ID   ADH_DROAN               Reviewed;         256 AA.
AC   Q50L96; Q8N0A0;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Alcohol dehydrogenase {ECO:0000250|UniProtKB:P00334};
DE            EC=1.1.1.1;
GN   Name=Adh {ECO:0000312|EMBL:BAD98197.1}; ORFNames=GF14888;
OS   Drosophila ananassae (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7217;
RN   [1] {ECO:0000312|EMBL:BAD98197.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16143626; DOI=10.1534/genetics.105.041699;
RA   Nozawa M., Aotsuka T., Tamura K.;
RT   "A novel chimeric gene, siren, with retroposed promoter sequence in the
RT   Drosophila bipectinata complex.";
RL   Genetics 171:1719-1727(2005).
RN   [2] {ECO:0000312|EMBL:ACF95828.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Jones C.D., Shih H.-J.;
RT   "Patterns of amino acid evolution in Drosophila ananassae chimeric gene,
RT   siren, parallel those of other Adh derived chimeras.";
RL   Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000312|EMBL:EDV30743.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14024-0371.13 {ECO:0000269|PubMed:17994087};
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
RN   [4] {ECO:0000312|EMBL:AAM28691.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-145.
RX   AGRICOLA=IND23292123;
RA   Schawaroch V.A.;
RT   "Phylogeny of a paradigm lineage: the Drosophila melanogaster species group
RT   (Diptera: Drosophilidae).";
RL   Biol. J. Linn. Soc. Lond. 76:21-37(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC         Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10001, ECO:0000305};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC         Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC         ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10001, ECO:0000305};
CC   -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000255}.
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DR   EMBL; AB194426; BAD98197.1; -; Genomic_DNA.
DR   EMBL; EU877943; ACF95828.1; -; Genomic_DNA.
DR   EMBL; CH902620; EDV30743.1; -; Genomic_DNA.
DR   EMBL; AF459754; AAM28691.1; -; Genomic_DNA.
DR   RefSeq; XP_001961522.1; XM_001961486.2.
DR   AlphaFoldDB; Q50L96; -.
DR   SMR; Q50L96; -.
DR   STRING; 7217.FBpp0118080; -.
DR   EnsemblMetazoa; FBtr0119588; FBpp0118080; FBgn0012113.
DR   GeneID; 6492907; -.
DR   KEGG; dan:6492907; -.
DR   eggNOG; KOG4169; Eukaryota.
DR   HOGENOM; CLU_010194_2_16_1; -.
DR   InParanoid; Q50L96; -.
DR   OMA; TTLQCAQ; -.
DR   OrthoDB; 1053465at2759; -.
DR   PhylomeDB; Q50L96; -.
DR   ChiTaRS; Adh; fly.
DR   Proteomes; UP000007801; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblMetazoa.
DR   GO; GO:0032991; C:protein-containing complex; IEA:EnsemblMetazoa.
DR   GO; GO:0008774; F:acetaldehyde dehydrogenase (acetylating) activity; IEA:EnsemblMetazoa.
DR   GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:EnsemblMetazoa.
DR   GO; GO:0006117; P:acetaldehyde metabolic process; IEA:EnsemblMetazoa.
DR   GO; GO:0046164; P:alcohol catabolic process; ISS:UniProtKB.
DR   GO; GO:0048149; P:behavioral response to ethanol; IEA:EnsemblMetazoa.
DR   GO; GO:0006069; P:ethanol oxidation; IEA:EnsemblMetazoa.
DR   GO; GO:0006734; P:NADH metabolic process; IEA:EnsemblMetazoa.
DR   InterPro; IPR002425; ADH_Drosophila-type.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR01168; ALCDHDRGNASE.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P00334"
FT   CHAIN           2..256
FT                   /note="Alcohol dehydrogenase"
FT                   /evidence="ECO:0000250|UniProtKB:P00334"
FT                   /id="PRO_0000352752"
FT   ACT_SITE        153
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P00334,
FT                   ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         12..41
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P00334"
FT   BINDING         140
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P00334"
SQ   SEQUENCE   256 AA;  27854 MW;  D9702C0DA79765E0 CRC64;
     MALSLTNKNV VFVAGLGGIG LDTTKELLKR DLKNLVILDR IDNPVVIAEL KTINPKVTVT
     FIPYDVTVPI TETKKLLKTI FDKLKTVDIL INGAGILDDH QIERTIAVNY TGLVNTTTAI
     LDFWDKRKGG PGGIICNIGS VTGFNAIYQV PVYSGTKAAV VNFTSSLAKL APITGVTAYT
     VNPGITRTTL VHKFNSWLDV EPQVAEKLLA HPTQSSQACG ENFVKAIELN QNGAIWKLDR
     GTLEAIQWSK HWDSGI