ID   DUT_MUHVS               Reviewed;         382 AA.
AC   Q69151;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   02-JUN-2021, entry version 68.
DE   RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase {ECO:0000255|HAMAP-Rule:MF_04031};
DE            Short=dUTPase {ECO:0000255|HAMAP-Rule:MF_04031};
DE            EC=3.6.1.23 {ECO:0000255|HAMAP-Rule:MF_04031};
DE   AltName: Full=dUTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_04031};
GN   Name=DUT {ECO:0000255|HAMAP-Rule:MF_04031}; OrderedLocusNames=UL72;
OS   Murid herpesvirus 1 (strain Smith) (MuHV-1) (Mouse cytomegalovirus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Muromegalovirus.
OX   NCBI_TaxID=10367;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7483291; DOI=10.1007/bf01724298;
RA   Messerle M., Rapp M., Lucin P., Koszinowski U.H.;
RT   "Characterization of a conserved gene block in the murine cytomegalovirus
RT   genome.";
RL   Virus Genes 10:73-80(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8971012; DOI=10.1128/jvi.70.12.8833-8849.1996;
RA   Rawlinson W.D., Farrell H.E., Barrell B.G.;
RT   "Analysis of the complete DNA sequence of murine cytomegalovirus.";
RL   J. Virol. 70:8833-8849(1996).
CC   -!- FUNCTION: Involved in nucleotide metabolism: produces dUMP, the
CC       immediate precursor of thymidine nucleotides and decreases the
CC       intracellular concentration of dUTP to avoid uracil incorporation into
CC       viral DNA. {ECO:0000255|HAMAP-Rule:MF_04031}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_04031};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_04031};
CC   -!- PATHWAY: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP
CC       route): step 2/2.
CC   -!- SIMILARITY: Belongs to the dUTPase family. {ECO:0000255|HAMAP-
CC       Rule:MF_04031}.
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DR   EMBL; L07319; AAA96663.1; -; Genomic_DNA.
DR   EMBL; U68299; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; Q69151; -.
DR   UniPathway; UPA00610; UER00666.
DR   Proteomes; UP000008774; Genome.
DR   GO; GO:0004170; F:dUTP diphosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006226; P:dUMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0046080; P:dUTP metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.70.40.10; -; 2.
DR   HAMAP; MF_04031; HSV_DUT; 1.
DR   InterPro; IPR029054; dUTPase-like.
DR   InterPro; IPR036157; dUTPase-like_sf.
DR   InterPro; IPR034745; HSV_DUT.
DR   Pfam; PF00692; dUTPase; 1.
DR   SUPFAM; SSF51283; SSF51283; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nucleotide metabolism;
KW   Reference proteome.
FT   CHAIN           1..382
FT                   /note="Deoxyuridine 5'-triphosphate nucleotidohydrolase"
FT                   /id="PRO_0000182966"
FT   REGION          1..60
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          97..118
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        45..60
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   382 AA;  42885 MW;  0280F70C9B46048D CRC64;
     MIKPNIPAPV RPNLPLSSPD SVLPQAMKDP FNIFDDDEPS EDTSSSEQSS RPPPREMMIS
     SSCALREQTN SSFLNRFFPE DDESSENGAA LAEINQLLRD GPEAPPREPT PKEARRPEKM
     QKIFFQTYGN DFKVEAHGDR LLAQNQRVVR LFERGRILGL GVKMNIPMGY CAVTNCFHPP
     GCVCVMDMAG YGPSDIRAQI VNISSGPLEL PPNMLQIHIH MLPMLLPEPW QTINLMAPHQ
     GDTYFDLRLR RPLSLPPRAS KNLKFEAGHL CEEDARRCLV IPCRHLATKR VLLDPTVWRP
     NSLAVLRVLN ASDEHVDLEA GMAMAKIIFT TPGITPFKPS LTSVMMSFDV PRSDLKLVKG
     GRRDYYRAEE RMRSGRDSSN EG