DWRF8_MAIZE
ID DWRF8_MAIZE Reviewed; 630 AA.
AC Q9ST48; Q93V57; Q93VT0; Q93VT1; Q93VT2; Q93WG1; Q93WI7; Q945I5; Q945I6;
AC Q945I7; Q945I8; Q945I9; Q945J0; Q945J1; Q945J2; Q945J3; Q945J4; Q945J5;
AC Q945J6;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=DELLA protein DWARF8;
DE Short=Protein dwarf-8;
GN Name=D8;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS D8-D1 55-ASP--LYS-59 DELINS
RP GLY AND D8-2023 87-LEU--ASP-98 DEL.
RX PubMed=10421366; DOI=10.1038/22307;
RA Peng J., Richards D.E., Hartley N.M., Murphy G.P., Devos K.M.,
RA Flintham J.E., Beales J., Fish L.J., Worland A.J., Pelica F., Sudhakar D.,
RA Christou P., Snape J.W., Gale M.D., Harberd N.P.;
RT "'Green revolution' genes encode mutant gibberellin response modulators.";
RL Nature 400:256-261(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-581, AND VARIANTS.
RC STRAIN=cv. 38-11, cv. A272, cv. A619, cv. A632, cv. B103, cv. B104,
RC cv. B37, cv. B68, cv. B73, cv. C103, cv. CM105, cv. CM174, cv. CML258,
RC cv. CML281, cv. CML5, cv. CMV3, cv. EP1, cv. F2, cv. F2834T, cv. F7,
RC cv. GT112, cv. H99, cv. I137TN, cv. I29, cv. KUI21, cv. KUI3, cv. KUI43,
RC cv. M162W, cv. M37W, cv. Missouri 17, cv. Missouri 24W, cv. MS153,
RC cv. N192, cv. ND246, cv. North Carolina 250, cv. North Carolina 260,
RC cv. North Carolina 304, cv. North Carolina 320, cv. North Carolina 348,
RC cv. North Carolina 350, cv. North Carolina 352, cv. North Carolina 354,
RC cv. Ohio 43, cv. Ohio 7B, cv. P39, cv. PA91, cv. Q6199, cv. SA24,
RC cv. SC213, cv. SG18, cv. Tennessee 232, cv. Texas 601, cv. Tzi10,
RC cv. Tzi18, cv. U267Y, cv. WF9, cv. Wisconsin 117HT, cv. Wisconsin 182B, and
RC cv. Wisconsin 64A;
RX PubMed=11562485; DOI=10.1073/pnas.201394398;
RA Remington D.L., Thornsberry J.M., Matsuoka Y., Wilson L.M., Whitt S.R.,
RA Doebley J., Kresovich S., Goodman M.M., Buckler E.S. IV;
RT "Structure of linkage disequilibrium and phenotypic associations in the
RT maize genome.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11479-11484(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 169-434.
RX PubMed=11470895; DOI=10.1073/pnas.151244298;
RA Tenaillon M.I., Sawkins M.C., Long A.D., Gaut R.L., Doebley J.F.,
RA Gaut B.S.;
RT "Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea
RT mays ssp. mays L.).";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9161-9166(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 477-630.
RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K.,
RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.;
RT "Maize full-length cDNA project.";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP POLYMORPHISM.
RX PubMed=11431702; DOI=10.1038/90135;
RA Thornsberry J.M., Goodman M.M., Doebley J., Kresovich S., Nielsen D.,
RA Buckler E.S. IV;
RT "Dwarf8 polymorphisms associate with variation in flowering time.";
RL Nat. Genet. 28:286-289(2001).
RN [6]
RP POLYMORPHISM.
RX PubMed=15933874; DOI=10.1007/s00122-005-1996-6;
RA Andersen J.R., Schrag T., Melchinger A.E., Zein I., Luebberstedt T.;
RT "Validation of Dwarf8 polymorphisms associated with flowering time in elite
RT European inbred lines of maize (Zea mays L.).";
RL Theor. Appl. Genet. 111:206-217(2005).
CC -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC of the gibberellin (GA) signaling pathway. Probably acts by
CC participating in large multiprotein complexes that repress
CC transcription of GA-inducible genes. Upon GA application, it is
CC degraded by the proteasome, allowing the GA signaling pathway.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC {ECO:0000305}.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC its subsequent degradation (Probable). {ECO:0000305}.
CC -!- POLYMORPHISM: Variations between cultivars affect flowering time and
CC plant height.
CC -!- BIOTECHNOLOGY: This gene, also known as the 'green revolution gene' has
CC been introduced by conventional breeding procedures into several
CC cultivars. It produces shorter plants, increase grain yield at the
CC expense of straw biomass, and are more resistant to damage by wind and
CC rain.
CC -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=All things dwarfed and
CC beautiful - Issue 70 of May 2006;
CC URL="https://web.expasy.org/spotlight/back_issues/070";
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DR EMBL; AJ242530; CAB51557.1; -; Genomic_DNA.
DR EMBL; AF413112; AAL10302.1; -; Genomic_DNA.
DR EMBL; AF413113; AAL10303.1; -; Genomic_DNA.
DR EMBL; AF413114; AAL10304.1; -; Genomic_DNA.
DR EMBL; AF413115; AAL10305.1; -; Genomic_DNA.
DR EMBL; AF413116; AAL10306.1; -; Genomic_DNA.
DR EMBL; AF413117; AAL10307.1; -; Genomic_DNA.
DR EMBL; AF413118; AAL10308.1; -; Genomic_DNA.
DR EMBL; AF413119; AAL10309.1; -; Genomic_DNA.
DR EMBL; AF413120; AAL10310.1; -; Genomic_DNA.
DR EMBL; AF413121; AAL10311.1; -; Genomic_DNA.
DR EMBL; AF413122; AAL10312.1; -; Genomic_DNA.
DR EMBL; AF413123; AAL10313.1; -; Genomic_DNA.
DR EMBL; AF413124; AAL10314.1; -; Genomic_DNA.
DR EMBL; AF413125; AAL10315.1; -; Genomic_DNA.
DR EMBL; AF413126; AAL10316.1; -; Genomic_DNA.
DR EMBL; AF413127; AAL10317.1; -; Genomic_DNA.
DR EMBL; AF413128; AAL10318.1; -; Genomic_DNA.
DR EMBL; AF413129; AAL10319.1; -; Genomic_DNA.
DR EMBL; AF413130; AAL10320.1; -; Genomic_DNA.
DR EMBL; AF413131; AAL10321.1; -; Genomic_DNA.
DR EMBL; AF413132; AAL10322.1; -; Genomic_DNA.
DR EMBL; AF413133; AAL10323.1; -; Genomic_DNA.
DR EMBL; AF413134; AAL10324.1; -; Genomic_DNA.
DR EMBL; AF413135; AAL10325.1; -; Genomic_DNA.
DR EMBL; AF413136; AAL10326.1; -; Genomic_DNA.
DR EMBL; AF413137; AAL10327.1; -; Genomic_DNA.
DR EMBL; AF413138; AAL10328.1; -; Genomic_DNA.
DR EMBL; AF413139; AAL10329.1; -; Genomic_DNA.
DR EMBL; AF413140; AAL10330.1; -; Genomic_DNA.
DR EMBL; AF413141; AAL10331.1; -; Genomic_DNA.
DR EMBL; AF413142; AAL10332.1; -; Genomic_DNA.
DR EMBL; AF413143; AAL10333.1; -; Genomic_DNA.
DR EMBL; AF413144; AAL10334.1; -; Genomic_DNA.
DR EMBL; AF413145; AAL10335.1; -; Genomic_DNA.
DR EMBL; AF413146; AAL10336.1; -; Genomic_DNA.
DR EMBL; AF413147; AAL10337.1; -; Genomic_DNA.
DR EMBL; AF413148; AAL10338.1; -; Genomic_DNA.
DR EMBL; AF413149; AAL10339.1; -; Genomic_DNA.
DR EMBL; AF413150; AAL10340.1; -; Genomic_DNA.
DR EMBL; AF413151; AAL10341.1; -; Genomic_DNA.
DR EMBL; AF413152; AAL10342.1; -; Genomic_DNA.
DR EMBL; AF413153; AAL10343.1; -; Genomic_DNA.
DR EMBL; AF413154; AAL10344.1; -; Genomic_DNA.
DR EMBL; AF413155; AAL10345.1; -; Genomic_DNA.
DR EMBL; AF413156; AAL10346.1; -; Genomic_DNA.
DR EMBL; AF413157; AAL10347.1; -; Genomic_DNA.
DR EMBL; AF413158; AAL10348.1; -; Genomic_DNA.
DR EMBL; AF413159; AAL10349.1; -; Genomic_DNA.
DR EMBL; AF413160; AAL10350.1; -; Genomic_DNA.
DR EMBL; AF413161; AAL10351.1; -; Genomic_DNA.
DR EMBL; AF413162; AAL10352.1; -; Genomic_DNA.
DR EMBL; AF413163; AAL10353.1; -; Genomic_DNA.
DR EMBL; AF413164; AAL10354.1; -; Genomic_DNA.
DR EMBL; AF413165; AAL10355.1; -; Genomic_DNA.
DR EMBL; AF413166; AAL10356.1; -; Genomic_DNA.
DR EMBL; AF413167; AAL10357.1; -; Genomic_DNA.
DR EMBL; AF413168; AAL10358.1; -; Genomic_DNA.
DR EMBL; AF413169; AAL10359.1; -; Genomic_DNA.
DR EMBL; AF413170; AAL10360.1; -; Genomic_DNA.
DR EMBL; AF413171; AAL10361.1; -; Genomic_DNA.
DR EMBL; AF413172; AAL10362.1; -; Genomic_DNA.
DR EMBL; AF413173; AAL10363.1; -; Genomic_DNA.
DR EMBL; AF413174; AAL10364.1; -; Genomic_DNA.
DR EMBL; AF413175; AAL10365.1; -; Genomic_DNA.
DR EMBL; AF413176; AAL10366.1; -; Genomic_DNA.
DR EMBL; AF413177; AAL10367.1; -; Genomic_DNA.
DR EMBL; AF413178; AAL10368.1; -; Genomic_DNA.
DR EMBL; AF413179; AAL10369.1; -; Genomic_DNA.
DR EMBL; AF413180; AAL10370.1; -; Genomic_DNA.
DR EMBL; AF413181; AAL10371.1; -; Genomic_DNA.
DR EMBL; AF413182; AAL10372.1; -; Genomic_DNA.
DR EMBL; AF413183; AAL10373.1; -; Genomic_DNA.
DR EMBL; AF413184; AAL10374.1; -; Genomic_DNA.
DR EMBL; AF413185; AAL10375.1; -; Genomic_DNA.
DR EMBL; AF413186; AAL10376.1; -; Genomic_DNA.
DR EMBL; AF413187; AAL10377.1; -; Genomic_DNA.
DR EMBL; AF413188; AAL10378.1; -; Genomic_DNA.
DR EMBL; AF413189; AAL10379.1; -; Genomic_DNA.
DR EMBL; AF413190; AAL10380.1; -; Genomic_DNA.
DR EMBL; AF413191; AAL10381.1; -; Genomic_DNA.
DR EMBL; AF413192; AAL10382.1; -; Genomic_DNA.
DR EMBL; AF413193; AAL10383.1; -; Genomic_DNA.
DR EMBL; AF413194; AAL10384.1; -; Genomic_DNA.
DR EMBL; AF413195; AAL10385.1; -; Genomic_DNA.
DR EMBL; AF413196; AAL10386.1; -; Genomic_DNA.
DR EMBL; AF413197; AAL10387.1; -; Genomic_DNA.
DR EMBL; AF413198; AAL10388.1; -; Genomic_DNA.
DR EMBL; AF413199; AAL10389.1; -; Genomic_DNA.
DR EMBL; AF413200; AAL10390.1; -; Genomic_DNA.
DR EMBL; AF413201; AAL10391.1; -; Genomic_DNA.
DR EMBL; AF413202; AAL10392.1; -; Genomic_DNA.
DR EMBL; AF413203; AAL10393.1; -; Genomic_DNA.
DR EMBL; AF377621; AAK59901.1; -; Genomic_DNA.
DR EMBL; AF377622; AAK59902.1; -; Genomic_DNA.
DR EMBL; AF377623; AAK59903.1; -; Genomic_DNA.
DR EMBL; AF377624; AAK59904.1; -; Genomic_DNA.
DR EMBL; AF377625; AAK59905.1; -; Genomic_DNA.
DR EMBL; AF377626; AAK59906.1; -; Genomic_DNA.
DR EMBL; AF377627; AAK59907.1; -; Genomic_DNA.
DR EMBL; AF377628; AAK59908.1; -; Genomic_DNA.
DR EMBL; AF377629; AAK59909.1; -; Genomic_DNA.
DR EMBL; AF377630; AAK59910.1; -; Genomic_DNA.
DR EMBL; AF377631; AAK59911.1; -; Genomic_DNA.
DR EMBL; AF377632; AAK59912.1; -; Genomic_DNA.
DR EMBL; AF377633; AAK59913.1; -; Genomic_DNA.
DR EMBL; AF377634; AAK59914.1; -; Genomic_DNA.
DR EMBL; AF377635; AAK59915.1; -; Genomic_DNA.
DR EMBL; AF377636; AAK59916.1; -; Genomic_DNA.
DR EMBL; AF377637; AAK59917.1; -; Genomic_DNA.
DR EMBL; AF377638; AAK59918.1; -; Genomic_DNA.
DR EMBL; AF377639; AAK59919.1; -; Genomic_DNA.
DR EMBL; AF377640; AAK59920.1; -; Genomic_DNA.
DR EMBL; AF377641; AAK59921.1; -; Genomic_DNA.
DR EMBL; AF377642; AAK59922.1; -; Genomic_DNA.
DR EMBL; AF377643; AAK59923.1; -; Genomic_DNA.
DR EMBL; AF377644; AAK59924.1; -; Genomic_DNA.
DR EMBL; AF377645; AAK59925.1; -; Genomic_DNA.
DR EMBL; DR799058; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; Q9ST48; -.
DR SMR; Q9ST48; -.
DR STRING; 4577.GRMZM2G144744_P01; -.
DR PaxDb; Q9ST48; -.
DR EnsemblPlants; Zm00001eb054480_T001; Zm00001eb054480_P001; Zm00001eb054480.
DR Gramene; Zm00001eb054480_T001; Zm00001eb054480_P001; Zm00001eb054480.
DR MaizeGDB; 12143; -.
DR eggNOG; ENOG502QPMG; Eukaryota.
DR OMA; VQQENFA; -.
DR Proteomes; UP000007305; Chromosome 1.
DR ExpressionAtlas; Q9ST48; baseline and differential.
DR Genevisible; Q9ST48; ZM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.1290; -; 1.
DR InterPro; IPR038088; DELLA_N_sf.
DR InterPro; IPR030006; TF_DELLA.
DR InterPro; IPR021914; TF_DELLA_N.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF47; PTHR31636:SF47; 1.
DR Pfam; PF12041; DELLA; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Gibberellin signaling pathway; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..630
FT /note="DELLA protein DWARF8"
FT /id="PRO_0000132246"
FT DOMAIN 234..623
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 161..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..297
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 316..381
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 395..427
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 443..544
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 547..623
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 38..42
FT /note="DELLA motif"
FT MOTIF 248..252
FT /note="LxCxE motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 347..351
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 451..455
FT /note="LXXLL motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 186..206
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 12
FT /note="G -> D (in strain: cv. CMV3)"
FT VARIANT 13
FT /note="G -> GG (in strain: cv. CML258, cv. CML5, cv. NC354
FT and cv. Q6199)"
FT VARIANT 28
FT /note="G -> V (in strain: cv. H99, cv. NC260, cv. PA91, cv.
FT U267Y, cv. W64A and cv. WF9)"
FT VARIANT 55..59
FT /note="DVAQK -> G (in D8-d1; induces a dwarf phenotype)"
FT /evidence="ECO:0000269|PubMed:10421366"
FT VARIANT 87..98
FT /note="Missing (in D8-2023; induces a dwarf phenotype)"
FT /evidence="ECO:0000269|PubMed:10421366"
FT VARIANT 231
FT /note="Missing (in strain: cv. CM7)"
FT VARIANT 255
FT /note="Q -> H (in strain: cv. MO24W)"
FT VARIANT 304
FT /note="S -> R (in strain: cv. B103)"
FT VARIANT 390
FT /note="T -> I (in strain: cv. EP1)"
FT VARIANT 413
FT /note="Y -> C (in strain: cv. CMV3)"
FT VARIANT 475
FT /note="I -> T (in strain: cv. GT112)"
FT VARIANT 480
FT /note="E -> K (in strain: cv. H99)"
FT VARIANT 484
FT /note="N -> S (in strain: cv. NC260)"
FT VARIANT 486
FT /note="N -> D (in strain: cv. KUI43)"
FT VARIANT 500
FT /note="Y -> H (in strain: cv. NC320)"
FT VARIANT 505
FT /note="F -> S (in strain: cv. NC250)"
FT VARIANT 510..511
FT /note="Missing (in strain: cv. A619, cv. CM105, cv. CM174,
FT cv. CMV3, cv. F7, cv. F2834T, cv. N192, cv. Ohio 43 and cv.
FT P39)"
FT VARIANT 519
FT /note="T -> A (in strain: cv. A619, cv. CM105, cv. CM7, cv.
FT CM174, cv. CMV3, cv. F2834T, cv. F7, cv. N192, cv. Ohio 43
FT and cv. P39)"
FT VARIANT 533
FT /note="M -> K (in strain: cv. F2)"
FT VARIANT 578
FT /note="G -> S (in strain: cv. M37W and cv. Tzi10)"
SQ SEQUENCE 630 AA; 66029 MW; 3D56851726C51042 CRC64;
MKREYQDAGG SGGDMGSSKD KMMAAAAGAG EQEEEDVDEL LAALGYKVRS SDMADVAQKL
EQLEMAMGMG GVGGAGATAD DGFVSHLATD TVHYNPSDLS SWVESMLSEL NAPPAPLPPA
TPAPRLASTS STVTSGAAAG AGYFDLPPAV DSSSSTYALK PIPSPVAAPS ADPSTDSARE
PKRMRTGGGS TSSSSSSSSS MDGGRTRSSV VEAAPPATQA SAAANGPAVP VVVVDTQEAG
IRLVHALLAC AEAVQQENFS AAEALVKQIP MLASSQGGAM RKVAAYFGEA LARRVYRFRP
PPDSSLLDAA FADLLHAHFY ESCPYLKFAH FTANQAILEA FAGCRRVHVV DFGIKQGMQW
PALLQALALR PGGPPSFRLT GVGPPQPDET DALQQVGWKL AQFAHTIRVD FQYRGLVAAT
LADLEPFMLQ PEGDDTDDEP EVIAVNSVFE LHRLLAQPGA LEKVLGTVRA VRPRIVTVVE
QEANHNSGTF LDRFTESLHY YSTMFDSLEG AGAGSGQSTD ASPAAAGGTD QVMSEVYLGR
QICNVVACEG AERTERHETL GQWRSRLGGS GFAPVHLGSN AYKQASTLLA LFAGGDGYRV
EEKDGCLTLG WHTRPLIATS AWRVAAAAAP
