ID   DUT_ORFN2               Reviewed;         159 AA.
AC   P14597;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase;
DE            Short=dUTPase;
DE            EC=3.6.1.23;
DE   AltName: Full=dUTP pyrophosphatase;
GN   Name=DUT;
OS   Orf virus (strain NZ2) (OV NZ-2).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Parapoxvirus.
OX   NCBI_TaxID=10259;
OH   NCBI_TaxID=9925; Capra hircus (Goat).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9940; Ovis aries (Sheep).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2678731; DOI=10.1016/0042-6822(89)90212-2;
RA   Mercer A.A., Fraser K.M., Stockwell P.A., Robinson A.J.;
RT   "A homologue of retroviral pseudoproteases in the parapoxvirus, orf
RT   virus.";
RL   Virology 172:665-668(1989).
CC   -!- FUNCTION: This enzyme is involved in nucleotide metabolism: it produces
CC       dUMP, the immediate precursor of thymidine nucleotides and it decreases
CC       the intracellular concentration of dUTP so that uracil cannot be
CC       incorporated into DNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the dUTPase family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be a protease-like protein
CC       (pseudoprotease). {ECO:0000305}.
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DR   EMBL; M30023; AAA46786.1; -; Genomic_DNA.
DR   SMR; P14597; -.
DR   GO; GO:0004170; F:dUTP diphosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0006226; P:dUMP biosynthetic process; IEA:InterPro.
DR   GO; GO:0046081; P:dUTP catabolic process; IEA:InterPro.
DR   CDD; cd07557; trimeric_dUTPase; 1.
DR   Gene3D; 2.70.40.10; -; 1.
DR   InterPro; IPR008181; dUTPase.
DR   InterPro; IPR029054; dUTPase-like.
DR   InterPro; IPR036157; dUTPase-like_sf.
DR   InterPro; IPR033704; dUTPase_trimeric.
DR   PANTHER; PTHR11241; PTHR11241; 1.
DR   Pfam; PF00692; dUTPase; 1.
DR   SUPFAM; SSF51283; SSF51283; 1.
DR   TIGRFAMs; TIGR00576; dut; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nucleotide metabolism.
FT   CHAIN           1..159
FT                   /note="Deoxyuridine 5'-triphosphate nucleotidohydrolase"
FT                   /id="PRO_0000182948"
SQ   SEQUENCE   159 AA;  16893 MW;  08CD852D47AE17AD CRC64;
     MEFCHTETLQ VVRLSQNATI PARGSPGAAG LDLCSAYDCV IPSHCSRVVF TDLLIKPPSG
     CYGRIAPRSG LAVKHFIDVG AGVIDEDYRG NVGVVLFNFG NSDFEVKKGD RIAQLICERI
     SCPAVQEVNC LDNTDRGDSG FGSTGSGACG GRDTAWYIS