
     MVDFEYSIPT RIFFGKDKIN VLGRELKKYG SKVLIVYGGG SIKRNGIYDK AVSILEKNSI
     KFYELAGVEP NPRVTTVEKG VKICRENGVE VVLAIGGGSA IDCAKVIAAA CEYDGNPWDI
     VLDGSKIKRV LPIASILTIA ATGSEMDTWA VINNMDTNEK LIAAHPDMAP KFSILDPTYT
     YTVPTNQTAA GTADIMSHIF EVYFSNTKTA YLQDRMAEAL LRTCIKYGGI ALEKPDDYEA
     RANLMWASSL AINGLLTYGK DTNWSVHLME HELSAYYDIT HGVGLAILTP NWMEYILNND
     TVYKFVEYGV NVWGIDKEKN HYDIAHQAIQ KTRDYFVNVL GLPSRLRDVG IEEEKLDIMA
     KESVKLTGGT IGNLRPVNAS EVLQIFKKSV