ADHN_RHOER
ID ADHN_RHOER Reviewed; 22 AA.
AC P81747;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 40.
DE RecName: Full=NDMA-dependent alcohol dehydrogenase {ECO:0000303|PubMed:10784035};
DE Short=NDMA-ADH {ECO:0000303|PubMed:10784035};
DE EC=1.1.99.36 {ECO:0000269|PubMed:10784035};
DE AltName: Full=Nicotinoprotein (NADH-containing) alcohol dehydrogenase {ECO:0000303|PubMed:10784035};
DE Flags: Fragment;
OS Rhodococcus erythropolis (Arthrobacter picolinophilus).
OC Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus;
OC Rhodococcus erythropolis group.
OX NCBI_TaxID=1833;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR,
RP BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, AND SUBUNIT.
RC STRAIN=DSM 1069;
RX PubMed=10784035; DOI=10.1099/00221287-146-4-775;
RA Schenkels P., Duine J.A.;
RT "Nicotinoprotein (NADH-containing) alcohol dehydrogenase from Rhodococcus
RT erythropolis DSM 1069: an efficient catalyst for coenzyme-independent
RT oxidation of a broad spectrum of alcohols and the interconversion of
RT alcohols and aldehydes.";
RL Microbiology 146:775-785(2000).
CC -!- FUNCTION: This is a novel enzyme, catalytically different from common
CC alcohol dehydrogenases. It is effective in oxidizing ethanol, other
CC primary alcohols and benzylalcohol only in the presence of p-nitroso-
CC N,N-dimethylaniline (NDMA) as an electron acceptor. NADH acts as a
CC cofactor here instead of as a coenzyme. {ECO:0000269|PubMed:10784035}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + N,N-dimethyl-4-nitrosoaniline = 4-
CC (hydroxylamino)-N,N-dimethylaniline + an aldehyde;
CC Xref=Rhea:RHEA:48076, ChEBI:CHEBI:15734, ChEBI:CHEBI:17478,
CC ChEBI:CHEBI:59990, ChEBI:CHEBI:59991;
CC Evidence={ECO:0000269|PubMed:10784035};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=A + ethanol = acetaldehyde + AH2; Xref=Rhea:RHEA:33567,
CC ChEBI:CHEBI:13193, ChEBI:CHEBI:15343, ChEBI:CHEBI:16236,
CC ChEBI:CHEBI:17499; EC=1.1.99.36;
CC Evidence={ECO:0000269|PubMed:10784035};
CC -!- COFACTOR:
CC Name=NADH; Xref=ChEBI:CHEBI:57945;
CC Evidence={ECO:0000269|PubMed:10784035};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.1 mM for ethanol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.0037 mM for propan-1-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.01 mM for butan-1-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.0011 mM for heptan-1-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.059 mM for octan-1-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.028 mM for 2-propen-1-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.0072 mM for isobutanol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.0028 mM for 3-methylbutan-1-ol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.0018 mM for benzyl alcohol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.0047 mM for 4-hydroxybenzyl alcohol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.39 mM for vanillyl alcohol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.16 mM for veratryl alcohol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.007 mM for 2-phenylethanol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.056 mM for 2-phenylpropan-1-ol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.014 mM for 3-phenylpropan-1-ol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.0019 mM for 3-(4-hydroxyphenyl)propan-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.0036 mM for cinnamyl alcohol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.0055 mM for coniferyl alcohol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.2 mM for 2-phenylbutan-1-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.0059 mM for 4-(4-methoxyphenyl)butan-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC KM=6 mM for propan-2-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=4.1 mM for butan-2-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=1.3 mM for pentan-2-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.83 mM for hexan-2-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.07 mM for heptan-2-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.12 mM for octan-2-ol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=9.1 mM for 3-methylheptan-4-ol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.28 mM for 4-methylheptan-3-ol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=2.8 mM for 1-phenylethanol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.55 mM for 1-phenylpropan-1-ol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=1.6 mM for 1-phenylpropan-2-ol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.55 mM for 1-phenylbutan-2-ol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.19 mM for cyclohexanol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=35 mM for propane-1,2-diol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=4.1 mM for propane-1,3-diol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=8.3 mM for 3-butene-1,2-diol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=0.2 mM for pentane-1,2-diol (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=3.1 mM for 1-phenylethane-1,2-diol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC KM=0.93 mM for formaldehyde (when NDMA is the electron acceptor)
CC {ECO:0000269|PubMed:10784035};
CC KM=4.95 mM for acetaldehyde (when veratryl alcohol is the electron
CC donor) {ECO:0000269|PubMed:10784035};
CC KM=0.083 mM for benzaldehyde (when veratryl alcohol is the electron
CC donor) {ECO:0000269|PubMed:10784035};
CC KM=0.088 mM for 2-phenylpropionaldehyde (when veratryl alcohol is the
CC electron donor) {ECO:0000269|PubMed:10784035};
CC KM=0.14 mM for 3-phenylpropionaldehyde (when veratryl alcohol is the
CC electron donor) {ECO:0000269|PubMed:10784035};
CC KM=0.12 mM for 3-phenylbutyraldehyde (when veratryl alcohol is the
CC electron donor) {ECO:0000269|PubMed:10784035};
CC KM=242 mM for glyoxal (when veratryl alcohol is the electron donor)
CC {ECO:0000269|PubMed:10784035};
CC KM=3.2 mM for methylglyoxal (when veratryl alcohol is the electron
CC donor) {ECO:0000269|PubMed:10784035};
CC KM=0.21 mM for phenylglyoxal (when veratryl alcohol is the electron
CC donor) {ECO:0000269|PubMed:10784035};
CC KM=38 mM for hydroxyacetone (when veratryl alcohol is the electron
CC donor) {ECO:0000269|PubMed:10784035};
CC KM=1.6 mM for cyclohexanone (when veratryl alcohol is the electron
CC donor) {ECO:0000269|PubMed:10784035};
CC KM=0.0041 mM for NDMA (when veratryl alcohol is the electron donor)
CC {ECO:0000269|PubMed:10784035};
CC Vmax=2.0 umol/min/mg enzyme toward ethanol (when NDMA is the electron
CC acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.5 umol/min/mg enzyme toward propan-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=5.8 umol/min/mg enzyme toward butan-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.0 umol/min/mg enzyme toward pentan-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.2 umol/min/mg enzyme toward hexan-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.7 umol/min/mg enzyme toward heptan-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=3.5 umol/min/mg enzyme toward octan-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=3.0 umol/min/mg enzyme toward 2-propen-1-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=3.3 umol/min/mg enzyme toward isobutanol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=3.1 umol/min/mg enzyme toward 3-methylbutan-1-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.9 umol/min/mg enzyme toward citronellol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=4.9 umol/min/mg enzyme toward benzyl alcohol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.79 umol/min/mg enzyme toward 4-hydroxybenzyl alcohol (when
CC NDMA is the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.0 umol/min/mg enzyme toward vanillyl alcohol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.8 umol/min/mg enzyme toward veratryl alcohol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.6 umol/min/mg enzyme toward 2-phenylethanol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.8 umol/min/mg enzyme toward 2-phenylpropan-1-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.4 umol/min/mg enzyme toward 3-phenylpropan-1-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.7 umol/min/mg enzyme toward 3-(4-hydroxyphenyl)propan-1-ol
CC (when NDMA is the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=3.4 umol/min/mg enzyme toward cinnamyl alcohol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.92 umol/min/mg enzyme toward coniferyl alcohol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.3 umol/min/mg enzyme toward 2-phenylbutan-1-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=4.0 umol/min/mg enzyme toward 4-(4-methoxyphenyl)butan-1-ol
CC (when NDMA is the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.16 umol/min/mg enzyme toward propan-2-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.81 umol/min/mg enzyme toward butan-2-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.0 umol/min/mg enzyme toward pentan-2-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.6 umol/min/mg enzyme toward hexan-2-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.1 umol/min/mg enzyme toward heptan-2-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.75 umol/min/mg enzyme toward octan-2-ol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=6.6 umol/min/mg enzyme toward 3-methylheptan-4-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.58 umol/min/mg enzyme toward 4-methylheptan-3-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.2 umol/min/mg enzyme toward 1-phenylethanol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.84 umol/min/mg enzyme toward 1-phenylpropan-1-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.9 umol/min/mg enzyme toward 1-phenylpropan-2-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.5 umol/min/mg enzyme toward 1-phenylbutan-2-ol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.9 umol/min/mg enzyme toward cyclohexanol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.3 umol/min/mg enzyme toward propane-1,2-diol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=3.0 umol/min/mg enzyme toward propane-1,3-diol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.6 umol/min/mg enzyme toward 3-butene-1,2-diol (when NDMA is
CC the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.0 umol/min/mg enzyme toward pentane-1,2-diol (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.4 umol/min/mg enzyme toward 1-phenylethane-1,2-diol (when NDMA
CC is the electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=3.4 umol/min/mg enzyme toward formaldehyde (when NDMA is the
CC electron acceptor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.1 umol/min/mg enzyme toward acetaldehyde (when veratryl
CC alcohol is the electron donor) {ECO:0000269|PubMed:10784035};
CC Vmax=3.7 umol/min/mg enzyme toward benzaldehyde (when veratryl
CC alcohol is the electron donor) {ECO:0000269|PubMed:10784035};
CC Vmax=2.38 umol/min/mg enzyme toward 2-phenylpropionaldehyde (when
CC veratryl alcohol is the electron donor)
CC {ECO:0000269|PubMed:10784035};
CC Vmax=8.32 umol/min/mg enzyme toward 3-phenylpropionaldehyde (when
CC veratryl alcohol is the electron donor)
CC {ECO:0000269|PubMed:10784035};
CC Vmax=8.52 umol/min/mg enzyme toward 3-phenylbutyraldehyde (when
CC veratryl alcohol is the electron donor)
CC {ECO:0000269|PubMed:10784035};
CC Vmax=1.0 umol/min/mg enzyme toward glyoxal (when veratryl alcohol is
CC the electron donor) {ECO:0000269|PubMed:10784035};
CC Vmax=4.5 umol/min/mg enzyme toward methylglyoxal (when veratryl
CC alcohol is the electron donor) {ECO:0000269|PubMed:10784035};
CC Vmax=5.1 umol/min/mg enzyme toward phenylglyoxal (when veratryl
CC alcohol is the electron donor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.15 umol/min/mg enzyme toward hydroxyacetone (when veratryl
CC alcohol is the electron donor) {ECO:0000269|PubMed:10784035};
CC Vmax=0.097 umol/min/mg enzyme toward cyclohexanone (when veratryl
CC alcohol is the electron donor) {ECO:0000269|PubMed:10784035};
CC Vmax=1.7 umol/min/mg enzyme toward NDMA (when veratryl alcohol is the
CC electron donor) {ECO:0000269|PubMed:10784035};
CC Note=The lower limit of the determination of KM with NDMA as electron
CC acceptor was 0.001 mM. KM was even lower for hexan-1-ol, pentan-1-ol,
CC and citronellol.;
CC pH dependence:
CC Optimum pH is 7.0. {ECO:0000269|PubMed:10784035};
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:10784035}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC family. {ECO:0000305}.
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DR AlphaFoldDB; P81747; -.
DR SABIO-RK; P81747; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase.
FT CHAIN 1..>22
FT /note="NDMA-dependent alcohol dehydrogenase"
FT /id="PRO_0000064454"
FT NON_TER 22
SQ SEQUENCE 22 AA; 2574 MW; 6B33E6753F537A17 CRC64;
MKTKAAVLFE THKPFEIVEL EL
