DUT_METBU
ID DUT_METBU Reviewed; 170 AA.
AC Q12XH1;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Probable deoxyuridine 5'-triphosphate nucleotidohydrolase {ECO:0000255|HAMAP-Rule:MF_00635};
DE Short=dUTPase {ECO:0000255|HAMAP-Rule:MF_00635};
DE EC=3.6.1.23 {ECO:0000255|HAMAP-Rule:MF_00635};
DE AltName: Full=dUTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_00635};
GN Name=dut {ECO:0000255|HAMAP-Rule:MF_00635}; OrderedLocusNames=Mbur_0910;
OS Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 /
OS ACE-M).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanococcoides.
OX NCBI_TaxID=259564;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6242 / NBRC 107633 / OCM 468 / ACE-M;
RX PubMed=19404327; DOI=10.1038/ismej.2009.45;
RA Allen M.A., Lauro F.M., Williams T.J., Burg D., Siddiqui K.S.,
RA De Francisci D., Chong K.W., Pilak O., Chew H.H., De Maere M.Z., Ting L.,
RA Katrib M., Ng C., Sowers K.R., Galperin M.Y., Anderson I.J., Ivanova N.,
RA Dalin E., Martinez M., Lapidus A., Hauser L., Land M., Thomas T.,
RA Cavicchioli R.;
RT "The genome sequence of the psychrophilic archaeon, Methanococcoides
RT burtonii: the role of genome evolution in cold adaptation.";
RL ISME J. 3:1012-1035(2009).
CC -!- FUNCTION: This enzyme is involved in nucleotide metabolism: it produces
CC dUMP, the immediate precursor of thymidine nucleotides and it decreases
CC the intracellular concentration of dUTP so that uracil cannot be
CC incorporated into DNA. {ECO:0000255|HAMAP-Rule:MF_00635}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00635};
CC -!- PATHWAY: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP
CC route): step 2/2. {ECO:0000255|HAMAP-Rule:MF_00635}.
CC -!- SIMILARITY: Belongs to the dCTP deaminase family. Archaeal dUTPase
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00635}.
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DR EMBL; CP000300; ABE51855.1; -; Genomic_DNA.
DR RefSeq; WP_011499008.1; NC_007955.1.
DR AlphaFoldDB; Q12XH1; -.
DR SMR; Q12XH1; -.
DR STRING; 259564.Mbur_0910; -.
DR EnsemblBacteria; ABE51855; ABE51855; Mbur_0910.
DR GeneID; 3998654; -.
DR KEGG; mbu:Mbur_0910; -.
DR HOGENOM; CLU_103451_2_0_2; -.
DR OMA; WDAGYEG; -.
DR OrthoDB; 94658at2157; -.
DR UniPathway; UPA00610; UER00666.
DR Proteomes; UP000001979; Chromosome.
DR GO; GO:0004170; F:dUTP diphosphatase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006226; P:dUMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd07557; trimeric_dUTPase; 1.
DR Gene3D; 2.70.40.10; -; 1.
DR HAMAP; MF_00635; dUTPase_arch; 1.
DR InterPro; IPR029054; dUTPase-like.
DR InterPro; IPR036157; dUTPase-like_sf.
DR InterPro; IPR023537; dUTPase_archaeal.
DR InterPro; IPR033704; dUTPase_trimeric.
DR Pfam; PF00692; dUTPase; 1.
DR SUPFAM; SSF51283; SSF51283; 1.
PE 3: Inferred from homology;
KW Hydrolase; Nucleotide metabolism; Reference proteome.
FT CHAIN 1..170
FT /note="Probable deoxyuridine 5'-triphosphate
FT nucleotidohydrolase"
FT /id="PRO_1000061431"
SQ SEQUENCE 170 AA; 19122 MW; 77861D19B36ECE59 CRC64;
MTLLSQNELR ELVLANPPLV ENMIDMDTQL QPNGVEMTLK EIRTIKSPGA VDFDNSGRRL
SEGDTIEFNE DGWIHLDPGV YKVLLNEIVN IPKDLAAIAK PRSTLIRCGA TLETAVWDAG
YSGRSECMIV VHNKDGFDLK KDARIMQLLF YHLHTEVEEG YSGSYQNENI
