ADHP_ECOLI
ID ADHP_ECOLI Reviewed; 336 AA.
AC P39451; P76126; P78157; P78268;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Alcohol dehydrogenase, propanol-preferring;
DE EC=1.1.1.1;
GN Name=adhP; Synonyms=yddN; OrderedLocusNames=b1478, JW1474;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PROTEIN SEQUENCE OF 1-35, AND CHARACTERIZATION.
RA Harayama S.;
RL Submitted (OCT-1994) to UniProtKB.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 87-118.
RX PubMed=2158980; DOI=10.1128/jb.172.5.2774-2778.1990;
RA Kubo K.M., Craig N.L.;
RT "Bacterial transposon Tn7 utilizes two different classes of target sites.";
RL J. Bacteriol. 172:2774-2778(1990).
CC -!- FUNCTION: Preferred specificity is towards 1-propanol.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC family. {ECO:0000305}.
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DR EMBL; U00096; AAC74551.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15126.1; -; Genomic_DNA.
DR EMBL; M31532; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A64901; A64901.
DR RefSeq; NP_415995.4; NC_000913.3.
DR RefSeq; WP_000642433.1; NZ_SSZK01000038.1.
DR PDB; 4GKV; X-ray; 2.01 A; A/B/C/D=1-336.
DR PDBsum; 4GKV; -.
DR AlphaFoldDB; P39451; -.
DR SMR; P39451; -.
DR BioGRID; 4262899; 24.
DR IntAct; P39451; 5.
DR STRING; 511145.b1478; -.
DR jPOST; P39451; -.
DR PaxDb; P39451; -.
DR PRIDE; P39451; -.
DR EnsemblBacteria; AAC74551; AAC74551; b1478.
DR EnsemblBacteria; BAA15126; BAA15126; BAA15126.
DR GeneID; 946036; -.
DR KEGG; ecj:JW1474; -.
DR KEGG; eco:b1478; -.
DR PATRIC; fig|1411691.4.peg.789; -.
DR EchoBASE; EB2506; -.
DR eggNOG; COG1064; Bacteria.
DR HOGENOM; CLU_026673_20_1_6; -.
DR InParanoid; P39451; -.
DR OMA; GLKMTDT; -.
DR PhylomeDB; P39451; -.
DR BioCyc; EcoCyc:ADHP-MON; -.
DR BioCyc; MetaCyc:ADHP-MON; -.
DR PRO; PR:P39451; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IDA:EcoCyc.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IDA:EcoCyc.
DR GO; GO:0046187; P:acetaldehyde catabolic process; IDA:EcoliWiki.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0045471; P:response to ethanol; IEP:EcoliWiki.
DR InterPro; IPR013149; ADH-like_C.
DR InterPro; IPR013154; ADH_N.
DR InterPro; IPR002328; ADH_Zn_CS.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020843; PKS_ER.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF00107; ADH_zinc_N; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SUPFAM; SSF50129; SSF50129; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00059; ADH_ZINC; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Metal-binding; NAD;
KW Oxidoreductase; Reference proteome; Zinc.
FT CHAIN 1..336
FT /note="Alcohol dehydrogenase, propanol-preferring"
FT /id="PRO_0000160740"
FT BINDING 37
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 58
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 89
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 92
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 95
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 103
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 145
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT CONFLICT 34
FT /note="C -> P (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 90
FT /note="G -> R (in Ref. 5; M31532)"
FT /evidence="ECO:0000305"
FT CONFLICT 104
FT /note="R -> P (in Ref. 5; M31532)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="N -> K (in Ref. 5; M31532)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="S -> G (in Ref. 5; M31532)"
FT /evidence="ECO:0000305"
FT CONFLICT 117..118
FT /note="MA -> RV (in Ref. 5; M31532)"
FT /evidence="ECO:0000305"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 12..16
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 26..35
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 38..45
FT /evidence="ECO:0007829|PDB:4GKV"
FT TURN 46..48
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 58..67
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 79..87
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 90..92
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 95..97
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 100..102
FT /evidence="ECO:0007829|PDB:4GKV"
FT TURN 109..111
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 116..124
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 125..127
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 137..143
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 146..156
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 164..168
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 172..183
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 188..194
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 196..204
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 208..212
FT /evidence="ECO:0007829|PDB:4GKV"
FT TURN 213..215
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 218..226
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 227..234
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 239..248
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 249..257
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 262..268
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 269..274
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 278..281
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 287..298
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 306..309
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 311..313
FT /evidence="ECO:0007829|PDB:4GKV"
FT HELIX 314..322
FT /evidence="ECO:0007829|PDB:4GKV"
FT STRAND 327..333
FT /evidence="ECO:0007829|PDB:4GKV"
SQ SEQUENCE 336 AA; 35380 MW; 8A9080F273866947 CRC64;
MKAAVVTKDH HVDVTYKTLR SLKHGEALLK MECCGVCHTD LHVKNGDFGD KTGVILGHEG
IGVVAEVGPG VTSLKPGDRA SVAWFYEGCG HCEYCNSGNE TLCRSVKNAG YSVDGGMAEE
CIVVADYAVK VPDGLDSAAA SSITCAGVTT YKAVKLSKIR PGQWIAIYGL GGLGNLALQY
AKNVFNAKVI AIDVNDEQLK LATEMGADLA INSHTEDAAK IVQEKTGGAH AAVVTAVAKA
AFNSAVDAVR AGGRVVAVGL PPESMSLDIP RLVLDGIEVV GSLVGTRQDL TEAFQFAAEG
KVVPKVALRP LADINTIFTE MEEGKIRGRM VIDFRH
